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Variable generation of specific acquired resistance in CBA/Ca mice chronically infected with schistosomiasis mansoni

Published online by Cambridge University Press:  06 April 2009

V. S. Delgado  and
D. J. Mclaren
V. S. Delgado
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
D. J. Mclaren
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
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Summary

Naive CBA/Ca mice and CBA/Ca mice infected 12 weeks previously with 20 normal cercariae of Schistosoma mansoni were challenged percutaneously with isotopically labelled parasites. Challenge worm migration was followed, in 5 separate experiments, by means of compressed organ autoradiography. In three experiments, 43.1 % of challenge parasites did not arrive in the lungs of infected mice on day 6 as compared to 7.7 % in naive controls, thereby indicating that pre-lung sites constitute the first barrier in resistance to reinfection. A further 15% of the challenge worm burden was lost in the lungs or en route to the liver in the immune animals, and portal perfusion revealed that 25.4% of the challenge was lost in the liver. Two other experiments revealed no comparable phases of pre-liver attrition however; instead resistance was only evident at final perfusion on days 28 or 35. These data reveal the variable generation of specific acquired immunity in mice harbouring a chronic schistosome infection and thus clarify current discrepancies in the literature. The results are discussed in relation to documented evidence for the nature of specific and non-specific immune mechanisms reported to operate at different sites in the infection model of schistosomiasis mansoni.

Keywords

Schistosoma mansonichronic infection resistanceimmune mechanismsautoradiographic tracking.
Type
Research Article
Information
Parasitology , Volume 99 , Issue 3 , December 1989 , pp. 357 - 364
Copyright
Copyright © Cambridge University Press 1989

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