Skip to main content Accessibility help
×
Home

A Gene associated with cell division and drug resistance in Giardia duodenalis

  • J. A. Upcroft (a1), A. Healey (a1), D. G. Murray (a1), P. F. L. Boreham (a1) and P. Upcroft (a1)...

Extract

A 3000 bp cDNA insert (G6/1) from Giardia duodenalis, cloned into Escherichia coli is located on chromosome 3 of Giardia stocks which have 3 chromosomes detectable by field-inversion gel electrophoresis in the range 650–800 kb and on chromosome 3 and/or 4 of stocks with 4 chromosomes in this size range. The loss of this sequence from chromosome 4 but not chromosome 3 was associated with the induction of drug resistance in a previously sensitive laboratory stock. G6/1 appears to represent a single copy gene in Giardia as determined by hybridization of the probe to cleaved genomic DNA. Furthermore, the sequences flanking at least 12 kb of G6/1 are the same when G6/1 appears on both chromosomes 3 and 4. The cDNA encodes a protein associated with the nuclei of trophozoites during some stages of growth of the parasite. In a non-dividing culture, the antigen is associated with the nuclei of about 30% of trophozoites and fewer in a dividing culture. Three Giardia stocks with obvious chromosome rearrangements, which grow poorly and fail to divide normally, apparently lack the DNA sequence G6/1.

Copyright

References

Hide All
AggarwalA., DE A., DE, LaCRUZ, V. F. CRUZ, V. F. & Nash, T. E. (1990). A heat shock protein gene in Giardia lamblia unrelated to HSP70. Nucleic Acids Research 18, 3409.
Aggarwal, A., Merritt, J. W. JR. & Nash, T. E. (1989). Cysteine-rich variant surface proteins of Giardia lamblia. Molecular and Biochemical Parasitology 32, 3948.
Boreham, P. F. L., Phillips, R. E. & Shepherd, R. W. (1988). Altered uptake of metronidazole in vitro by stocks of Giardia intestinalis with different drug sensitivities. Transactions of the Royal Society of Tropical Medicine and Hygiene 82, 104–6.
Capon, A. G., Upcroft, J. A., Boreham, P. F. L., Cottis, L. E. & Bundesen, P. G. (1989). Similarities of Giardia antigens derived from human and animal sources. International Journal for Parasitology 19, 91–8.
Clark, J. T. & Holberton, D. V. (1988). Triton-labile antigens in flagella isolated from Giardia lamblia. Parasitology Research 74, 415–23.
Feinberg, A. P. & Vogelstein, B. (1984). Addendum. ‘A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.’ Analytical Biochemistry 137, 266–7.
Gillin, F. D., Hagblom, P., Harwood, J., Aley, S. B., Reiner, D. S., Mccaffery, M., So, M. & Guiney, D. G. (1990). Isolation and expression of the gene for a major surface protein of Giardia lamblia. Proceedings of the National Academy of Sciences, USA 87, 4463–7.
Giloh, H. & Sedat, J. W. (1982). Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate. Science 217, 1252–5.
Hoyne, G. F., Boreham, P. F. L., Parsons, P. G., Ward, C. & Biggs, B. (1989). The effect of drugs on the cell cycle of Giardia intestinalis. Parasitology 99, 333–9.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 277, 680–5.
Lindley, T. A., Chakraborty, P. R. & Edlind, T. D. (1988). Heat shock and stress response in Giardia lamblia. Molecular and Biochemical Parasitology 28, 135–44.
Peattie, D. A., Alonso, R. A., Hein, A. & Caulfield, J. P. (1989). Ultrastructural localization of giardins to the edges of disk microribbons of Giardia lamblia and the nucleotide and deduced protein sequence of alpha giardin. Journal of Cell Biology 109, 2323–35.
Phillips, R. E., Boreham, P. F. L. & Shepherd, R. W. (1984). Cryopreservation of viable Giardia intestinalis trophozoites. Transactions of the Royal Society for Tropical Health and Hygiene 78, 604–6.
Southern, E. M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98, 503–17.
Upcroft, J. A., Boreham, P. F. L. & Upcroft, P. (1989 a). Geographic variation in Giardia karyotypes. International Journal for Parasitology 19, 519–27.
Upcroft, J. A., Boreham, P. F. L. & Upcroft, P. (1989 c). Molecular mechanisms of drug resistance in Giardia intestinalis. In Immunological Basis of Pathogenesis in Parasitic Diseases (ed. Ko, R. C.), pp. 141153. Hong Kong: University of Hong Kong Press.
Upcroft, J. A., Capon, A. G., Dharmkrong-At, A., Healey, A., Boreham, P. F. L. & Upcroft, P. (1987). Giardia intestinalis antigens expressed in Escherichia coli. Molecular and Biochemical Parasitology 26, 267–76.
Upcroft, J. A., Dunn, L. A., Dommett, L. S., Upcroft, P. & Boreham, P. F. L. (1989 b). Chromosomes of Blastocystis hominis. International Journal for Parasitology 19, 879–83.
Upcroft, J. A., Healey, A., Mitchell, R., Boreham, P. F. L. & Upcroft, P. (1990 a). Antigen expression from the ribosomal DNA repeat unit of Giardia intestinalis. Nucleic Acids Research 18, 7077–81.
Upcroft, J. A., Upcroft, P. & Boreham, P. F. L. (1990 b). Drug resistance in Giardia intestinalis. International Journal for Parasitology 20, 489–96.
Upcroft, P. (1991). DNA fingerprinting of the human intestinal parasite Giardia intestinalis with hypervariable minisatellite sequences. In DNA Fingerprinting: Approaches and Applications (ed Burke, T., Dolf, G., Jeffreys, A. J. & Wolff, R.), pp. 7684. Basel: Birkhäuser Verlag.
Upcroft, P., Boreham, P. F. L. & Upcroft, J. A. (1989 d). Genetic variation in Giardia intestinalis in comparison with other unicellular organisms. In Immunological Basis of Pathogenesis in Parasitic Diseases (ed Ko, R. C.), pp. 155168. Hong Kong: University of Hong Kong Press.
Upcroft, P. & Healey, A. (1987). Rapid and efficient method for cloning of blunt-ended DNA fragments. Gene 51, 6975.
Upcroft, P., Mitchell, R. & Boreham, P. F. L. (1990 c). DNA fingerprinting of the intestinal parasite Giardia duodenalis with the M13 phage genome. International Journal for Parasitology 20, 319–23.

Keywords

A Gene associated with cell division and drug resistance in Giardia duodenalis

  • J. A. Upcroft (a1), A. Healey (a1), D. G. Murray (a1), P. F. L. Boreham (a1) and P. Upcroft (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed