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Toxoplasma gondii-structure variations of the antigen P30

Published online by Cambridge University Press:  06 April 2009

A. Bonhomme ,
C. Thirion ,
F. Boulanger ,
F. Charton ,
H. Burlet ,
J. M. Pinon  and
A. J. P. Alix
A. Bonhomme
Affiliation:
Laboratoire de Parasitologie, Université (URCA), INSERM U.314, C. H. U. Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cédex, France
C. Thirion
Affiliation:
Laboratoire de Spectroscopie et Structures Biomoléculaires, Université (URCA), INSERM U.314, C. H. U. Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cédex, France
F. Boulanger
Affiliation:
Laboratoire de Parasitologie, Université (URCA), INSERM U.314, C. H. U. Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cédex, France
F. Charton
Affiliation:
Laboratoire de Spectroscopie et Structures Biomoléculaires, Université (URCA), INSERM U.314, C. H. U. Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cédex, France
H. Burlet
Affiliation:
Laboratoire de Parasitologie, Université (URCA), INSERM U.314, C. H. U. Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cédex, France
J. M. Pinon
Affiliation:
Laboratoire de Parasitologie, Université (URCA), INSERM U.314, C. H. U. Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cédex, France
A. J. P. Alix
Affiliation:
Laboratoire de Spectroscopie et Structures Biomoléculaires, Université (URCA), INSERM U.314, C. H. U. Hôpital Maison Blanche, 45 rue Cognacq Jay, 51092 Reims Cédex, France
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Summary

The major surface immunodominant antigen (P30) of Toxoplasma gondii was purified by two methods (i) SDS–PAGE and (ii) immunoaffinity chromatography. The secondary elements within this protein were assessed by circular dichroism and spectra obtained were compared to those proposed by Manavalan & Johnson (1983). The results allowed us to determine an all β protein status for this antigen. This experimental result was in agreement with the predicted secondary structures deduced from the P30 primary sequence. Modifications in conformation according to pH and temperature were recorded without any change in immunoactivity. The epitope, which was always recognized by a monoclonal antibody against P30, could be a linear epitope.

Keywords

Toxoplasma gondiiantigen P30circular dichroismmolecular conformation
Type
Research Article
Information
Parasitology , Volume 108 , Issue 3 , April 1994 , pp. 281 - 287
Copyright
Copyright © Cambridge University Press 1994

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References

REFERENCES

Araujo, F. G. & Remington, J. S. (1984). Partially purified antigen preparations of Toxoplasma gondii protect against lethal infection in mice. Infection and Immunity 45, 122–6.CrossRefGoogle ScholarPubMed
Bonhomme, A., Boulanger, F., Bharadwaj, L. M., Puygauthier-Toubas, D., Bonhomme, P., Pluot, M. & Pinon, J. M. (1990). Toxoplasma gondii: immunocytochemistry of four immunodominant antigens with monoclonal antibodies. Experimental Parasitology 71, 439–51.CrossRefGoogle ScholarPubMed
Boulanger, F., Bonhomme, A., Lebonvallet, S., Bonnet, N., Pluot, M. & Pinon, J. M. (1992). Quantitative immunolocalization of four immunodominant antigens of Toxoplasma gondii. Biology of the Cell 73, 89–97.CrossRefGoogle Scholar
Burg, J. L., Perelman, D., Kasper, L. H., Ware, P. L. & Boothroyd, J. C. (1988). Molecular analysis of the gene coding the major surface antigen of Toxoplasma gondii. Journal of Immunology 141, 3584–91.CrossRefGoogle ScholarPubMed
Edman, P. & Begg, G. (1967). A protein sequensator. European Journal of Biochemistry 1, 80–91.CrossRefGoogle Scholar
Johnson, A. M., McDonald, P. J. & Neoh, S. H. (1983). Monoclonal antibodies to Toxoplasma gondii cell membrane surface antigens protect mice from toxoplasmosis. Journal of Protozoology 30, 351–6.CrossRefGoogle ScholarPubMed
Kasper, L. H., Crabb, J. H. & Pfefferkorn, E. R. (1983). Purification of a major membrane protein of Toxoplasma gondii by immunoadsorption with a monoclonal antibody. Journal of Immunology 130, 2407–14.CrossRefGoogle Scholar
Kasper, L. H., Currie, K. M. & Bradley, M. S. (1985). An unexpected response to vaccination with a purified major membrane tachyzoite antigen (P30) of Toxoplasma gondii. Journal of Immunology 134, 3426–31.CrossRefGoogle ScholarPubMed
Khan, I. A., Smith, K. A. & Kasper, L. H. (1988). Induction of antigen specific parasiticidal cytotoxic T cell splenocytes by a major membrane protein (P30) of Toxoplasma gondii. Journal of Immunology 141, 3600–5.CrossRefGoogle Scholar
Manavalan, P. & Johnson, W. C. (1983). Sensitivity of circular dichroism to protein tertiary class. Nature, London 305, 831–2.CrossRefGoogle Scholar
Nagel, S. D. & Boothroyd, J. C. (1989). The major surface antigen P30 of Toxoplasma gondii is anchored by a glycolipid. Journal of Biological Chemistry 264, 5569–74.CrossRefGoogle ScholarPubMed
Pinon, J. M., Thoannes, H. & Gruson, N. (1985). An Enzyme-Linked Immuno-Filtration Assay used to compare infant and maternal antibody profiles in toxoplasmosis. Journal of Immunological Methods 77, 15–23.CrossRefGoogle ScholarPubMed
Rodriguez, C., Afchain, D., Capron, A., Dissous, C. & Santoro, F. (1985). Major surface protein of Toxoplasma gondii (P30) contains an immunodominant region with repetitive epitopes. European Journal of Immunology 15, 747–9.CrossRefGoogle ScholarPubMed
Santoro, F., Afchain, D., Pierce, R., Cesbron, Y., Ovlaque, G. & Capron, A. (1985). Serodiagnosis of Toxoplasma infection using a purified parasite protein (P30). Clinical and Experimental Immunology 62, 262–9.Google ScholarPubMed
Santoro, F., Charif, H. & Capron, A. (1986). The immunodominant epitope of the major membrane tachyzoite protein (P30) of Toxoplasma gondii. Parasite Immunology 8, 631–9.CrossRefGoogle ScholarPubMed
Sibley, L. D. & Sharma, S. D. (1987). Ultrastructural localization of an intracellular Toxoplasma protein that induces protection in mice. Infection and Immunity 55, 2137–41.CrossRefGoogle ScholarPubMed
Tomavo, S., Schwartz, R. T. & Dubremetz, J. F. (1989). Evidence for glycosyl-phosphatidyl inositol anchoring of Toxoplasma gondii major surface antigens. Molecular and Cellular Biology 9, 4576–80.Google Scholar
Towbin, H., Staehelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gel to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, USA 76, 4350–4.CrossRefGoogle ScholarPubMed