Hostname: page-component-8448b6f56d-mp689 Total loading time: 0 Render date: 2024-04-16T05:53:21.181Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular characterization and haplotypes of sheep and goat isolates of Cysticercus tenuicollis in Turkey

Published online by Cambridge University Press:  14 May 2019

Seyma Gunyakti Kilinc ,
Harun Kaya Kesik  and
Sami Simsek Open the ORCID record for Sami Simsek [Opens in a new window]
Seyma Gunyakti Kilinc
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119, Elazig, Turkey Department of Parasitology, Faculty of Veterinary Medicine, Bingol University, Bingol, Turkey
Harun Kaya Kesik
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, Bingol University, Bingol, Turkey
Sami Simsek*
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119, Elazig, Turkey
*
Author for correspondence: Sami Simsek, E-mail: ssimsek@firat.edu.tr
Get access Rights & Permissions [Opens in a new window]

Abstract

Taenia hydatigena, is a common parasite, lived mostly in dogs and wild carnivores in its mature stage, and the larvae, Cysticercus tenuicollis, is found on ruminants and pigs. The aim of the current study was to determine the genetic diversity in 20 isolates of the sheep and goats. After the isolation of total genomic DNA from C. tenuicollis isolates, genetic characterization of the mitochondrial NADH dehydrogenase subunit 1 gene region was amplified using specific JB11–JB12 primers in PCR and the PCR products were sequenced and haplotype and genetic diversity analyses were utilized. As a result, multiple nucleotide changes were determined in the sequence analyses of the isolates leading to detection of 16 and 15 different haplotypes in sheep and goat samples, respectively. These findings are important in terms of showing the diversity of nucleotide variation in C. tenuicollis in Turkey.

