Skip to main content Accessibility help
×
Home

Identification and characterization of a polysaccharide-containing antigen from Schistosoma mansoni eggs which cross-reacts with the surface of schistosomula

  • D. W. Dunne (a1) and Q. D. Bickle (a1)

Summary

Antisera were produced by immunizing rabbits with either a trichloroacetic acid-soluble fraction, or a high molecular weight (Mr) fraction of Schistosoma mansoni SEA (a salinesoluble fraction of homogenized egg). Both of these sera reacted monospecifically in immunoelectrophoresis against unfractionated SEA, recognizing a cathodally migrating antigen. This antigen had been identified previously as being responded to by S. mansoni-infected mouse sera, and has been designated K3 (Kappa 3). The rabbit antisera were used to partially characterize antigen K3 as having Mr in the range > 750 – 70 K, and being resistant to boiling, resistant to the action of proteases, but sensitive to periodate. It partially binds to Concanavalin A. In addition to SEA, the antigen was present in homogenized cercariae and schistosomula, but not adult worms, and it was also present in detergent extracts of intact cercariae and schistosomula. Using an antibody-dependent cell adherence assay, anti-K3 serum was found to react with the surface of live cercariae and with the surface of schistosomula recovered from the skin of mice infected up to 48 h previously. Anti-K3 serum also reacted with the surface of S. bovis, S. haematobium and to a lesser extent S. japonicum schistosomula.

