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Parasite identification, succession and infection pathways in perch fry (Perca fluviatilis): new insights through a combined morphological and genetic approach

Published online by Cambridge University Press:  02 January 2013

JASMINCA BEHRMANN-GODEL
JASMINCA BEHRMANN-GODEL*
Affiliation:
Limnological Institute, University of Konstanz, 78464 Konstanz, Germany
*
*Corresponding author: Tel: + + 49 7531 884536. Fax: + + 49 7531 883533. E-mail:Jasminca.Behrmann@uni-konstanz.de
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Summary

Identification of parasite species is particularly challenging in larval and juvenile hosts, and this hampers the understanding of parasite acquisition in early life. The work described here employs a new combination of methods to identify parasite species and study parasite succession in fry of perch (Perca fluviatilis) from Lake Constance, Germany. Classical morphological diagnostics are combined with sequence comparisons between parasite life-stages collected from various hosts within the same ecosystem. In perch fry at different stages of development, 13 different parasite species were found. Incomplete morphological identifications of cestodes of the order Proteocephalidea, and trematodes of the family Diplostomatidae were complemented with sequences of mitochondrial DNA (cytochrome oxidase 1) and/or nuclear (28 s rDNA) genes. Sequences were compared to published data and used to link the parasites in perch to stages from molluscs, arthropods and more easily identifiable developmental stages from other fishes collected in Lake Constance, which both aided parasite identification and clarified transmission pathways. There were distinct changes in parasite community composition and abundance associated with perch fry age and habitat shifts. Some parasites became more abundant in older fish, whereas the composition of parasite communities was more strongly affected by the ontogenetic shifts from the pelagic to the littoral zone.

Keywords

parasite communitytransmissionDiplostomatidaeProteocephalideabarcoding genes28s rDNACO1ITS1-5·8s-ITS2 rDNA
Type
Research Article
Information
Parasitology , Volume 140 , Issue 4 , April 2013 , pp. 509 - 520
Copyright
Copyright © Cambridge University Press 2013

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