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Host genetic influences on the anthelmintic efficacy of papaya-derived cysteine proteinases in mice

Published online by Cambridge University Press:  04 March 2015

WENCESLAUS LUOGA ,
FADLUL MANSUR ,
GILLIAN STEPEK ,
ANN LOWE ,
IAN R. DUCE ,
DAVID. J. BUTTLE  and
JERZY M. BEHNKE
WENCESLAUS LUOGA
Affiliation:
School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK Department of Life Sciences, Mkwawa University College of Education, Iringa, Tanzania
FADLUL MANSUR
Affiliation:
School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia (USIM), Kuala Lumpur, Malaysia
GILLIAN STEPEK
Affiliation:
School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
ANN LOWE
Affiliation:
School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
IAN R. DUCE
Affiliation:
School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
DAVID. J. BUTTLE
Affiliation:
Department of Infection and Immunity, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX, UK
JERZY M. BEHNKE*
Affiliation:
School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
*
*Correspondence author. School of Life Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK. E-mail: jerzy.behnke@nottingham.ac.uk
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Summary

Eight strains of mice, of contrasting genotypes, infected with Heligmosomoides bakeri were studied to determine whether the anthelmintic efficacy of papaya latex varied between inbred mouse strains and therefore whether there is an underlying genetic influence on the effectiveness of removing the intestinal nematode. Infected mice were treated with 330 nmol of crude papaya latex or with 240 nmol of papaya latex supernatant (PLS). Wide variation of response between different mouse strains was detected. Treatment was most effective in C3H (90·5–99·3% reduction in worm counts) and least effective in CD1 and BALB/c strains (36·0 and 40·5%, respectively). Cimetidine treatment did not improve anthelmintic efficacy of PLS in a poor drug responder mouse strain. Trypsin activity, pH and PLS activity did not differ significantly along the length of the gastro-intestinal (GI) tract between poor (BALB/c) and high (C3H) drug responder mouse strains. Our data indicate that there is a genetic component explaining between-mouse variation in the efficacy of a standard dose of PLS in removing worms, and therefore warrant some caution in developing this therapy for wider scale use in the livestock industry, and even in human medicine.

Keywords

geneticscysteine proteinasesanthelminticsnematodesHeligmosomoides bakeripapaya latex
Type
Research Article
Information
Parasitology , Volume 142 , Issue 7 , June 2015 , pp. 989 - 998
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Copyright
Copyright © Cambridge University Press 2015 

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Footnotes

†

Present address: Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Bearsden Road, Glasgow, G61 1QH, UK

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