Skip to main content Accessibility help

A molecular phylogeny of the Dactylogyridae sensu Kritsky & Boeger (1989) (Monogenea) based on the D1-D3 domains of large subunit rDNA

  • A. šIMKOVÁ (a1), I. MATĚJUSOVÁ (a2) and C. O. CUNNINGHAM (a2)


Phylogenetic analyses based on the partial large subunit rDNA (LSU) sequences of polyonchoinean monogeneans belonging to the Dactylogyridea and Monocotylidea were generated to investigate relationships among various subfamilies of the Dactylogyridae sensu Kritsky & Boeger, 1989. Monophyly of the Dactylogyridae was supported by all analyses performed. Status of the Ancyrocephalidae sensu Bychowsky & Nagibina, 1978 and Ancyrocephalinae sensu Kritsky & Boeger, 1989 was revised based on the present data. All phylogenetic analyses indicated polyphyletic origins of the Ancyrocephalidae and Ancyrocephalinae. Freshwater species of Ancyrocephalinae (Actinocleidus, Ancyrocephalus, Cleidodiscus and Urocleidus) and Ancylodiscoidinae (Thaparocleidus) collected from the fish in European waters were positioned at the base of the Dactylogyridae. The Dactylogyrinae formed a monophyletic group, sister to a clade including the Pseudodactylogyrinae and the tropical and subtropical Ancyrocephalinae. Analyses including only data set on Dactylogyridea were focused on relationships between representatives of the Asian and European Dactylogyrus species. Dactylogyrus species formed a monophyletic group, and the parasite colonization appeared to follow the dispersal history of the Cyprinidae from Asia to Europe. Three lineages of Dactylogyrus species were recognized: the first including species specific to hosts of Asian origin, the second by Dactylogyrus species from Chinese fish hosts, and the third included Dactylogyrus species from European cyprinids and one species from a percid host. The position of D. cryptomeres from Gobio gobio seems to be unresolved.


Corresponding author

Institute of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, Brno 611 37, Czech Republic. Tel: +420549497363. Fax: +420541211214. E-mail:


