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The specificity of purine base and nucleoside uptake in promastigotes of Leishmania braziliensis panamensis

Published online by Cambridge University Press:  06 April 2009

B. D. Hansen ,
J. Perez-Arbelo ,
J. F. Walkony  and
L. D. Hendricks
B. D. Hansen
Affiliation:
Division of Biochemistry, Walter Reed Army Institute of Research, Washington, D.C. 20012
J. Perez-Arbelo
Affiliation:
Division of Biochemistry, Walter Reed Army Institute of Research, Washington, D.C. 20012
J. F. Walkony
Affiliation:
Division of Biochemistry, Walter Reed Army Institute of Research, Washington, D.C. 20012
L. D. Hendricks
Affiliation:
Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Washington, D.C. 20012
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Summary

Promastigotes of Leishmania braziliensis panamensis absorbed the purines adenine, hypoxanthine, adenosine and inosine by a combination of diffusion and mediated components. When the uptake rates for these substrates were corrected for diffusion and compared, the purine bases adenine and hypoxanthine were transported at a significantly slower rate than the purine nucleosides adenosine and inosine. Competitive interactions among those purines tested confirmed the presence of mediated and diffusion components and suggested that three transport loci may be operating (Fig. 6). The first transport locus, designated Locus 1, transported inosine, Locus 2, the purine bases hypoxanthine and adenine and Locus 3, adenosine. In addition, adenine and hypoxanthine inhibited the uptake of one another competitively. A comparison of Ki values derived from double reciprocal plots of labelled hypoxanthine and adenine uptake in the presence of the unlabelled substrates as inhibitors suggested that adenine has a greater affinity for the transport locus.

Type
Research Article
Information
Parasitology , Volume 85 , Issue 2 , October 1982 , pp. 271 - 282
Copyright
Copyright © Cambridge University Press 1982

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