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In vitro and ex vivo activity of Melaleuca alternifolia against protoscoleces of Echinococcus ortleppi

Published online by Cambridge University Press:  20 October 2016

DANIELI URACH MONTEIRO ,
MARIA ISABEL AZEVEDO ,
CARLA WEIBLEN ,
SÔNIA DE AVILA BOTTON ,
NADINE LYSYK FUNK ,
CRISTIANE DE BONA DA SILVA ,
RÉGIS ADRIEL ZANETTE ,
THIAGO GUILHERME SCHWANZ  and
MÁRIO LUIZ DE LA RUE
DANIELI URACH MONTEIRO
Affiliation:
Postgraduation Program in Pharmaceutical Sciences, Universidade Federal de Santa Maria, UFSM, Santa Maria, Brazil
MARIA ISABEL AZEVEDO
Affiliation:
Department of Industrial Pharmacy, Postgraduation Program in Veterinary Medicine, Universidade Federal de Santa Maria, UFSM, Santa Maria, Brazil
CARLA WEIBLEN
Affiliation:
Department of Industrial Pharmacy, Postgraduation Program in Veterinary Medicine, Universidade Federal de Santa Maria, UFSM, Santa Maria, Brazil
SÔNIA DE AVILA BOTTON
Affiliation:
Department of Industrial Pharmacy, Postgraduation Program in Veterinary Medicine, Universidade Federal de Santa Maria, UFSM, Santa Maria, Brazil
NADINE LYSYK FUNK
Affiliation:
Postgraduation Program in Pharmaceutical Sciences, Universidade Federal de Santa Maria, UFSM, Santa Maria, Brazil
CRISTIANE DE BONA DA SILVA
Affiliation:
Postgraduation Program in Pharmaceutical Sciences, Universidade Federal de Santa Maria, UFSM, Santa Maria, Brazil
RÉGIS ADRIEL ZANETTE
Affiliation:
Postgraduation Program in Biological Sciences: Pharmacology and Therapeutics, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
THIAGO GUILHERME SCHWANZ
Affiliation:
Postgraduation Program in Pharmaceutical Sciences, Universidade Federal de Santa Maria, UFSM, Santa Maria, Brazil
MÁRIO LUIZ DE LA RUE*
Affiliation:
Postgraduation Program in Pharmaceutical Sciences, Universidade Federal de Santa Maria, UFSM, Santa Maria, Brazil
*
*Corresponding author. Departamento de Microbiologia e Parasitologia, Campus Universitário, Universidade Federal de Santa Maria, Avenida Roraima, 1000, Prédio 20 – Sala 4226, CEP 97105-970, Santa Maria, RS, Brazil. E-mail: mldelarue@hotmail.com
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Summary

Cystic echinococcosis is a zoonotic disease of difficult diagnosis and treatment. The use of protoscolicidal agents in procedures is of utmost importance for treatment success. This study was aimed at analysing the in vitro and ex vivo activity of Melaleuca alternifolia oil (tea tree oil – TTO), its nanoemulsion formulation (NE-TTO) and its major component (terpinen-4-ol) against Echinococcus ortleppi protoscoleces obtained from cattle. Concentrations of 2·5, 5 and 10 mg mL−1 of TTO, 10 mg mL−1 of NE-TTO and 1, 1·5 and 2 mg mL−1 of terpinen-4-ol were evaluated in vitro against protoscoleces at 5, 10, 15 and 30 min. TTO was also injected directly into hydatid cysts (ex vivo analysis, n = 20) and the viability of protoscoleces was evaluated at 5, 15 and 30 min. The results indicated protoscolicidal effect at all tested formulations and concentrations. Terpinen-4-ol (2 mg mL−1) activity was superior when compared with the highest concentration of TTO. NE-TTO reached a gradual protoscolicidal effect. TTO at 20 mg mL−1 showed 90% protoscolicidal action in hydatid cysts at 5 min. The results showed that TTO affects the viability of E. ortleppi protoscoleces, suggesting a new protoscolicidal option to the treatment of cystic equinococcosis.

Keywords

Echinococcus ortleppitea tree oilprotoscolicidalnanoemulsions
Type
Research Article
Information
Parasitology , Volume 144 , Issue 2 , February 2017 , pp. 214 - 219
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Copyright
Copyright © Cambridge University Press 2016 

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