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Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls

  • M. N. WASS (a1), R. STANWAY (a2) (a3), A. M. BLAGBOROUGH (a2), K. LAL (a2), J. H. PRIETO (a4), D. RAINE (a2), M. J. E. STERNBERG (a1), A. M. TALMAN (a2) (a5), F. TOMLEY (a6), J. YATES (a4) and R. E. SINDEN (a2)...


Here we discuss proteomic analyses of whole cell preparations of the mosquito stages of malaria parasite development (i.e. gametocytes, microgamete, ookinete, oocyst and sporozoite) of Plasmodium berghei. We also include critiques of the proteomes of two cell fractions from the purified ookinete, namely the micronemes and cell surface. Whereas we summarise key biological interpretations of the data, we also try to identify key methodological constraints we have met, only some of which we were able to resolve. Recognising the need to translate the potential of current genome sequencing into functional understanding, we report our efforts to develop more powerful combinations of methods for the in silico prediction of protein function and location. We have applied this analysis to the proteome of the male gamete, a cell whose very simple structural organisation facilitated interpretation of data. Some of the in silico predictions made have now been supported by ongoing protein tagging and genetic knockout studies. We hope this discussion may assist future studies.

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Abromaitis, S. and Stephens, R. S. (2009). Attachment and entry of Chlamydia have distinct requirements for host protein disulfide isomerase. PLoS Pathogens Apr;5(4), e1000357. Epub 2009 Apr 3.
Altschul, S. F., Gish, W., Miller, W., Myers, E. W. and Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology 215, 403410.
Ashburner, M., Ball, C. A., Blake, J. A., Botstein, D., Butler, H., Cherry, J. M., Davis, A. P., Dolinski, K., Dwight, S. S., Eppig, J. T., Harris, M. A., Hill, D. P., Issel-Tarver, L., Kasarskis, A., Lewis, S., Matese, J. C., Richardson, J. E., Ringwald, M., Rubin, G. M. and Sherlock, G. (2000). Gene ontology, tool for the unification of biology. The Gene Ontology Consortium. Nature Genetics 25, 2529.
Bairoch, A. (1999). The ENZYME data bank in 1999. Nucleic Acids Research 27, 310311.
Bernsel, A., Viklund, H., Falk, J., Lindahl, E., von Heijne, G. and Elofsson, A. (2008). Prediction of membrane-protein topology from first principles. Proceedings of the National Academy of Sciences, USA 105, 71777181.
Bernsel, A., Viklund, H., Hennerdal, A. and Elofsson, A. (2009). TOPCONS, consensus prediction of membrane protein topology. Nucleic Acids Research 37(Web Server issue), W4658. doi:10.1093/nar/gkp363
Bi, S., Hong, P. W., Lee, B. and Baum, L. G. (2011). Galectin-9 binding to cell surface protein disulfide isomerase regulates the redox environment to enhance T-cell migration and HIV entry. Proceedings of the National Academy of Sciences, USA 108, 1065010655.
Blair, P. L. and Carucci, D. J. (2005). Functional proteome and expression analysis of sporozoites and hepatic stages of malaria development. Current Topics in Microbiology and Immunology 295, 417438.
Bozdech, Z., Zhu, J., Joachimiak, M. P., Cohen, F. E., Pulliam, B. and DeRisi, J. L. (2003). Expression profiling of the schizont and trophozoite stages of Plasmodium falciparum with a long-oligonucleotide microarray. Genome Biology 4, R9. Epub 2003 Jan 31.
Briesemeister, S., Blum, T., Brady, S., Lam, Y., Kohlbacher, O. and Shatkay, H. (2009). SherLoc2, a high-accuracy hybrid method for predicting subcellular localization of proteins. Journal of Proteome Research 8, 53635366.
Carlton, J. M., Angiuoli, S. V., Suh, B. B., Kooij, T., Pertea, M., Silva, J. C., Ermolaeva, M. D., Allen, J. E., Selengut, J. D., Koo, H. L., Peterson, J. D., Pop, M., Kosack, D. S., Shumway, M. F., Bidwell, S. L., Shallom, S. J., Van Aken, S. E., Riedmuller, S. B., Feldylum, T. V., Cho, J. K., Quackenbush, J., Sedegah, M., Shoalbi, A., Cummings, L. M., Florens, L., Yates, J. R., Raine, D. J., Sinden, R. E., Haris, M. A., Cunningham, D. A., Preiser, P. R., Bergman, L. W., Vaidya, A., Van Lin, L. H., Janse, C. J., Waters, A. P., Smith, H. O., White, O. R., Salzberg, J., Venter, C., Fraser, C. M., Hoffman, S. L., Gardner, M. J. and Carucci, J. (2002). Genome sequence and comparative analysis of the model rodent malaria parasite Plasmodium yoelii yoelii. Nature 419, 512519.
