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Use of larval, parasitic female and egg antigens from Strongyloides venezuelensis to detect parasite-specific IgG and immune complexes in immunodiagnosis of human strongyloidiasis

Published online by Cambridge University Press:  06 February 2012

A. L. R. GONÇALVES ,
D. S. NUNES ,
M. R. F. GONÇALVES-PIRES ,
M. T. UETA  and
J. M. COSTA-CRUZ
A. L. R. GONÇALVES
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
D. S. NUNES
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
M. R. F. GONÇALVES-PIRES
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
M. T. UETA
Affiliation:
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil
J. M. COSTA-CRUZ*
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
*
*Corresponding author. Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil. Avenida Pará 1720, 38400-902, Uberlândia, MG, Brasil. Tel: +55 34 3218 2187. Fax: +55 34 3118 2333. E-mail: costacruz@ufu.br
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Summary

The aim of this study was to use larval, parasitic female and egg antigens from Strongyloides venezuelensis to detect parasite-specific IgG and immune complexes in human serum samples by enzyme-linked immunosorbent assay (ELISA). In total, 95 serum samples were analysed, consisting of 30 patients harbouring S. stercoralis larvae, 30 healthy subjects and 35 patients with other parasites. Sensitivity, specificity and diagnostic efficiency were calculated. A significant statistical difference was found in the detection of immune complexes and antibodies in patients harbouring S. stercoralis larvae from larval and eggs antigens, with higher positivity using larval antigen. The larval antigen showed the highest values for sensitivity, specificity and diagnostic efficiency in ELISA from detection of immune complexes. For the first time we used IgG anti-larvae, IgG anti-parasitic females or IgG anti-eggs for immune complex detection. We concluded that the association of antibody and immune complex detection could be used in the diagnosis of human strongyloidiasis.

Keywords

antigendiagnosisimmune complexstrongyloidiasis
Type
Research Article
Information
Parasitology , Volume 139 , Issue 7 , June 2012 , pp. 956 - 961
Copyright
Copyright © Cambridge University Press 2012

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