Copyright

References

Hide All
Bickle, Q. D., Andrews, B. J. & Taylor, M. G. (1986). Schistosoma mansoni: characterisation of two-protective monoclonal antibodies. Parasite Immunology 8, 95107.
Bickle, Q. D. & Ford, M. J. (1982). Studies on the surface antigenicity and susceptibility to antibody-dependent killing of developing schistosomula using sera from chronically infected mice and mice vaccinated with irradiated cercariae. Journal of Immunology 128, 2101–6.
Bickle, Q. D., Ford, M. J. & Andrews, B. J. (1983). Studies on the development of antischistosomular surface antibodies by the mice exposed to irradiated cercariae. adults and/or eggs of S. mansoni. Parasite Immunology 5, 499511.
Boctor, F. N., Cheever, A. W. & Higashi, G. I. (1982). Schistosoma mansoni fractionation of polysaccharide egg antigens by lectin affinity chromatography. Immunology 45, 237–45.
Boctor, F. N., Nash, T. E. & Cheever, A. W. (1979). Isolation of a polysaccharide antigen from Schistosoma mansoni. Journal of Immunology 122, 3943.
Butterworth, A. E. (1984). Cell-mediated damage to helminths. Advances in Parasitology 23, 143235.
Dean, D. A. (1977). Decreased binding of cytotoxic antibody by developing Schistosoma mansoni. Evidence for a surface change independent of host adsorption and membrane turnover. Journal of Parasitology 63, 418–26.
Dissous, C. & Capron, A. (1981). Isolation of surface antigens from Schistosoma mansoni schistosomula. In Protides of the Biological Fluids (ed. Peeters, H.). pp. 179–82. Oxford and New York: Pergamon Press.
Dissous, C., Grzych, J. M. & Capron, A. (1982). Schistosoma mansoni surface antigen defined by a rat monoclonal IgG2a. Journal of Immunology 129, 2232–4.
Doenhoff, M. J., Pearson, S., Dunne, D. W., Bickle, Q., Lucas, S., Bain, J. & Hassounah, O. (1981). Immunological control of hepatotoxicity and parasite egg excretion in Schistosoma mansoni infections: stage and species specificity of the reactivity of immune serum in T-cell deprived mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 75. 4153.
Dresden, M. H., Sung, C. K. & Deelder, A. M. (1983). A monoclonal antibody from infected mice to a Schistosoma mansoni egg proteinase. Journal of Immunology 130, 13.
Dunne, D. W., Bain, J., Lillywhite, J. & Doenhoff, M. J. (1984). The stage-, strain- and species-specificity of a Schistosoma mansoni egg antigen fraction (CEF6) with serodiagnostic potential. Transactions of the Royal Society of Tropical Medicine and Hygiene 78, 460–70.
Dunne, D. W., Bickle, Q. D., Butterworth, A. E. & Richardson, B. A. (1987). The blocking of human antibody-dependent, eosinophil-mediated killing of Schistosoma mansoni schistosomula by monoclonal antibodies which cross-react with a polysaccharide-containing egg antigen. Parasitology 94, 269–80.
Dunne, D. W., Lucas, S., Bickle, Q., Pearson, S., Madgwick, L., Bain, J. & Doenhoff, M. J. (1981). Identification and partial purification of an antigen (ω1) from Schistosoma mansoni eggs which is putatively hepatotoxic in T-cell deprived mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 5471.
Grzych, J. M., Capron, M., Bazin, H. & Capron, A. (1982). In vitro and in vivo effector function of rat IgG2a monoclonal anti-S. mansoni antibodies. Journal of Immunology 129, 2739–43.
Hamburger, J., Lustigman, S., Arap-Siongok, T. K., Ouma, J. H. & Mahmoud, A. A. F. (1982). Characterization of a purified glycoprotein from Schistosoma mansoni eggs: specificity, stability, and the involvement of carbohydrate and peptide moieties in its serologic activity. Journal of Immunology 128, 1864–9.
Hamburger, J., Pelley, R. P. & Warren, K. S. (1976). Schistosoma mansoni soluble egg antigens: determination of the stage and species specificity of their serological reactivity by radioimmunoassay. Journal of Immunology 117, 1561–6.
Harn, D. A., Mitsuyama, M. & David, J. R. (1984). Schistosoma mansoni: anti-egg monoclonal antibodies protect against cercarial challenge in vivo. Journal of Experimental Medicine 159, 1371–87.
Harrison, D. J., Carter, C. E. & Colley, D. G. (1979). Immunoaffinity purification of Schistosoma mansoni soluble egg antigens. Journal of Immunology 122, 2210–17.
Hockley, D. J. & McLaren, D. J. (1973). Schistosoma mansoni: changes in the outer membrane of the tegument during development from cercariae to adult worm. International Journal for Parasitology 3, 1442–8.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the folin phenol reagent, Journal of Biological Chemistry 193, 265–75.
Miller, K. L., Smithers, R. S. & Sher, A. (1981). The response of mice immune to Schistosoma mansoni to a challenge infection which by-passes the skin: evidence of two mechanisms of immunity, Parasite Immunology 3, 2531.
Moser, G., Wassom, D. L. & Sher, A. (1980). Studies of the antibody-dependent killing of schistosomula of Schistosoma mansoni employing haptenic target antigens. I. Evidence that the loss in susceptibility to immune damage undergone by developing schistosomula involves a change unrelated to the masking of parasite antigens by host molecules. Journal of Experimental Medicine 152, 4153.
Murrell, K. D., Taylor, D. W., Vannier, W. E. & Dean, D. A. (1978). Schistosoma mansoni: analysis of surface membrane carbohydrates using lectins. Experimental Parasitology 46, 247–55.
Pearce, E. J., Basch, P. F. & Sher, A. (1986). Evidence that the reduced surface antigenicity of developing Schistosoma mansoni schistosomula is due to antigen shedding rather than host molecule acquisition. Parasite Immunology 8, 7994.
Pelley, R. R., Pelley, R. J., Hamburger, J., Peters, P. A. & Warren, K. S. (1976). Schistosoma mansoni soluble egg antigens. I. Identification and purification of three major antigens and the employment of radioimmunoassay for their further characterization. Journal of Immunology 117, 1553–66.
Sadun, E. H., Schoenbechler, M. J. & Bentz, M. (1965). Multiple antibody responses in Schistosoma mansoni infections: antigenic constituents in eggs, cercariae and adults (excretions and secretions) determined by flocculation reactions, cross absorption and double diffusion studies. American Journal of Tropical Medicine and Hygiene 14, 977–95.
Samuelson, J. C. & Caulfield, J. P. (1982). Loss of covalently labelled glycoproteins and glycolipids from the surface of newly transformed schistosomula of Schistosoma mansoni. Journal of Cell Biology 94, 363–9.
Samuelson, J. C., Caulfield, J. P. & David, J. R. (1982). Schistosomula of Schistosoma mansoni clear concanavalin A from their surface by sloughing. Journal of Cell Biology 94, 355–62.
Simpson, A. J. G., Correa-Oliveira, R., Smithers, S. R. & Sher, A. (1983). The exposed carbohydrates of schistosomula of Schistosoma mansoni and their modification during maturation in vivo. Molecular and Biochemical Parasitology 8, 191205.
Simpson, A. J. G., James, S. L. & Sher, A. (1983). Identification of surface antigens of schistosomula of Schistosoma mansoni recognized by antibodies from mice immunized by chronic infection and by exposure to highly irradiated cercariae. Infection and Immunity 41, 5967.
Simpson, A. J. G. & Smithers, S. R. (1985). Schistosomes: surface, egg and circulating antigens. Current Topics in Microbiology and Immunology 120, 205–39.
Smith, M. A., Clegg, J. A., Snary, D. & Trejdosiewicz, A. J. (1982). Passive immunization of mice against Schistosoma mansoni with an IgM monoclonal antibody. Parasitology 84, 83–9.
Smithers, S. R. & Williamson, J. (1961). Antigenic polysaccharide material in cercariae and eggs of Schistosoma mansoni. Transactions of the Royal Society of Tropical Medicine and Hygiene 55, 308–9.
Stein, P. C. & Lumsden, R. D. (1973). Schistosoma mansoni: topographical features of cercariae, schistosomula, and adults. Experimental Parasitology 33, 499514.
Tarrab-Hazdai, R., Levi-Schaffer, F., Brenner, V., Horowitz, S., Eshhar, Z. & Arnon, R. (1985). Protective monoclonal antibody against Schistosoma mansoni: antigen isolation, characterization, and suitability for active immunization. Journal of Immunology 135, 2772–9.
Zodda, D. M. & Phillips, S. M. (1982). Monoclonal antibody-mediated protection against Schistosoma mansoni infection in mice. Journal of Immunology 129, 2326–8.

Identification and characterization of a polysaccharide-containing antigen from Schistosoma mansoni eggs which cross-reacts with the surface of schistosomula

  • D. W. Dunne (a1) and Q. D. Bickle (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed