Hostname: page-component-8448b6f56d-sxzjt Total loading time: 0 Render date: 2024-04-23T21:19:32.964Z Has data issue: false hasContentIssue false
  • English
  • Français

Lipid content and fatty acid composition of the monogenean Neobenedenia girellae and comparison between the parasite and host fish species

Published online by Cambridge University Press:  04 July 2008

S. SATO ,
T. HIRAYAMA  and
N. HIRAZAWA
S. SATO*
Affiliation:
Central Research Laboratories, Nippon Suisan Kaisha, Ltd, 559-6 Kitanomachi, Hachioji, Tokyo 192-0906, Japan
T. HIRAYAMA
Affiliation:
Marine Biological Technology Center, Nippon Suisan Kaisha, Ltd, Saeki, Oita 876-1204, Japan
N. HIRAZAWA
Affiliation:
Central Research Laboratories, Nippon Suisan Kaisha, Ltd, 559-6 Kitanomachi, Hachioji, Tokyo 192-0906, Japan
*
*Corresponding author: Central Research Laboratories, Nippon Suisan Kaisha, Ltd, 559-6 Kitanomachi, Hachioji, Tokyo 192-0906, Japan. Tel: +81 426 56 5192. Fax: +81 426 56 5188. E-mail: s-satou@nissui.co.jp
Get access Rights & Permissions [Opens in a new window]

Summary

Neobenedenia girellae, a capsalid monogenean, is a destructive fish parasite. We studied the lipid content and fatty acid composition of N. girellae and the skin and cutaneous mucus of a host fish, the amberjack Seriola dumerili (Carangidae). The lipid content of adult N. girellae was less than one-fourth that of both the skin and cutaneous mucus of its host. Adult N. girellae, S. dumerili skin and mucus had a relatively high weight-percentage of C16:0, C18:1(n-9), C18:0 and C22:6(n-3) fatty acids. When S. dumerili were fed a diet supplemented with [13C] fatty acids, [13C] fatty acids were detected in S. dumerili skin and adult N. girellae on S. dumerili, but no [13C] fatty acids were detected in the S. dumerili cutaneous mucus. In addition, the epidermis of S. dumerili, attached with N. girellae, was markedly thin. These results suggest that N. girellae feeds primarily on host epithelial cells. We then infected 2 host fishes, S. dumerili and the spotted halibut Verasper variegatus (Pleuronectidae; a host less susceptible to N. girellae infection), and compared the fatty acid composition of N. girellae with that of the skin and cutaneous mucus of the hosts. The fatty acid profiles from all samples were qualitatively and quantitatively similar. Thus, the fatty acid composition of the host may not contribute to the difference in susceptibility between S. dumerili and V. variegatus. These results may serve to develop new strategies for the control of N. girellae infections.