Carucci, D. J., Yates, J. R. III and Florens, L. (2002). Exploring the proteome of Plasmodium. International Journal for Parasitology 32, 15391542.
Chou, K.-C., and Shen, H.-B. (2007). Euk-mPLoc, a fusion classifier for large-scale eukaryotic protein subcellular location prediction by incorporating multiple sites. Journal of Proteome Research 6, 17281734.
Cociancich, S. O., Park, S. S., Fidock, D. A. and Shahabuddin, M. (1999). Vesicular ATPase-overexpressing cells determine the distribution of malaria parasite oocysts on the midguts of mosquitoes. Journal of Biological Chemistry 274, 1265012655.
Creasey, A. M., Ranford Cartwright, L. C., Moore, D. J., Williamson, D. H., Wilson, R. J. M., Walliker, D. and Carter, R. (1993). Uniparental inheritance of the mitochondrial gene cytochrome-b in Plasmodium falciparum. Current Genetics 23, 360364.
Deligianni, E., Morgan, R. N., Bertuccini, L., Kooij, T. W., Laforge, A., Nahar, C., Poulakakis, N., Schüler, H., Louis, C., Matuschewski, A. and Siden-Kiamos, I. (2011). Critical role for a stage-specific actin in male exflagellation of the malaria parasite. Cellular Microbiology 13, 17141730.
Doolan, D. L., Southwood, S., Freilich, D. A., Sidney, J., Graber, N. L., Shatney, L., Bebris, L., Florens, L., Dobano, C., Witney, A. A., Appella, E., Hoffman, S. L., Yates, J. R., Carucci, D. J. and Sette, A. (2003). Identification of Plasmodium falciparum antigens by antigenic analysis of genomic and proteomic data. Proceedings of the National Academy of Sciences, USA 100, 99529957.
Emanuelsson, O., Brunak, S., von Heijne, G. and Nielsen, H. (2007). Locating proteins in the cell using TargetP, SignalP and related tools. Nature Protocols 2, 953971.
Florens, L., Washburn, M. P., Raine, J. D., Anthony, R. M., Grainger, M., Haynes, J. D., Moch, J. K., Muster, N., Sacci, J. B., Tabb, D. L., Witney, A. A., Wolters, D., Wu, Y., Gardner, M. J., Holder, A. A., Sinden, R. E., Yates, J. R. and Carucci, D. J. (2002). A proteomic view of the Plasmodium falciparum life cycle. Nature 419, 520526.
Forslund, K., and Sonnhammer, E. L. L. (2008). Predicting protein function from domain content. Bioinformatics 24, 16811687.
Foth, B. J., Zhang, N., Chaal, B. K., Sze, S. K., Preiser, P. R. and Bozdech, Z. (2011). Quantitative time-course profiling of parasite and host cell proteins in the human malaria parasite Plasmodium falciparum. Molecular and Cellular Proteomics 10, M110.006411. Epub 2011 May 10.
Garg, A. and Raghava, G. (2008). ESLpred2, improved method for predicting subcellular localization of eukaryotic proteins. BMC Bioinformatics 9, 503.
Gass, R. F. (1977). Influences of blood digestion on the development of Plasmodium gallinaceum (Brumpt). in the midgut of Aedes aegypti (L.). Acta Tropica 34, 127140.
Gass, R. F. and Yeates, R. A. (1979). In vitro damaged of cultured ookinetes of Plasmodium gallinaceum by digestive proteinases from susceptible Aedes aegypti. Acta Tropica 36, 243252.
Gomes-Santos, C. S., Braks, J., Prudencio, M., Carret, C., Gomes, A. R., Pain, A., Feltwell, T., Khan, S., Waters, A., Janse, C., Mair, G. R. and Mota, M. M. (2011). Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio. PLoS Pathogens 7, e1002046. Epub 2011 May 19.