Hide All


Beverley-Burton, M. ( 1984), Monogenea and Turbellaria. In Guide to the Parasites of Fishes of Canada. Part I. ( ed. Margolis, L. and Kabata, Z.), pp. 5204. Canadian Special Publication of Fisheries and Aquatic Sciences 74, Ottawa.
Bychowsky, B. E. ( 1937). Ontogenesis and phylogenetic relationships of Platyhelminthes. Izdatel'stvo Akademiya Nauk SSSR, Series Biology 4, 13531383 (in Russian).
Bychowsky, B. E. and Nagibina, L. F. ( 1978). Revision of Ancyrocephalinae Bychowsky, 1937. Parazitologicheskii Sbornik 28, 515.
Cunha, C., Mesquita, N., Dowling, T. E., Gilles, A. and Coelho, M. M. ( 2002). Phylogenetic relationships of Eurasian and American cyprinids using cytochrome b sequences. Journal of Fish Biology 61, 929944.
Desdevises, Y., Morand, S., Jousson, O. and Legendre, P. ( 2002). Coevolution between Lamellodiscus (Monogenea: Diplectanidae) and Sparidae (Teleostei): the study of a complex host-parasite system. Evolution 56, 24592471.
Durand, J.-D., Tsigenopoulos, C. S., Ünlü, E. and Berrebi, P. ( 2002). Phylogeny and biogeography of the family Cyprinidae in the Middle East inferred from cytochrome b DNA – evolutionary significance of this region. Molecular Phylogenetics and Evolution 22, 91100.
Felsenstein, J. ( 1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783791.
Hall, T. A. ( 1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 9598.
Hassouna, N., Michot, B. and Bachellerie, J.-P. ( 1984). The complete nucleotide sequence of mouse 28S rRNA gene. Implications for the process of size increase of the large subunit rRNA in higher eukaryotes. Nucleic Acids Research 12, 35633583.
Helfman, G. S., Collette, B. B. and Facey, D. E. ( 1997). The Diversity of Fishes. Blackwell Science, Madlen, MA.
Gibson, D. I., Timofeeva, T. A. and Gerasev, P. I. ( 1996). A catalogue of the nominal species of the monogenean genus Dactylogyrus Diesing, 1850 and their host genera. Systematic Parasitology 35, 348.
Gusev, A. V. ( 1985). Metazoan Parasites. Part I. Identification Key to Parasites of Freshwater Fish. Vol. 2, (Bauer, O. N.). Nauka, Leningrad (in Russian).
Jeanmougin, F., Thompson, J. D., Gouy, M., Higgins, D. G. and Gibson, T. J. ( 1998). Multiple sequence alignment with Clustal X. Trends in Biochemical Sciences 23, 403405.
Klassen, G. J. ( 1994 a). On the monophyly of Haliotrema species (Monogenea: Ancyrocephalidae) from boxfishes (Tetraodontiformes: Ostraciidae): relationships within the Bodiani group. Journal of Parasitology 75, 207211.
Klassen, G. J. ( 1994 b). Phylogeny of Haliotrema species (Monogenea, Ancyrocephalidae) from boxfishes (Tetraodontiformes, Ostraciidae) – are Haliotrema species from boxfishes monophyletic? Journal of Parasitology 80, 596610.
Kohn, A. and Cohen, S. ( 1998). South American Monogenea – list of species, hosts and geographical distribution. International Journal for Parasitology 28, 15171554.
Kritsky, D. C. and Boeger, W. A. ( 1989). The phylogenetic status of the Ancyrocephalidae Bychowsky, 1937 (Monogenea: Dactylogyroidea). Journal of Parasitology 75, 207211.
Kritsky, D. C. and Boeger, W. A. ( 2002). Neotropical Monogenoidea. 41: New and previously described species of Dactylogyridae (Platyhelminthes) from the gills of marine and freshwater perciform fishes (Teleostei) with proposal of a new genus and a hypothesis on phylogeny. Zoosystema 24, 740.
Lim, L. H. S. ( 1998). Diversity of monogeneans in Southeast Asia. International Journal for Parasitology 28, 14951515.
Littlewood, D. T. J., Rohde, K. and Clough, K. A. ( 1999 a). The interrelationships of all major groups of Platyhelminthes: phylogenetic evidence from morphology and molecules. Biological Journal of the Linnean Society 66, 75114.
Littlewood, D. T. J., Rohde, K., Bray, R. A. and Herniou, E. A. ( 1999 b). Phylogeny of the Platyhelminthes and the evolution of parasitism. Biological Journal of the Linnean Society 68, 257287.
Mollaret, I., Jamieson, B. G. M. and Justine, J.-L. ( 2000 a). Phylogeny of the Monopisthocotylea and Polyopisthocotylea (Platyhelminthes) inferred from 28S rDNA sequences. International Journal for Parasitology 30, 171185.
Mollaret, I., Lim, L. H. S. and Justine, J.-L. ( 2000 b). Phylogenetic position of the monogeneans Sundanonchus, Thaparocleidus, and Cichlidogyrus inferred from 28S rDNA sequences. International Journal for Parasitology 30, 659662.
Olson, P. D. and Littlewood, D. T. J. ( 2002). Phylogenetics of the Monogenea – evidence from a medley of molecules. International Journal for Parasitology 32, 233244.
Plaisance, L. and Kritsky, D. C. ( 2004). Dactylogyrids (Platyhelminthes: Monogenoidea) parasitizing butterflyfishes (Teleostei: Chaetodontidae) from the coral reefs of Palau, Moorea, Wallis, New Caledonia and Australia: Species of Euryhaliotrematoides gen. n. and Aliatrema gen. n. Journal of Parasitology 90, 328341.
Plaisance, L., Bouamer, S. and Morand, S. ( 2004). Description and a redescription of Haliotrema species (Monogenoidea: Polyonchoinea: Dactylogyridae) parasitizing butterflyfishes (Teleostei: Chaetodontidae) in the Indo-West Pacific Ocean. Parasitology Research 93, 598604.
Plaisance, L., Littlewood, D. T. J., Olson, P. D. and Morand, S. ( 2005). Molecular phylogeny of gill monogeneans (Platyhelminthes, Monogenea, Dactylogyridae) and colonization of Indo-West Pacific butterflyfish hosts (Perciformes, Chaetodontidae). Zoologica Scripta 34, 425436.
Posada, D. and Crandall, K. A. ( 1998). Modeltest: testing the model of DNA substitution. Bioinformatics 14, 817818.
Ronquist, F. and Huelsenbeck, J. P. ( 2003). MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 15721574.
šimková, A., Plaisance, L., Matějusová, I., Morand, S. and Verneau, O. ( 2003). Phylogenetic relationships of the Dactylogyridae Bychowsky, 1933 (Monogenea: Dactylogyridea): the need for the systematic revision of the Ancyrocephalinae Bychowsky, 1937. Systematic Parasitology 54, 111.
šimková, A., Morand, S., Jobet, E., Gelnar, M. and Verneau, O. ( 2004). Molecular phylogeny of congeneric monogenean parasites (Dactylogyrus): A case of intrahost speciation. Evolution 58, 10011018.
Swofford, D. L. ( 2002). PAUP* Phylogenetic Analysis using Parsimony and other Methods. Version 4.0b10. Sinauer Associates, Sunderland, Massachusetts.
Tsigenopoulos, C. S. and Berrebi, P. ( 2000). Molecular phylogeny of north Mediterranean freshwater barbs (genus Barbus: Cyprinidae) inferred from cytochrome b sequences: biogeographic and systematic implications. Molecular Phylogenetics and Evolution 14, 165179.
Winfield, I. J. and Nelson, J. S. ( 1991). Cyprinid Fishes. Systematics, Biology and Exploitation. Fish and Fisheries Series 3. Chapman and Hall, London.


A molecular phylogeny of the Dactylogyridae sensu Kritsky & Boeger (1989) (Monogenea) based on the D1-D3 domains of large subunit rDNA

  • A. šIMKOVÁ (a1), I. MATĚJUSOVÁ (a2) and C. O. CUNNINGHAM (a2)


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed