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Host serum modifies the drug susceptibility of Babesia bovis in vitro

  • S. BORK (a1), M. OKAMURA (a1), T. MATSUO (a1), S. KUMAR (a1), N. YOKOYAMA (a1) and I. IGARASHI (a1)...

Abstract

Babesia parasites generally require a defined percentage of serum in the culture medium for their in vitro growth. In this study, we attempted to culture Babesia bovis in a serum-free condition. The growth pattern and morphology of B. bovis in serum-free (plain) GIT medium were unaltered as compared to those of the standard growth condition containing 40% bovine serum in M199. When exposed to the test drugs, the parasite in plain GIT medium showed clearly lower IC50 values than those in 40% serum-containing GIT medium, indicating that several serum components may interfere with the drug bio-availability. Therefore, the serum-free culture system is useful for standardizing drug test protocols and understanding the roles of serum factors in the drug test.

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Tel: +81 155 49 5641. Fax: +81 155 49 5643. E-mail: igarcpmi@obihiro.ac.jp

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Keywords

Host serum modifies the drug susceptibility of Babesia bovis in vitro

  • S. BORK (a1), M. OKAMURA (a1), T. MATSUO (a1), S. KUMAR (a1), N. YOKOYAMA (a1) and I. IGARASHI (a1)...

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