Keywords

Cysticercus tenuicollisgoathaplotypesheepTurkey
Type
Research Article
Information
Parasitology , Volume 146 , Issue 8 , July 2019 , pp. 1047 - 1054
Check for updates
Copyright
Copyright © Cambridge University Press 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adwan, K, Jayousi, A, Abuseir, S, Abbasi, I, Adwan, G and Jarrar, N (2018) Genetic diversity of Taenia hydatigena in the northern part of the West Bank, Palestine as determined by mitochondrial DNA sequences. Acta Parasitologica 63, 299–303.Google Scholar
Alachiotis, N, Vogiatzi, E, Pavlidis, P and Stamatakis, A (2013) Chromatogate: a tool for detecting base mis-calls in multiple sequence alignments by semi-automatic chromatogram inspection. Computational and Structural Biotechnology 6, 1–10.Google Scholar
Anon (2018) Turkish Statistical Institute. Provincial Indicators Database.Google Scholar
Aydın, A (2003) Hakkari belediye mezbahasında kesilen hayvanlarda paraziter fauna tespit çalışmaları. Doktora Tezi. YY U. Sağlık Bilimleri Enstitüsü, Parazitoloji Programı. Van.Google Scholar
Bamorovat, M, Radfar, MH, Derakhshanfar, A, Molazadeh, M and Zarandi, MB (2014) A comparative evaluation of hematological, biochemical and pathological changes among infected sheep with Cysticercus tenuicollis and non-infected control group. Journal of Parasitic Disease 38, 399–403.Google Scholar
Blazek, K, Schramlova, J and Hulinska, D (1985) Pathology of the migration phase of Taenia hydatigena (Pallas, 1766) larvae. Folia Parasitologia 32, 127–137.Google Scholar
Boufana, B, Scala, A, Lahmar, S, Pointing, S, Craig, PS, Dessì, G, Zidda, A, Pipia, AP and Varcasia, A (2015) A preliminary investigation into the genetic variation and population structure of Taenia hydatigena from Sardinia, Italy. Veterinary Parasitology 214, 67–74.Google Scholar
Bowles, J, Blair, D and McManus, DP (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology 54, 165–174.Google Scholar
Deger, S and Bicek, K (2005) Tatvan belediye mezbahasında kesilen koyun, keçi ve sığırlarda larval cestodiosis. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi 16(1), 45–47.Google Scholar
Deger, S, Bicek, K, Gul, A and Eraslan, E (2001) Van yöresinde koyun keçi ve sığırlarda, Cysticercus tenuicollis'in yaygınlığı. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi 7, 95–97.Google Scholar
Deplazes, P (2006) Helminthosen von hund und katze. In Veterinarmedizinische Parasitologie. Schnieder, Hrsg. T., Buchverlag, Parey, 444–518.Google Scholar
Edwards, G and Herbert, I (1980) The course of Taenia hydatigena infections in growing pigs and lambs: clinical signs and post-mortem examination. British Veterinary Journal 136, 256–264.Google Scholar
Kara, M, Gicik, Y, Sari, B, Bulut, H and Arslan, MA (2009) Slaughterhouse study on prevalence of some helminths of cattle and sheep in Malatya province, Turkey. Journal of Animal and Veterinary Advances 8, 2200–2205.Google Scholar
Kaufmann, J (2013) Parasitic Infections of Domestic Animals: A Diagnostic Manual. Basel-Boston-Berlin: Birkhauser; 1996.Google Scholar
Murrell, KD, Dorny, P, Flisser, A, Geerts, S, Kyvsgaard, NC and McManus, DP (2005) WHO/FAO/OIE guidelines for the surveillance, prevention and control of taeniasis/cysticercosis. Oie (world organisation for animal health). WHO (World Health Organization) and FAO (Food and Agriculture Organization).Google Scholar
Oncel, T (2000) The prevalence of helminth species in sheep in the southern region of Marmara. Türkiye Parazitoloji Dergisi 24, 414–419.Google Scholar
Payan-Carreira, R, Silva, F, Rodrigues, M and dos Anjos Pires, M (2008) Cysticercus tenuicollis vesicle in fetal structures: report of a case. Reproduction in Domestic Animals 43, 764–766.Google Scholar
Sarimehmetoglu, H, Gonenc, B, Piskin, C and Ayaz, E (1993) Koyun, keçi, sığır ve mandalarda Cysticercus tenuicollis’ in yayılışı. Ankara Üniversitesi Veteteriner Fakültesi Dergisi 40, 488–496.Google Scholar
Scala, A, Garippa, G, Varcasia, A, Tranquillo, V and Genchi, C (2006) Cystic echinococcosis in slaughtered sheep in Sardinia (Italy). Veterinary Parasitology 135, 33–38.Google Scholar
Scala, A, Pipia, AP, Dore, F, Sanna, G, Tamponi, C, Marrosu, R, Bandino, E, Carmona, C, Boufana, B and Varcasia, A (2015) Epidemiological updates and economic losses due to Taenia hydatigena in sheep from Sardinia, Italy. Parasitology Research 114, 3137–3143.Google Scholar
Senlik, B (2008) Influence of host breed, sex and age on the prevalence and intensity of Cysticercus tenuicollis in sheep. Journal of Animal Veterinary Advances 7, 548–551.Google Scholar
Singh, BB, Sharma, R, Gill, JP and Sharma, JK (2015) Prevalence and morphological characterization of Cysticercus tenuicollis (Taenia hydatigena cysts) in sheep and goat from north India. Journal of Parasitic Disease 39, 80–84.Google Scholar
Soulsby, E. J. L. (1982). Helminths, Arthropods and Protozoa of Domesticated Animals. London: Bailliere Tindall.Google Scholar
Taylor, M, Coop, R and Wall, R (2007) Veterinary Parasitology. Oxford, UK. Google Scholar: Blackwell Publishing.Google Scholar
Utuk, AE and Piskin, FC (2012) Molecular detection and characterization of goat isolate of Taenia hydatigena in Turkey. Scientific World Journal 2012, 962732.Google Scholar
Zeybek, H (1980) Samsun yöresi koyun ve kuzularında paraziter fauna saptama çalışmaları. Ankara Üniversitesi Veteriner Fakültesi Dergisi 27, 215–236.Google Scholar