Hadley, T. J. and Miller, L. H. (1988). Invasion of erythrocytes by malaria parasites, Erythrocyte ligands and parasite receptors. Progress in Allergy 41, 4971.
Hall, N., Karras, M., Raine, J. D., Carlton, J. M., Kooij, T. W. J., Berriman, M., Florens, L., Janssen, C. S., Pain, A., Christophides, G. K., James, K., Rutherford, K., Harris, B., Harris, D. B., Churcher, C., Quail, M. A., Ormond, D., Doggett, J., Trueman, H. E., Mendoza, J., Bidwell, S. L., Rajandream, M.-A., Carucci, D. J., Yates, I. J. R., Kafatos, F. C., Janse, C. J., Barrell, B., Turner, C. M. R., Waters, A. P. and Sinden, R. E. (2005). A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses. Science 307, 8286.
Han, Y. S. and Barillas-Mury, C. (2002). Implications of time bomb model of ookinete invasion of midgut cells. Insect Biochemistry and Molecular Biology 32, 13111316.
Hawkins, T., Luban, S. and Kihara, D. (2006). Enhanced automated function prediction using distantly related sequences and contextual association by PFP. Protein Science 15, 15501556.
Hernandez-Romano, J., Rodriguez, M. H., Pando, V., Torres-Monzom, J. A., Alvarado-Delgado, A., Lecona Valera, A. N., Ramos, R. A., Martinez-Barnetche, J. and Rodriguez, M. C. (2011). Conserved peptide sequences bind to actin and enolase on the surface of Plasmodium berghei ookinetes. Parasitology 138, 13411353.
Horton, P., Park, K.-J., Obayashi, T., Fujita, N., Harada, H., Adams-Collier, C. J. and Nakai, K. (2007). WoLF PSORT, protein localization predictor. Nucleic Acids Research 35, (Web Server issue), W585587.
Hunter, S., Apweiler, R., Attwood, T. K., Bairoch, A., Bateman, A., Binns, D., Bork, P., Das, U., Daugherty, L., Duquenne, L., Finn, R. D., Gough, J., Haft, D., Hulo, N., Kahn, D., Kelly, E., Laugraud, A., Letunic, I., Lonsdale, D., Lopez, R., Madera, M., Maslen, J., McAnulla, C., McDowall, J., Mistry, J., Mitchell, A., Mulder, N., Natale, D., Orengo, C., Quinn, A. F., Selengut, J. D., Sigrist, C. J., Thimma, M., Thomas, P. D., Valentin, F., Wilson, D., Wu, C. H. and Yeats, C. (2009). InterPro, the integrative protein signature database. Nucleic Acids Research 37, D211–D215.
Kappe, S. H. I., Gardner, M. J., Brown, S. M., Ross, J., Matuschewski, K., Ribeiro, J. M., Adams, J. H., Quackenbush, J., Cho, J., Carucci, D. J., Hoffman, S. L. and Nussenzweig, V. (2001). Exploring the transcriptome of the malaria sporozoite stage. Proceedings of the National Academy of Sciences, USA 98, 98959900.
Kelley, L. A. and Sternberg, M. J. (2009). Protein structure prediction on the Web, a case study using the Phyre server. Nature Protocols 4, 363371.
Khan, S. M., Franke-Fayard, B., Mair, G. R., Lasonder, E., Janse, C. J., Mann, M. and Waters, A. P. (2005). Proteome analysis of separated male and female gametocytes reveals novel sex-specific Plasmodium biology. Cell 121, 675687.
Kooij, T. W. A., Janse, C. J. and Waters, A. P. (2006). Plasmodium post-genomics, better the bug you know? Nature Reviews Microbiology 4, 344357.
Krogh, A., Larsson, B., von Heijne, G. and Sonnhammer, E. L. (2001). Predicting transmembrane protein topology with a hidden Markov model, application to complete genomes. Journal of Molecular Biology 305, 567580.5.
Krotoski, W. A. (1985). Discovery of the hypnozoite and a new theory of malarial relapse. Transactions of the Royal Society of tropical Medicine and Hygiene 79, 111.
Lal, K., Prieto, J. H., Bromley, E., Sanderson, S. J., Yates, J. R., Wastling, J. M., Tomley, F. M. and Sinden, R. E. (2009). Characterisation of Plasmodium invasive organelles; an ookinete microneme proteome. Proteomics 9, 11421151.
