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Trichinella spiralis newborn larvae: characterization of a stage specific serine proteinase expression, NBL1, using monoclonal antibodies

Published online by Cambridge University Press:  19 January 2015

YONG YANG ,
SANDRINE A. LACOUR ,
VÉRONIQUE LAINÉ-PRADE ,
NICOLAS VERSILLÉ ,
AURÉLIE GRASSET-CHEVILLOT ,
SHUANG FENG ,
MING YUAN LIU ,
PASCAL BOIREAU  and
ISABELLE VALLÉE
YONG YANG
Affiliation:
Animal Health Laboratory, Université Paris-Est, Anses, ENVA, JRU BIPAR, Maisons-Alfort, France Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, People's Republic of China
SANDRINE A. LACOUR
Affiliation:
Animal Health Laboratory, Université Paris-Est, Anses, ENVA, JRU BIPAR, Maisons-Alfort, France
VÉRONIQUE LAINÉ-PRADE
Affiliation:
Animal Health Laboratory, Université Paris-Est, Anses, ENVA, JRU BIPAR, Maisons-Alfort, France
NICOLAS VERSILLÉ
Affiliation:
Animal Health Laboratory, Université Paris-Est, Anses, ENVA, JRU BIPAR, Maisons-Alfort, France
AURÉLIE GRASSET-CHEVILLOT
Affiliation:
Animal Health Laboratory, Université Paris-Est, Anses, ENVA, JRU BIPAR, Maisons-Alfort, France
SHUANG FENG
Affiliation:
Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, People's Republic of China
MING YUAN LIU*
Affiliation:
Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, People's Republic of China
PASCAL BOIREAU
Affiliation:
Animal Health Laboratory, Université Paris-Est, Anses, ENVA, JRU BIPAR, Maisons-Alfort, France
ISABELLE VALLÉE*
Affiliation:
Animal Health Laboratory, Université Paris-Est, Anses, ENVA, JRU BIPAR, Maisons-Alfort, France
*
*Corresponding authors. Animal Health Laboratory, JRU BIPAR, Anses Maisons-Alfort, 14 rue Pierre et Marie Curie, 94700 Maisons-Alfort, France. E-mail: isabelle.vallee@anses.fr; Key Laboratory for Zoonosis Research, Institute of Zoonosis, Jilin University, 5333 Xian Road, 130062 Changchun, People's Republic of China. E-mail: liumy@jlu.edu.cn
*Corresponding authors. Animal Health Laboratory, JRU BIPAR, Anses Maisons-Alfort, 14 rue Pierre et Marie Curie, 94700 Maisons-Alfort, France. E-mail: isabelle.vallee@anses.fr; Key Laboratory for Zoonosis Research, Institute of Zoonosis, Jilin University, 5333 Xian Road, 130062 Changchun, People's Republic of China. E-mail: liumy@jlu.edu.cn
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Summary

Trichinella spiralis is an intracellular parasitic nematode of mammalian skeletal muscle, causing a serious zoonotic disease in humans and showing a high economic impact mainly in pig breeding. Serine proteinases of T. spiralis play important roles in the host–parasite interactions mediating host invasion. In this study, we have focused on newborn larvae (NBL-1), the first identified serine proteinase from the NBL stage of T. spiralis. Five monoclonal antibodies (mAbs) directed against the C-terminal part of NBL1, were produced. These mAbs were IgG1κ isotype and specifically recognized as a common motif of 10 amino acids (PSSGSRPTYP). Selected mAbs were further characterized using antigens from various developmental stages of T. spiralis. Western blot revealed that selected mAbs reacted with the native NBL1 at Mr 50 kDa in the adult and NBL mixed antigens and NBL stage alone. Indirect immunofluorescence analysis revealed that selected mAbs intensely stained only the embryos within the gravid females and the NBL. Thus, the produced mAbs are useful tools for the characterization of NBL1 as a major antigen of Trichinella involved in the invasion of the host but also for the development of new serological tests with an early detection of T. spiralis infection.

Keywords

Trichinella spiralisserine proteinaseimmunodominant antigenmonoclonal antibodies
Type
Research Article
Information
Parasitology , Volume 142 , Issue 6 , May 2015 , pp. 783 - 790
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Copyright
Copyright © Cambridge University Press 2015 

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