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Evaluation of immune response to Bacillus subtilis spores expressing Clonorchis sinensis serpin3

Published online by Cambridge University Press:  14 May 2020

Zhipeng Lin ,
Hengchang Sun Open the ORCID record for Hengchang Sun [Opens in a new window] ,
Yan Ma ,
Xinyi Zhou ,
Hongye Jiang ,
Xi Wang ,
Jiaman Song ,
Zeli Tang ,
Qing Bian  and
Zhen Zhang
...Show all authors
Zhipeng Lin
Affiliation:
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou510080, People's Republic of China Key Laboratory for Tropical Diseases Control, Ministry of Education, Sun Yat-Sen University, Guangzhou510080, People's Republic of China
Hengchang Sun
Affiliation:
Department of laboratory medicine, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou510080, People's Republic of China
Yan Ma
Affiliation:
Department of respiratory medicine, Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai519099, People's Republic of China
Xinyi Zhou
Affiliation:
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou510080, People's Republic of China Key Laboratory for Tropical Diseases Control, Ministry of Education, Sun Yat-Sen University, Guangzhou510080, People's Republic of China
Hongye Jiang
Affiliation:
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou510080, People's Republic of China Key Laboratory for Tropical Diseases Control, Ministry of Education, Sun Yat-Sen University, Guangzhou510080, People's Republic of China
Xi Wang
Affiliation:
Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou510080, People's Republic of China
Jiaman Song
Affiliation:
Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou510080, People's Republic of China
Zeli Tang
Affiliation:
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou510080, People's Republic of China Key Laboratory for Tropical Diseases Control, Ministry of Education, Sun Yat-Sen University, Guangzhou510080, People's Republic of China Department of Cell Biology and Genetics, School of Pre-clinical Medicine, Guangxi Medical University, Nanning530021, People's Republic of China
Qing Bian
Affiliation:
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou510080, People's Republic of China Key Laboratory for Tropical Diseases Control, Ministry of Education, Sun Yat-Sen University, Guangzhou510080, People's Republic of China
Zhen Zhang
Affiliation:
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou510080, People's Republic of China Key Laboratory for Tropical Diseases Control, Ministry of Education, Sun Yat-Sen University, Guangzhou510080, People's Republic of China
Yan Huang*
Affiliation:
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou510080, People's Republic of China Key Laboratory for Tropical Diseases Control, Ministry of Education, Sun Yat-Sen University, Guangzhou510080, People's Republic of China
Xinbing Yu*
Affiliation:
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou510080, People's Republic of China Key Laboratory for Tropical Diseases Control, Ministry of Education, Sun Yat-Sen University, Guangzhou510080, People's Republic of China
*
Author for correspondence: Xinbing Yu, E-mail: yuxb@mail.sysu.edu, Yan Huang, E-mail: huang66@mail.sysu.edu.cn
Author for correspondence: Xinbing Yu, E-mail: yuxb@mail.sysu.edu, Yan Huang, E-mail: huang66@mail.sysu.edu.cn
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Abstract

Clonorchis sinensis (C. sinensis) is one of the most serious food-borne parasites, which can lead to liver fibrosis or cholangiocarcinoma. Effective measures for clonorchiasis prevention are still urgently needed. Bacillus subtilis (B. subtilis) is an effective antigen delivery platform for oral vaccines. Chonorchis sinensis serpin (CsSerpin) was proved to be potential vaccine candidates. In this study, CsSerpin3 was displayed on the surface of B. subtilis spore and recombinant spores were orally administrated to BALB/C mice. CsSerpin3-specific IgA levels in faecal, bile and intestinal mucous increased at 4–8 weeks after the first administration compared with those in control groups. The mucus production and the number of goblet cells in intestinal mucosa elevated in B.s-CotC-CsSerpin3 (CotC, coat protein of B. subtilis spore) spores treated group compared to those in blank control. No significant difference in the activities of glutamic-pyruvic transaminase/ alanine aminotransferase and glutamic oxalacetic transaminase/aspartate aminotransferase were observed between groups. There was no side effect inflammation and observable pathological damage in the liver tissue of mice after administration. Moreover, collagen deposition and Ishak score were statistically reduced in B.s-CotC-CsSerpin3 spores treated mice. In conclusion, B. subtilis spores displaying CsSerpin3 could be investigated further as an oral vaccine against clonorchiasis.

Keywords

Bacillus subtilisClonorchis sinensissporeSerpin3oral vaccine
Type
Research Article
Information
Parasitology , Volume 147 , Issue 10 , September 2020 , pp. 1080 - 1087
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to this work.

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