Lasonder, E., Ishihama, Y., Andersen, J. S., Vermunt, A. M., Pain, A., Sauerwein, R. W., Eling, W. M., Hall, N., Waters, A. P., Stunnenberg, H. G. and Mann, M. (2002). Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry. Nature 419, 537542.
Lasonder, E., Janse, C. J., van Gemert, G.-J., Mair, G. R., Vermunt, A. M., Douradinha, B. G., van Noort, V., Huynen, M. A., Luty, A. J., Kroeze, H., Khan, S. M., Sauerwein, R. W., Waters, A. P., Mann, M. and Stunnenberg, H. G. (2008). Proteomic profiling of Plasmodium sporozoite maturation identifies new proteins essential for parasite development and infectivity. PLoS Pathogens 4, e1000195. Epub 2008 Oct 31.
Lobley, A. E., Nugent, T., Orengo, C. A. and Jones, D. T. (2008). FFPred, an integrated feature-based function prediction server for vertebrate proteomes. Nucleic acids research 36, (Web Server issue), W297302.
Mair, G., Braks, J. A. M., Garver, L. S., Wiegant, J. C. A. G., Hall, N., Dirks, R. W., Khan, S. M., Dimopoulos, G., Janse, C. J. and Waters, A. P. (2006). Regulation of sexual development of Plasmodium by translational repression. Science 313, 667669.
Martin, R., Ginsburg, H. and Kirk, K. (2009). Membrane transport proteins of the malaria parasite. Molecular Microbiology 74, 518528.
Meek, B., Back, J. W., Klaren, V. N., Speijer, D. and Peek, R. (2002). Conserved regions of protein disulfide isomerase are targeted by natural IgA antibodies in humans. International Immunology 11, 12911301.
Mulder, N. J., Apweiler, R., Attwood, T. K., Bairoch, A., Bateman, A., Binns, D., Bork, P., Buillard, V., Cerutti, L., Copley, R., Courcelle, E., Das, U., Daugherty, L., Dibley, M., Finn, R., Fleischmann, W., Gough, J., Haft, D., Hulo, N., Hunter, S., Kahn, D., Kanapin, A., Kejariwal, A., Labarga, A., Langendijk-Genevaux, P. S., Lonsdale, D., Lopez, R., Letunic, I., Madera, M., Maslen, J., McAnulla, C., McDowall, J., , Mistry, J., Mitchell, A., Nikolskaya, A. N., Orchard, S., Orengo, C., Petryszak, R., Selengut, J. D., Sigrist, C. J., Thomas, P. D., Valentin, F., Wilson, D., Wu, C. H. and Yeats, C. (2007). New developments in the InterPro database. Nucleic Acids Research 35, (Database issue). D224D228.
Muller, H.-M., Crampton, J. M., Della Torre, A., Sinden, R. E. and Frisanti, A. (1993). Members of a trypsin gene family in Anopheles gambiae are induced in the gut by bloodmeal. EMBO Journal 12, 28912900.
Naguleswaran, A., Alaeddine, F., Guionaud, C., Vonlaufen, N., Sonda, S., Jenoe, P., Mevissen, M. and Hemphill, A. (2005). Neospora caninum protein disulfide isomerase is involved in tachyzoite-host cell interaction. International Journal for Parasitology 35, 14591472.
Nijhout, M. M. and Carter, R. (1978). Gamete development in malarial parasites, bicarbonate-dependent stimulation by pH in vitro. Parasitology 76, 3953.
Nirmalan, N., Sims, P. F. G. and Hyde, J. E. (2004). Quantitative proteomics of the human malaria parasite Plasmodium falciparum and its application to studies of development and inhibition. Molecular Microbiology 52, 11871199.
Okamoto, N., Spurck, T. P., Goodman, C. D. and McFadden, G. I. (2008). The apicoplast and mitochondrion in gametocytogenesis of Plasmodium falciparum. Eukaryotic Cell 8, 128132.
Olszewski, K. L., Mather, M. W., Morrisey, J. M., Garcia, B. A., Vaidya, A. B., Rabinowitz, J. D. and Llinas, M. (2010). Branched tricarboxylic acid metabolism in Plasmodium falciparum. Nature 466, 774778.
Paton, M. G., Barker, G. C., Matsuoka, H., Ramesar, J., Janse, C. J., Waters, A. P. and Sinden, R. E. (1993). Structure and expression of a conserved and post-transcriptionally regulated gene encoding a surface protein of the sexual stages from malaria parasite Plasmodium berghei. Molecular and Biochemical Parasitology 59, 263275.
