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In vitro cytoadherence of Plasmodium falciparum-infected erythrocytes to melanoma cells: factors affecting adhesion

  • I. W. Sherman (a1) and Esther Valdez (a1)

Summary

A reproducible and standardized assay for measuring the cytoadherence of knobby Plasmodium falciparum-infected red cells to amelanotic melanoma cells was developed. Adhesion was dependent on temperature, haematocrit, and parasitaemia. Addition of EDTA to the binding medium reduced adhesion. Removal of protease-sensitive molecules on the surface of the infected cell abolished cytoadherence, whereas removal of carbohydrate residues by treatment of cells with neuraminidase or galactosidase promoted adhesion. Calcium, magnesium, fibrinogen or fibronectin in the medium had no effect on adhesion nor was there any enhancement of adhesion by pre-loading infected cells with calcium. Serum was essential for good adhesion. Adhesion was species specific for target cells; human endothelial or amelanotic melanoma cells were suitable target cells whereas bovine cells were not. The amelanotic melanoma cell could be formalin-fixed and still retain its adhesion properties. The binding properties of formalin-fixed amelanotic melanoma cells were not identical to those of endothelial or unfixed target cells.

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Aikawa, M., Rabbege, J. R., Udeinya, I. & Miller, L. H. (1983). Electron microscopy of knobs in Plasmodium falciparum-infected erythrocytes. Journal of Parasitology 69, 435–7.
Aley, A. B., Sherwood, J. A. & Howard, R. J. (1984). Knob-positive and knob-negative Plasmodium falciparum differ in expression of a strain-specific malarial antigen on the surface of infected erythrocytes. Journal of Experimental Medicine 160, 1585–90.
Aley, S. B., Barnwell, J. W., Daniel, W. A. & Howard, R. J. (1984). Identification of parasite proteins in a membrane preparation enriched for the surface membrane of erythrocytes infected with Plasmodium knowlesi. Molecular and Biochemical Parasitology 12, 6984.
Barnwell, J. W., Howard, R. J. & Miller, L. H. (1983). Influence of the spleen on the expression of surface antigens on parasitized erythrocytes. Ciba Foundation Symposium 94, 117–36.
David, P. H., Hommel, M., Miller, L. H., Udeinya, I. J. & Oligino, L. D. (1983). Parasite sequestration in Plasmodium falciparum malaria: spleen and antibody modulation of cytoadherence of infected erythrocytes. Proceedings of the National Academy of Sciences, USA 80, 5075–9.
Fukuda, M. N., Fukuda, M. & Hakomori, S.-I. (1979). Cell surface modification by endo-β-galactosidase. Change of blood group activities and release of oligosaccharides from glycoproteins and cyclosphingolipids of human erythrocytes. Journal of Biological Chemistry 254(12), 5488–65.
Gruenberg, J., Allred, D. R. & Sherman, I. W. (1983). Scanning electron microscope-analysis of the protrusions (knobs) present on the surface of Plasmodium falciparum-infected erythrocytes. Journal of Cell Biology 97, 795802.
Harlan, J. M. (1985). Leukocyte-endothelial interactions. Blood 65(3), 513–25.
Hoover, R. L. & Karnovsky, M. J. (1984). Adhesive interactions between polymorphonuclear leukocytes and endothelium. In Biology of Endothelial Cells (ed. Jaffe, E. A.), pp. 277–85. Boston: Martinus Nijhoff.
Howard, R. J., Kaushal, D. & Carter, R. (1982). Plasmodium gallinaceum: radioiodination of parasite antigens with l,3,4,6-tetrachloro)-3α,6α-diphenylglycoluril (Iodogen). Studies with zygotes of P. gallinaceum. Journal of Protozoology 29, 114–17.
Jaffe, E. A., Nachman, R. L., Becker, C. G. & Minick, C. R. (1973). Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. Journal of Clinical Investigation 52, 2745–56.
Lambros, C. & Vanderberg, J. P. (1980). Synchronization of Plasmodium falciparum erythrocytic stages in culture. Journal of Parasitology 65, 418–20.
Langreth, S. G., Jensen, J. B., Reese, R. T. & Trager, W. (1978). Fine structure of human malaria in vitro. Journal of Protozoology 25, 443–52.
Langreth, S. G., Motyl, M. R. & Trager, W. (1979). Plasmodium falciparum: loss of knobs on the infected erythrocyte surface after long term cultivation. Experimental Parasitology 48, 213–19.
Leech, J. H., Barnwell, J. W., Miller, L. H. & Howard, R. J. (1984). Identification of a strain-specific malarial antigen exposed on the surface of Plasmodium falciparum-infected erythrocytes. Journal of Experimental Medicine 159, 1567–75.
Macpherson, G. G., Warrell, M. J., White, N. J., Looareesuwan, S. & Warrell, D. A. (1985). Human cerebral malaria: a quantitative ultrastructural analysis of parasitized erythrocyte sequestration. American Journal of Pathology 119, 385401.
Marsh, K., Marsh, V. M., Brown, J., Whittle, H. C. & Greenwood, B. M. (1988). Plasmodium falciparum: the behavior of clinical isolates in an in vitro model of infected red blood cell sequestration. Experimental Parasitology 65, 202–8.
