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Characterization of 1Sty13, a novel high-molecular-weight glutenin subunit from Elymus sibiricus L.

Published online by Cambridge University Press:  25 May 2021

Mei Yan
Affiliation:
School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou Province, China Shandong Luyan Agricultural Co., LTC, Jinan, Shandong Province, China
Muzi Li
Affiliation:
School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou Province, China
Zaidong Yang
Affiliation:
Shandong Luyan Agricultural Co., LTC, Jinan, Shandong Province, China
Feng Yu*
Affiliation:
School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou Province, China
Xuye Du*
Affiliation:
School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou Province, China
*
*Corresponding authors. E-mail: yufengcaocao@163.com, duxuye@gznu.edu.cn
*Corresponding authors. E-mail: yufengcaocao@163.com, duxuye@gznu.edu.cn

Abstract

High-molecular-weight glutenin subunit (HMW-GS) is a key factor affecting dough-processing quality. 1Sty13 is a novel HMW-GS found in the tetraploid species, Elymus sibiricus L. 1Sty13 has faster electrophoretic mobility than the 1Dy12 subunit on sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis. The gene encoding the 1Sty13 subunit was composed of 1803 nucleotide base pairs with an open reading frame that was 599 amino acids in length. Analysis of the predicted amino acid sequence of 1Sty13 indicated that the N-terminal domain was similar to the y-type subunit, whereas the C-terminal domains were similar to the x-type subunit. Five cysteine residues were found in 1Sty13, which is one less than the published HMW-GS in the St genome. The 1Sty13 protein was purified at a scale sufficient for incorporation into flour for the SDS sedimentation test, which indicated that incorporating 1Sty13 improved dough quality.

Type
Short Communication
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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Footnotes

Mei Yan and Muzi Li contributed equally to this study.

References

Du, XY, Hu, JM, Ma, X, He, JF, Hou, WQ, Guo, J, Bo, CY, Wang, HW, Li, AF and Kong, LR (2019) Molecular characterization and marker development for high molecular weight glutenin subunit 1Dy12** from Yunnan hulled wheat. Molecular Breeding 39: 4.CrossRefGoogle Scholar
Du, XY, Wei, JL, Luo, X, Liu, ZG, Qian, YQ, Zhu, B, Weng, QB and Tang, H (2020) Low-molecular-weight glutenin subunit LMW-N13 improves dough quality of transgenic wheat. Food Chemistry 327: 127048.CrossRefGoogle ScholarPubMed
Garg, M, Kumar, R, Singh, RP and Tsujimoto, H (2014) Development of an Aegilops longissima, substitution line with improved breadmaking quality. Journal of Cereal Science 60: 389396.CrossRefGoogle Scholar
Hou, WQ, Feng, W, Yu, GH, Du, XY and Ren, MJ (2017) Cloning and functional analysis of a novel x-type high-molecular-weight glutenin subunit with altered cysteine residues from Aegilops umbellulata. Crop & Pasture Science 68: 409414.CrossRefGoogle Scholar
Jiang, QT, Wei, YM, Lu, ZX, Liu, T, Wang, JR, Pu, ZE, Lan, ZJ and Zheng, YL (2010) Characterization of a novel variant HMW-glutenin gene from Elymus canadensis. Genes & Genomics 32: 361367.CrossRefGoogle Scholar
Jiang, QT, Zhang, XW, Ma, J, Wei, L, Zhao, S, Zhao, QZ, Qi, PF, Lu, ZX, Zheng, YL and Wei, YM (2014) Characterization of high-molecular weight glutenin subunits from Eremopyrum bonaepartis and identification of a novel variant with unusual high molecular weight and altered cysteine residues. Planta 239: 865875.CrossRefGoogle ScholarPubMed
Ma, CY, Yang, Y, Li, XH, Ge, P, Guo, GF, Subburaj, S, Zeller, FJ, Hsam, SLK and Yan, YM (2013) Molecular cloning and characterization of six novel HMW-GS genes from Aegilops speltoides and Aegilops kotschyi. Plant Breeding 132: 284289.CrossRefGoogle Scholar
Payne, PI, Law, CN and Mudd, EE (1980) Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theoretical and Applied Genetics 58: 113120.CrossRefGoogle Scholar
Rasheed, A, Mahmood, T, Kazi, AG, Ghafoor, A and Mujeeb-Kazi, A (2012) Allelic variation and composition of HMW-GS in advanced lines derived from D-genome synthetic hexaploid/bread wheat (Triticum aestivum L.). Journal of Crop Science and Biotechnology 15: 17.CrossRefGoogle Scholar
Sun, YQ, Pu, ZJ, Dai, SF, Pu, XX, Liu, DC, Wu, BH, Lan, XJ, Wei, YM, Zheng, YL and Yan, ZH (2014) Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus. Development Genes and Evolution 224: 5764.CrossRefGoogle ScholarPubMed
Wan, Y, Wang, D, Shewry, PR and Halford, NG (2002) Isolation and characterization of five novel high-molecular-weight subunit genes from Triticum timopheevi and Aegilops cylindrica. Theoretical and Applied Genetics 104: 828839.CrossRefGoogle ScholarPubMed
Yuan, ZW, Chen, QJ, Zhang, LQ, Yan, ZH, Zheng, YL and Liu, DC (2009) Molecular characterization of two silenced y-type genes for Glu-B1 in Triticum aestivum ssp. yunnanese and ssp. tibetanum. Journal of Integrative Plant Biology 51: 9399.CrossRefGoogle ScholarPubMed
Zhang, ZY, Xie, WG, Zhang, JC, Zhao, XH, Zhao, YQ and Wang, YR (2018) Phenotype and SSR-based estimates of genetic variation between and within two important Elymus species in western and northern China. Genes 9: 147.CrossRefGoogle ScholarPubMed
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