Peng, J., Elias, J. E., Thoreen, C. C., Licklider, L. J. and Gygi, S. P. (2003). Evaluation of multidimensional chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS). for large-scale protein analysis, the yeast proteome. Journal of Proteome Research 2, 4350.
Sam-Yellowe, T. Y., Del Rio, R. A., Fujioka, H., Aikawa, M., Yang, J.-C. and Yakubu, Z. (1998). Isolation of merozoite rhoptries, identification of novel rhoptry-associated proteins from Plasmodium yoelii, P. berghei and conserved interspecies reactivity of organelles and proteins with P. falciparum rhoptry-specific antibodies. Experimental Parasitology 89, 271284.
Sam-Yellowe, T. Y., Florens, L., Wang, T., Raine, J. D., Carucci, D., Sinden, R. E. and Yates, J. R. (2004). Proteome Analysis of Rhoptry-Enriched Fractions Isolated from Plasmodium Merozoites. Journal of Proteome Research 3, 9951001.
Scholz, S. M., Simon, N., Lavazec, C., Dude, M.-A., Templeton, T. J. and Pradel, G. (2007). PfCCp proteins of Plasmodium falciparum, Gametocyte-specific expression and role in complement-mediated inhibition of exflagellation. International Journal for Parasitology 38, 327340.
Shahabuddin, M. and Pimenta, P. F. P. (1998). Plasmodium gallinaceum preferentially invades vesicular ATPase-expressing cells in Aedes aegypti midgut. Proceedings of the National Academy of Sciences U.S.A. 95, 33853389.
Silvestrini, F., Bozdech, Z., Lanfrancotti, A., Di Guilio, E., Bultrini, E., Picci, L., deRisi, J. L., Pizzi, E. and Alano, P. (2005). Genome-wide identification of genes upregulated at the onset of gametocytogenesis in Plasmodium falciparum. Molecular and Biochemical Parasitology 143, 100110.
Sims, P. F. and Hyde, J. E. (2006). Proteomics of the human malaria parasite Plasmodium falciparum. Expert Reviews in Proteomics 3, 8795.
Sinden, R. E. (1978). Cell Biology. In Rodent Malaria. (ed. Killick-Kendrick, R. and Peters, W. A.), pp. 85186. Academic Press, New York.
Sinden, R. E. (1983). The cell biology of sexual development in plasmodium. Parasitology 86, 728.
Sinden, R. E., Canning, E. U. and Spain, B. (1976). Gametogenesis and fertilization in Plasmodium yoelii nigeriensis, a transmission electron microscope study. Proceedings of the Royal Society London B 193, 5576.
Sinden, R. E., Canning, E. U., Bray, R. S. and Smalley, M. E. (1978). Gametocyte and gamete development in Plasmodium falciparum. Proceedings of the Royal Society London B 201, 375399.
Sinden, R. E., Talman, A., Marques, S. R., Wass, M. N. and Sternberg, M. J. E. (2010). The flagellum in malarial parasites. Current Opinion in Microbiology 13, 491500.
Singh, S., Plassmeyer, M., Gaur, D. and Miller, L. H. (2007). Mononeme, a new secretory organelle in Plasmodium falciparum merozoites identified by localization of rhomboid-1 protease. Proceedings of the National Academy of Sciences U.S.A. 104, 2004320048.
Slavic, K., Delves, M. J., Prudencio, M., Talman, A. M., Straschil, U., Derbyshire, E. T., Xu, Z., Sinden, R. E., Mota, M. M., Morin, C., Tewari, R., Krishna, S. and Staines, H. M. (2011). Use of a selective inhibitor to define the chemotherapeutic potential of the plasmodial hexose transporter in different stages of the parasite's life cycle. Antimicrobial Agents and Chemotherapy 55, 28242830.
Southworth, P., Hyde, J. and Sims, P. (2011). A mass spectrometric strategy for absolute quantification of Plasmodium falciparum proteins of low abundance. Malaria Journal 10, 315.
Stanway, R. R. (2007). The ookinete surface proteome of Plasmodium berghei. PhD thesis. Imperial College London. 223p.
