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Composition of and variation in high- and low-molecular weight glutenin subunits, and omega gliadins in Ethiopian tetraploid wheat germplasm

Published online by Cambridge University Press:  12 February 2007

Faris Hailu*
Affiliation:
University of Lackomelza, Office of PIC, P.O. Box 33809, Addis Ababa, Ethiopia
Eva Johansson
Affiliation:
Department of Crop Science, Swedish University of Agricultural Sciences, P.O. Box 44, SE-230 53 Alnarp, Sweden
Arnulf Merker
Affiliation:
Department of Crop Science, Swedish University of Agricultural Sciences, P.O. Box 44, SE-230 53 Alnarp, Sweden
Getachew Belay
Affiliation:
Debre Zeit Agricultural Research Centre, P.O. Box 32, Debre Zeit, Ethiopia
Harjit-Singh
Affiliation:
Department of Plant Science, Alemaya University, P.O. Box 219, Alemaya, Ethiopia
Habtamu Zeleke
Affiliation:
Department of Plant Science, Alemaya University, P.O. Box 219, Alemaya, Ethiopia
*
*Corresponding author: E-mail: Markhmets@yahoo.com

Abstract

A collection of 120 Ethiopian tetraploid wheat accessions was analysed for high-molecular weight (HMW) glutenin subunit, low-molecular weight (LMW) glutenin subunit and omega gliadin composition by SDS–PAGE. For the HMW glutenin subunits, a new allelic variant, 2****, was detected which has not been previously described at the Glu-A1 locus. A high proportion of Glu-A1x banding pattern was observed in durum wheat. For the Glu-B1 locus four different banding patterns were detected. Among those HMW glutenin subunits, 7+8 were the most common, while subunits 14+15 and 6+8 were found to be rare. A high degree of variation was evident for the LMW glutenin subunits and D-zone omega gliadins. The association of the composition of the gluten with quality has been discussed. This wide variation can be used in improving the quality of wheat and to widen its genetic base.

Type
Research Article
Copyright
Copyright © NIAB 2006

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