Hostname: page-component-8448b6f56d-dnltx Total loading time: 0 Render date: 2024-04-19T15:52:23.227Z Has data issue: false hasContentIssue false
  • English
  • Français

Reappraisal of the guinea-pig as an experimental host for studies of schistosomiasis mansoni

Published online by Cambridge University Press:  06 April 2009

E. J. Pearce  and
Diane J. McLaren
E. J. Pearce
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
Diane J. McLaren
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
Get access Rights & Permissions [Opens in a new window]

Summary

The guinea-pig has been reassessed as a potential laboratory host for Schistosoma mansoni. Twenty-six per cent of an infective cercarial population survive to maturity in this rodent and there are no gross fluctuations in worm burden subsequent to pairing of male and female parasites. Five day, 4-week and 6-week-old schistosomes grown in guinea-pigs have topographical features that are identical to those exhibited by similarly aged mouse worms, but different from those exhibited by rat worms. Schistosome eggs are never detected in the faeces of infected guinea-pigs, but they can be observed in the pulmonary, hepatic and intestinal tissues. Only 55% of the eggs that can be recovered from the intestinal tissues are viable, and some of these can be hatched to release miracidia that penetrate the intermediate snail host. Cercariae are sometimes liberated from infected snails, but in insufficient numbers to permit infection of naive guinea-pigs. The schistosome cycle cannot, therefore, be completed in this host. Collaterals are sometimes observed in the vasculature serving the rectum and kidneys of infected guinea-pigs and the fact that schistosome eggs are deposited in the lungs of these animals indicates that portal systemic anastomosis is a feature of the model. Pathology associated with cercarial invasion or egg deposition is not dissimilar to that described for other laboratory animals infected with S. mansoni, except that basophils participate in the inflammatory response observed in the skin and intestine.

Type
Research Article
Information
Parasitology , Volume 87 , Issue 3 , December 1983 , pp. 455 - 464
Copyright
Copyright © Cambridge University Press 1983