Mohandas, N. & Evans, E. (1984). Adherence of sickle erythrocytes to vascular endothelial cells: requirement for both cell membrane changes and plasma factors. Blood 64, 282–7.
Ockenhouse, C. F. & Chulay, J. D. (1988). Plasmodium falciparum sequestration: OKM5 antigen (DC36) mediates cytoadherence of parasitized erythrocytes to a myelomonocytic cell line. Journal of Infectious Diseases 157(3), 584–8.
Pasvol, G., Wilson, J. M., Smalley, M. E. & Brown, J. (1978). Separation of viable schizont-infected red cells of Plasmodium falciparum from human blood. Annals of Tropical Medicine and Parasitology 72, 87–8.
Pongponratn, E., Riganti, M., Harinasuta, T. & Bunnag, D. (1985). Electron microscopy of the human brain in cerebral malaria. Southeast Asian Journal of Tropical Medicine and Public Health 16(2), 219–27.
Raventos-Suarez, C., Kaul, D. K., Maculuso, F. & Nagel, R. L. (1985). Membrane knobs are required for the microcirculatory obstruction induced by Plasmodium falciparum-infected erythrocytes. Proceedings of the National Academy of Sciences, USA 82, 3829–33.
Roberts, D. D., Sherwood, J. A., Spitalnik, S. L., Panton, L. J., Howard, R. J., Dixit, V. M., Frazier, W. A., Miller, L. H. & Ginsburg, V. (1985). Thrombospondin binds falciparum malaria parasitized erythrocytes and may mediate cytoadherence. Nature, London 318, 64–6.
Schlegel, R. A., Prendergast, T. W. & Williamson, P. (1985). Membrane phospholipid asymmetry as a factor in erythrocyte-endothelial cell interactions. Journal of Cellular Physiology 123, 215–18.
Schmidt, J. A., Udeinya, I. J., Leech, J. H., Hay, R. J., Aikawa, M., Barnwell, J., Green, I. & Miller, L. H. (1982). Plasmodium falciparum malaria: an amelanotic melanoma cell line bears receptors for the knob ligand on infected erythrocytes. Journal of Clinical Investigation 70, 379–86.
Shepro, D., Batbouta, J. C., Robblee, L. S., Carson, M. P. & Belamarich, F. A. (1975). Serotonin transport by cultured bovine aortic endothelium. Circulation Research 36, 799806.
Sherman, I. (1985). Membrane structure and function of malaria parasites and the infected erythrocyte. Parasitology 91, 609–45.
Sherman, I. W. & Greenan, J. R. T. (1986). Plasmodium falciparum: regional differences in lectin and cationized ferritin binding to the surface of the malaria-infected human erythrocyte. Parasitology 93, 1732.
Sherwood, J. A., Roberts, D. D., Marsh, K., Harvery, E. B., Spitalnik, S. L., Miller, L. H. & Howard, R. J.(1987). Thrombospondin binding by parasitized erythrocyte isolates in falciparum malaria. American Journal of Tropical Medicine and Hygiene 36(2), 228–33.
Singh, B., Monsigny, M. & Hommel, M. (1987). Amino-sugars inhibit the in vitro cytoadherence of Plasmodium falciparum-infected erythrocytes to melanoma cells. Molecular and Biochemical Parasitology 23, 4753.
Trager, W. & Jensen, J. B. (1976). Human malaria parasites in continuous culture. Science 193, 673–5.
Trager, W., Tershakovec, M., Lyandvert, L., Stanley, H., Lanners, N. & Gubert, E. (1981). Clones of the malaria parasite Plasmodium falciparum obtained by microscopic selection – their characterization with regard to knobs, chloroquine sensitivity, and formation of gametocytes. Proceedings of the National Academy of Sciences, USA 78, 6527–30.
Udeinya, I. J., Graves, P. M., Carter, R. & Aikawa, M. (1983). Plasmodium falciparum: effect of time in continuous culture on binding to human endothelial cells and amelanotic melonoma cells. Experimental Parasitology 56, 207–14.
Udeinya, I. J., Leech, J., Aikawa, M. & Miller, L. H. (1985). An in vitro assay for sequestration: binding of Plasmodium falciparum-infected erythrocytes to formalin-fixed endothelial cells and amelanotic melanoma cells. Journal of Protozoology 32(1), 8890.
Udeinya, I. J., Schmidt, J. A., Aikawa, M., Miller, L. H. & Green, I. (1981). Falciparum malaria-infected erythrocytes specifically bind to cultured human endothelial cells. Science 213, 555–7.
Warrell, D. A. (1987). Pathophysiology of severe falciparum malaria in man. Parasitology 94, S53S76.
Wautier, J. L., Pintigny, D., Wautier, M. P., Paton, R. C., Galacteros, F., Passa, P. & Caen, J. P. (1983). Fibrinogen, a modulator of erythrocyte adhesion to vascular endothelium. Journal of Laboratory and Clinical Medicine 101(6), 911–20.
Wickramasinghe, S. N., Phillips, R. E., Looareesuwan, S., Warrell, D. A. & Hughes, M. (1987). The bone marrow in human cerebral malaria: parasite sequestration within sinusoids. British Journal of Haematology 66, 295306.

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In vitro cytoadherence of Plasmodium falciparum-infected erythrocytes to melanoma cells: factors affecting adhesion

  • I. W. Sherman (a1) and Esther Valdez (a1)

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