Keywords

MonogeneaNeobenedenia girellaelipid contentfatty acid compositionSeriola dumeriliVerasper variegatus
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 8 , July 2008 , pp. 967 - 975
Copyright
Copyright © 2008 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bondad-Reantaso, M. G., Ogawa, K., Fukudome, M. and Wakabayashi, H. (1995). Reproduction and growth of Neobenedenia girellae (Monogenea: Capsalidae), a skin parasite of cultured marine fishes of Japan. Fish Pathology 30, 227–231.CrossRefGoogle Scholar
Dixon, H. and Williamson, J. (1970). The lipid composition of blood and culture forms of Trypanosoma lewisi and Trypanosoma rhodesiense compared with that of their environment. Comparative Biochemistry and Physiology 33, 111–128.CrossRefGoogle ScholarPubMed
Furlong, S. T. (1991). Unique roles for lipids in Schistosoma mansoni. Parasitology Today 7, 59–62.Google Scholar
Haas, W. and Van de Roemer, A. (1998). Invasion of the vertebrate skin by cercariae of Trichobilharzia ocellata: penetration processes and stimulating host signals. Parasitology Research 84, 787–795.CrossRefGoogle ScholarPubMed
Hatanaka, A., Umeda, N., Yamashita, S. and Hirazawa, N. (2005). A small ciliary surface glycoprotein of the monogenean parasite Neobenedenia girellae acts as an agglutination/immobilization antigen and induces an immune response in the Japanese flounder Paralichthys olivaceus. Parasitology 131, 591–600.CrossRefGoogle ScholarPubMed
Hirazawa, N., Hara, T., Mitsuboshi, T, Okazaki, J. and Hata, K. (1999) Iodophor disinfection of eggs of spotted halibut Verasper variegatus and red sea bream Pagrus major. Fisheries Science 65, 333–338.CrossRefGoogle Scholar
Hirazawa, N., Mitsuboshi, T., Hirata, T. and Shirasu, K. (2004). Susceptibility of spotted halibut Verasper variegatus (Pleuronectidae) to infection by the monogenean Neobenedenia girellae (Capsalidae) and oral therapy trials using praziquantel. Aquaculture 238, 83–95.CrossRefGoogle Scholar
Kearn, G. C., Ogawa, K. and Maeno, Y. (1992). Egg production, the oncomiracidium and larval development of Benedenia seriolae, a skin parasite of the yellowtail, Seriola quinqueradiata in Japan. Publications of the Seto Marine Biological Laboratory 35, 351–362.CrossRefGoogle Scholar
Kimoto, K. and Sato, K. (2002). Cultivation experiments of spotted halibut. Oita Prefectural Fish Research Center Report, 139–151. (In Japanese.)Google Scholar
Kumai, H. (2000). Aquaculture of Marine Fishes. Sobunsha, Tokyo. (In Japanese.)Google Scholar
Leong, T. S. (1997). Control of parasites in cultured marine finfishes in Southeast Asia-an overview. International Journal for Parasitology 27, 1177–1184.Google Scholar
Mi-ichi, F., Kita, K. and Mitamura, T. (2006). Intraerythrocytic Plasmodium falciparum utilize a broad range of serum-derived fatty acid with limited modification for their growth. Parasitology 133, 399–410.Google Scholar
Mitamura, T., Hanada, K., Ko-Mitamura, E. P., Nishijima, M. and Horii, T. (2000). Serum factors governing intraerythrocytic development and cell cycle progression of Plasmodium falciparum. Parasitology International 49, 219–229.CrossRefGoogle ScholarPubMed
Ogawa, K., Bondad-Reantaso, M. G., Fukudome, M. and Wakabayashi, H. (1995). Neobenedenia girellae (Hargis, 1955) Yamaguti, 1963 (Monogenea: Capsalidae) from cultured marine fishes of Japan. Journal of Parasitology 81, 223–227.Google Scholar
Ogawa, K. and Yokoyama, H. (1998). Parasitic diseases of cultured marine fish in Japan. Fish Pathology 33, 303–309.CrossRefGoogle Scholar
Ohno, Y., Kawano, F. and Hirazawa, N. (2008). Japanese flounder (Paralichthys olivaceus) to Neobenedenia girellae (Monogenea) infection and their acquired protection. Aquaculture 274, 30–35.Google Scholar
Paperna, I. (1991). Diseases caused by parasites in the aquaculture of warm water fish. Annual Review of Fish Diseases 1, 155–194.CrossRefGoogle Scholar
Shephard, K. L. (1994). Functions for fish mucus. Reviews in Fish Biology and Fisheries 4, 401–429.Google Scholar
Sherman, I. W. (1998). Malaria: Parasite Biology, Pathogenesis, and Protection. ASM Press, Washington, DC, USA.Google Scholar
Soudant, P. and Chu, F. E. (2001). Lipid class and fatty acid composition of the protozoan parasite of oysters, Perkinsus marinus cultivated in two different media. The Journal of Eukaryotic Microbiology 48, 309–319.Google Scholar
Uenokawa, S. and Shirahata, S. (2000). Animal Cell Technology Handbook. Asakura, Tokyo.Google Scholar
Willoughby, L. G. (1971). Observations on fungal parasites of Lake District salmonids. The Salmon and Trout Magazine 192, 152–158.Google Scholar
Willoughby, L. G. (1972). U.D.N. of Lake District trout and char: outward signs of infection and defence barriers examined further. The Salmon and Trout Magazine 195, 149–158.Google Scholar
Wilson, R. A. and Denison, J. (1970). Short chain fatty acids as stimulants of turning activity by the miracidium of Fasciola hepatica. Comparative Biochemistry and Physiology 32, 511–517.Google Scholar
Woo, P. T. K., Bruno, D. W. and Lim, L. H. S. (2002). Diseases and Disorders of Finfish in Cage Culture. CABI Publishing, London, UK.CrossRefGoogle Scholar