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Altman, P. L. & Katz, D. D. (1979). Inbred and genetically defined strains of laboratory animals. Part 2. Federation of American Societies for Experimental Biology, Bethesda, Maryland. pp. 505–63.Google Scholar
Bayer, . (1968). The Microscopic Diagnosis of Tropical Diseases. Bayer Pharmaceuticals Germany.Google Scholar
Bentley, A. G., Carlisle, A. S. & Phillips, S. M. (1981). Ultrastructural analysis of the cellular response to Schistosoma mansoni. II. Inflammatory response in rodent skin. American Journal of Tropical Medicine and Hygiene 30, 815–24.CrossRefGoogle ScholarPubMed
Chen, P. & Dean, D. A. (1977). Immune response of guinea pigs to Schistosoma mansoni. II. In vitro blastogenesis and macrophage migration inhibition factor production in response to cercarial, adult worm and egg antigens. American Journal of Tropical Medicine and Hygiene 26, 963–9.CrossRefGoogle Scholar
Colley, D. G., Magalhães-Filho, A. & Coelho, R. B. (1972). Immunopathology of dermal reactions induced by Schistosoma mansoni cercariae and cercarial extract. American Journal of Tropical Medicine and Hygiene 21, 558–68.CrossRefGoogle ScholarPubMed
Clegg, J. A. (1965). In vitro cultivation of Schistosoma mansoni. Experimental Parasitology 16, 133–47.CrossRefGoogle ScholarPubMed
Doenhoff, M., Musallam, R., Bain, J. & McGregor, A. (1978). Studies on the host parasite relationship in Schistosoma mansoni infected mice. The immunological dependence of parasite egg excretion. Immunology 35, 771–8.Google ScholarPubMed
Doenhoff, M., Bickle, Q., Long, E., Bain, J. & McGregor, A. (1978). Factors affecting the acquistion of resistance against Schistosoma mansoni in the mouse. I. Demonstration of resistance to reinfection using a model system that involves perfusion of mice within three weeks of challenge. Journal of Helminthology 52, 173–86.CrossRefGoogle Scholar
Galli, S. J., Colvin, R. B., Verderber, E., Galli, A. S., Monahan, R., Dvorak, A. M. & Dvorak, H. F. (1978). Preparation of a rabbit anti-guinea pig basophil serum: in vitro and in vivo characterization. Journal of Immunology 121, 1157–66.CrossRefGoogle ScholarPubMed
Gleich, G. J., Loegering, D. A. & Olson, G. M. (1975). Reactivity of rabbit antiserum to guinea pig eosinophils. Journal of Immunology 115, 950–4.CrossRefGoogle ScholarPubMed
Hockley, D. J. & McLaren, D. J. (1973). Schistosoma mansoni: changes in the outer membrane of the tegument during development from cercariae to adult worms. International Journal for Parasitology 3, 13–25.CrossRefGoogle Scholar
Hsü, S. Y., Hsü, H. F. & Lust, G. L. (1972). Possible manifestations of immunological tolerance in lesions elicited by schistosome eggs in chronic stages of infection. Federation Proceedings 31, 774.Google Scholar
Hsü, S. Y. Li, Hsü, H. F., Lust, G. L., Davis, J. R. & Eveland, L. K. (1973). Comparative studies on the lesions caused by eggs of Schistosoma japonicum and Schistosoma mansoni in the liver of hamsters, guinea-pigs and albino rats. Annals of Tropical Medicine and Parasitology 67, 349–56.CrossRefGoogle ScholarPubMed
Incani, R. N. (1980). Immunity to Schistosoma mansoni: in vitro and in vivo studies on the role of the neutrophil in schistosomular killing. Ph.D thesis, Brunel University, Uxbridge.Google Scholar
Jones, W. O., Rothwell, T. L. W., Dineen, J. K. & Griffiths, D. A. (1974). Studies on the role of histamine and 5-hydroxytryptamine in immunity against the nematode Trichostrongylus colubriformis. II. Amine levels in the intestine of infected guinea pigs. International Archives of Allergy and Applied Immunology 46, 14–27.CrossRefGoogle ScholarPubMed
La Grange, E. & Scheeqmans, G. (1949). La bilhariose experimental du cobaye. Comptes rendus des séances de la sociétè de biologie de ses filiales 143, 1396–9.Google Scholar
Lichtenberg, F. von, Sher, A., Gibbons, N. & Doughty, B. L. (1976). Eosinophil-enriched inflammatory response to schistosomula in the skin of mice immune to Schistosoma mansoni. American Journal of Pathology 84, 479–500.Google ScholarPubMed
McLaren, D. J. (1980). Schistosoma mansoni. The Parasite Surface in Relation to Host Immunity, vol. 1, (ed. Brown, K. N.) Tropical Medicine Research Studies Press, Chichester: John Wiley.Google Scholar
Michaels, R. M. (1970). Schistosoma mansoni: alteration in ovipositing capacity by transplanting between heterologous hosts. Experimental Parasitology 27, 217–28.CrossRefGoogle ScholarPubMed
Moore, D. V., Yolles, T. K. & Meleney, H. E. (1949). A comparison of common laboratory animals as experimental hosts for Schistosoma mansoni. Journal of Parasitology 35, 156–70.CrossRefGoogle ScholarPubMed
Savage, A. M. & Colley, D. G. (1980). The eosinophil in the inflammatory response to cercarial challenge of sensitized and chronically infected CBA/J mice. American Journal of Tropical Medicine and Hygiene 29, 1268–78.CrossRefGoogle ScholarPubMed
Senft, A. W., Gibler, W. B. & Knopf, P. M. (1978). Scanning electron microscope observations on tegument maturation in Schistosoma mansoni from permissive and non-permissive hosts. American Journal of Tropical Medicine and Hygiene 27, 258–66.CrossRefGoogle ScholarPubMed
Smithers, S. R. (1982). The demonstration of immunity to Schistosoma mansoni in the mouse and its correlation with in vitro findings. Pontificiae Academiae Scientarium Scripta Varia 47, 133–43.Google Scholar
Smithers, S. R., McLaben, D. J. & Ramalho-Pinto, F. J. (1977). Immunity to schistosomes: the target. American Journal of Tropical Medicine and Hygiene 26, 11–19.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1967). Resistance to experimental infection with Schistosoma mansoni in rhesus monkeys induced by the transfer of adult worms. Transactions of the Royal Society of Tropical Medicine and Hygiene 61, 517–33.CrossRefGoogle ScholarPubMed
Stirewalt, M. A., Kuntz, R. E. & Evans, A. S. (1951). The relative susceptibilities of the commonly used laboratory mammals to infection with Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 31, 57–82.CrossRefGoogle ScholarPubMed
Thompson, J. H. (1954). Host parasite relationships of Schistosoma mansoni. Experimental Parasitology 3, 140–60.CrossRefGoogle ScholarPubMed
Warren, K. S. & Peters, P. A. (1967). Comparison of penetration and maturation of Schistosoma mansoni in the hamster, mouse, guinea pig, rabbit and rat. American Journal of Tropical Medicine and Hygiene 16, 718–22.CrossRefGoogle Scholar
Weinstock, J. V. & Boros, D. L. (1983). Organ-dependent differences in composition and function observed in hepatic and intestinal granulomas isolated from mice with schistosomiasis mansoni. Journal of Immunology 130, 418–22.CrossRefGoogle ScholarPubMed