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Molecular survey on cattle and sheep hydatidosis and first detection of Echinococcus canadensis (G6/G7) in sheep in Turkey

Published online by Cambridge University Press:  14 May 2020

Sarfraz Mehmood ,
Sami Simsek Open the ORCID record for Sami Simsek [Opens in a new window] ,
Figen Celik ,
Harun Kaya Kesik ,
Seyma Gunyakti Kilinc  and
Haroon Ahmed Open the ORCID record for Haroon Ahmed [Opens in a new window]
Sarfraz Mehmood
Affiliation:
Faculty of Veterinary Medicine, Department of Parasitology, University of Firat, 23119, Elazig, Turkey
Sami Simsek*
Affiliation:
Faculty of Veterinary Medicine, Department of Parasitology, University of Firat, 23119, Elazig, Turkey
Figen Celik
Affiliation:
Faculty of Veterinary Medicine, Department of Parasitology, University of Firat, 23119, Elazig, Turkey
Harun Kaya Kesik
Affiliation:
Faculty of Veterinary Medicine, Department of Parasitology, Bingol University, Bingol, Turkey
Seyma Gunyakti Kilinc
Affiliation:
Faculty of Veterinary Medicine, Department of Parasitology, University of Firat, 23119, Elazig, Turkey Faculty of Veterinary Medicine, Department of Parasitology, Bingol University, Bingol, Turkey
Haroon Ahmed
Affiliation:
Department of Biosciences, COMSATS University Islamabad, Park Road, Chakh Shahzad, Islamabad, Pakistan
*
Author for correspondence: Sami Simsek, E-mail: ssimsek@firat.edu.tr
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Abstract

Larval stage of genus Echinococcus is the causing agent for the zoonotic infection which is life threatening known as Echinococcosis. The purpose of this study was the identification, molecular analysis and characterization of Echinococcus spp. in sheep and cattle. The sampling was done from slaughterhouse of Elazig, Turkey. A total of 85 isolates (sheep, n = 19 and cattle, n = 66) have been collected after slaughtering. Following the gDNA isolation and PCR products of mt-CO1 gene (446 bp) of all the samples were sequenced. Out of 85 isolates, 84 were recognized as Echinococcus granulosus sensu stricto and one sheep isolate was found as Echinococcus canadensis (G6/G7 ) which is identified for the first time in Turkey. However, single nucleotide polymorphism has been observed not only in samples of different animals but also in samples collected from the same cattle. Six liver and three lung hydatid cysts have been detected in cattle. Although no nucleotide differences have been observed in the liver samples, there was single nucleotide polymorphism (C→T) in 40th nucleotide of two lung cysts. As a result of haplotype analysis, 16 haplotypes of E. granulosus s.s. were detected in 66 cattle isolates whereas 7 haplotypes of E. granulosus s.s. were identified in 19 sheep samples.

Keywords

CattleEchinococcus granulosushaplotypePCRsequencesheepTurkey
Type
Research Article
Information
Parasitology , Volume 147 , Issue 9 , August 2020 , pp. 1055 - 1062
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Copyright
Copyright © The Author(s) 2020. Published by Cambridge University Press

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