Straschil, U., Talman, A. M., Ferguson, D. J. P., Bunting, K. A., Xu, Z., Bailes, E., Sinden, R. E., Holder, A. A., Smith, E. F., Coates, J. C. and Tewari, R. (2010). The armadillo repeat protein PF16 is essential for flagellar structure and function in Plasmodium male gametes. PLoS One 5, e12901.
Talman, A. M. (2010). Molecular studies on Plasmodium sexual development. PhD thesis. Imperial College London, 176p.
Talman, A. M., Lacroix, C., Marques, S. R., Blagborough, A. M., Carzaniga, R., Ménard, R. and Sinden, R. E. (2011). PbGEST mediates malaria transmission to both mosquito and vertebrate host. Molecular Microbiology 82, 462474.
Tarun, A. S., Peng, X., Dumpit, R. F., Ogata, Y., Silva-Rivera, H., Camargo, N., Bergman, L. W. and Kappe, S. H. I. (2008). A combined transcriptome and proteome survey of malaria parasite liver stages. Proceedings of the National Academy of Sciences, USA 105, 305310.
Thompson, J. and Sinden, R. E. (1994). In situ detection of Pbs21 mRNA during sexual development of Plasmodium berghei. Molecular and Biochemical Parasitology 68, 189196.
Treeck, M., Sanders, J. L., Elias, J. E. and Boothroyd, J. C. (2011). The phosphoproteomes of Plasmodium falciparum and Toxoplasma gondii reveal unusual adaptations within and beyond the parasites' boundaries. Cell Host and Microbe 10, 410419.
Tufet-Bayona, M., Janse, C. J., Khan, S. M., Waters, A. P., Sinden, R. E. and Franke-Fayard, B. (2009). Localisation and timing of expression of putative Plasmodium berghei rhoptry proteins in merozoites and sporozoites. Molecular and Biochemical Parasitology 166, 2231.
van Schaijk, B. C. L., van Dijk, M. R., van de Vegte-Bolmer, M., van Gemert, G.-J., van Dooren, M. W., Eksi, S., Roeffen, W. F. G., Janse, C. J., Waters, A. P. and Sauerwein, R. W. (2006). Pfs47, paralog of the male fertility factor Pfs48/45, is a female specific surface protein in Plasmodium falciparum. Molecular and Biochemical Parasitology 149, 216222.
Vontas, J., Siden-Kiamos, I., Papagiannakis, G., Karras, M., Waters, A. P. and Louis, C. (2005). Gene expression in Plasmodium berghei ookinetes and early oocysts in a co-culture system with mosquito cells. Molecular and Biochemical Parasitology 139, 113.
Wang, Q., Brown, S., Roos, D. S., Nussenzweig, V. and Bhanot, P. (2004). Transcriptome of axenic liver stages of Plasmodium yoelii. Molecular and Biochemical Parasitology 137, 161168.
Wass, M. N., and Sternberg, M. J. E. (2008). ConFunc – functional annotation in the twilight zone. Bioinformatics 24, 798806.
Wass, M. N., Kelley, L. A. and Sternberg, M. J. E. (2010). 3DLigandSite, predicting ligand-binding sites using similar structures. Nucleic Acids Research 38, (Web Server issue), W469W473.
Watson, K., Edwards, R. J., Shaunak, S., Parmelee, D. C., Sarraf, C., Gooderham, N. J. and Davies, D. S. (1995). Extra-nuclear location of histones in activated human peripheral blood lymphocytes and cultured T-cells. Biochemical Pharmacology 50, 299309.
Wells, T. N. C., Alonso, P. L. and Gutteridge, W. E. (2009). New medicines to improve control and contribute to the eradication of malaria. Nature Reviews Drug Discovery 8, 879891.
Yeh, E. and DeRisi, J. L. (2011). Chemical rescue of malaria parasites lacking an apicoplast defines organelle function in blood-stage Plasmodium falciparum. PLoS Biology 9, e1001138.
Yu, C.-S., Chen, Y.-C., Lu, C.-H. and Hwang, J.-K. (2006). Prediction of protein subcellular localization. Proteins, Structure, Function, and Bioinformatics 64, 643651.


Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls

  • M. N. WASS (a1), R. STANWAY (a2) (a3), A. M. BLAGBOROUGH (a2), K. LAL (a2), J. H. PRIETO (a4), D. RAINE (a2), M. J. E. STERNBERG (a1), A. M. TALMAN (a2) (a5), F. TOMLEY (a6), J. YATES (a4) and R. E. SINDEN (a2)...


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