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The effects of combining Artemisia annua and Curcuma longa ethanolic extracts in broilers challenged with infective oocysts of Eimeria acervulina and E. maxima



Due to an increasing demand for natural products to control coccidiosis in broilers, we investigated the effects of supplementing a combination of ethanolic extracts of Artemisia annua and Curcuma longa in drinking water. Three different dosages of this herbal mixture were compared with a negative control (uninfected), a positive control (infected and untreated), chemical coccidiostats (nicarbazin+narazin and, later, salinomycin), vaccination, and a product based on oregano. Differences in performance (weight gain, feed intake, and feed conversion rate), mortality, gross intestinal lesions and oocyst excretion were investigated. Broilers given chemical coccidiostats performed better than all other groups. Broilers given the two highest dosages of the herbal mixture had intermediate lesion scores caused by Eimeria acervulina, which was higher than in broilers given coccidiostats, but less than in broilers given vaccination, oregano and in negative controls. There was a trend for lower mortality (P = 0·08) in the later stage of the growing period (23–43 days) in broilers given the highest dosage of herbal mixture compared with broilers given chemical coccidiostats. In conclusion, the delivery strategy of the herbal extracts is easy to implement at farm level, but further studies on dose levels and modes of action are needed.


Corresponding author

* Corresponding author: Department of Agroecology, Faculty of Sciences and Technology, Aarhus University. Research Centre Foulum, Blichers Allé 20, P.O. Box 50, DK-8830 Tjele, Denmark. E-mail:;


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Abbas, R. Z., Colwell, D. D. and Gilleard, J. (2012). Botanicals: an alternative approach for the control of avian coccidiosis. World's Poultry Science Journal 68, 203215. doi: 10.1017/S0043933912000268.
Akhtara, M., Haia, A., Awaisa, M. M., Iqbala, Z., Muhammadb, F., Haqc, A. U. and Anward, M. I. (2012). Immunostimulatory and protective effects of Aloe vera against coccidiosis in industrial broiler chickens. Veterinary Parasitology 186, 170177.
Allen, P. C., Lydon, J. and Danforth, H. D. (1997). Effects of components of Artemisia annua on coccidia infections in chickens. Poultry Science 76, 11561163.
Allen, P. C., Danforth, H. D. and Augustine, P. C. (1998). Dietary modulation of avian coccidiosis. International Journal for Parasitology 8, 11311140.
Almeida, G. F d., Horsted, K., Thamsborg, S. M., Kyvsgaard, N. C., Ferreira, J. F. S. and Hermansen, J. E. (2012). Use of Artemisia annua as a natural coccidiostat in free-range broilers and its effects on infection dynamics and performance. Veterinary Parasitology 186, 178187.
Anonymous (1990). Diagnosis of Coccidiosis in Chickens. Janssen Pharmaceutica, Beerse, Belgium.
Anwar, M. I., Akhtar, M., Hussain, I., Muhammad, F. and Haq, A. U. (2008). Effects of local gametocyte and livacox vaccines on live body weight gain and lymphoid organs in chickens. Pakistan Veterinary Journal 28, 136138.
Arab, H. A., Rahbari, S., Rassouli, A., Moslemi, M. H. and Khosravirad, F. D. A. (2006). Determination of artemisinin in Artemisia sieberi and anticoccidial effects of the plant extract in broiler chickens. Tropical Animal Health and Production 38, 497503.
Chapman, H. D. (1993). Resistance to anticoccidial drugs in fowl. Parasitology Today 9, 159162.
Conney, A. H., Lysz, T., Ferraro, T., Abidi, T. F., Manchand, P. S., Laskin, J. D. and Huang, M. T. (1991). Inhibitory effect of curcumin and some related dietary compounds on tumor promotion and arachidonic metabolism in mouse skin. Advances in Enzyme Regulation 31, 385396.
Conway, D. P., Sasai, A. K., Gaafar, B. S. M. and Smothers, C. D. (2003). Effects of different levels of oocyst inocula of Eimeria acervulina, E. tenella, and E. maxima on plasma constituents, packed cell volume, lesion scores, and performance in chickens. Avian Diseases 37, 118123.
Crane, M. S., Murray, P. K., Gnozzio, M. J. and MacDonald, T. T. (1988). Passive protection of chickens against Eimeria tenella infection by monoclonal antibody. Infection and Immunity 56, 972976.
Cui, L., Miao, J. and Cui, L. (2007). Cytotoxic effect of curcumin on malaria parasite Plasmodium falciparum: inhibition of histone acetylation and generation of reactive oxygen species. Antimicrobial Agents and Chemotherapy 51, 488494.
Daugschies, A., Gässlein, U. and Rommel, M. (1998). Comparative efficacy of anticoccidials under the conditions of commercial broiler production and in battery trials. Veterinary Parasitology 76, 163171.
Eckert, J., Taylor, M., Catchpole, J., Licois, D., Coudert, P. and Bucklar, H. (1995). Morphological characteristics of oocysts. In Biotechnology. Guidelines of Techniques in Coccidiosis Research (ed. Eckert, J., Braun, R., Shirley, M. and Coudert, P.), pp. 103119. COST 89/820. Office for Official Publications of the European Communities, Luxembourg.
Ferreira, J. F. S. and Gonzales, J. M. (2009). Analysis of underivatized artemisinin and related sesquiterpene lactones by high-performance liquid chromatography with ultraviolet detection. Phytochemical Analysis 2, 9197.
Ferreira, J. F. S., Peaden, P. and Keiser, J. (2011). In vitro trematocidal effects of crude alcohol-ic extracts of Artemisia annua, A. absinthium, Asimina triloba, and Fumaria officinalis . Parasitology Research 109, 15851592.
Hafeez, M. A., Akhtar, M., Javed, M. T. and Haq, A. U. (2007). Maternal immunization by egg propagated gametocyte vaccine to control Eimeria tenella infections in newly hatched chicks. Parasitology Research 100, 11391141.
Henken, A. M., Ploeger, H. W., Graat, E. A. M. and Carpenter, T. E. (1994). Description of a simulation model for the population dynamics of Eimeria acervulina infection in broilers. Parasitology 108, 503512.
Huang, M. T., Newmark, H. L. and Frenkel, K. (1997). Inhibitory effects of curcumin on tumorigenesis in mice. Journal of Cellular Biochemistry Supplement 27, 2634.
Isacchi, B., Bergonzi, M. C., Grazioso, M., Righeschi, C., Pietretti, A., Severini, C. and Bilia, A. R. (2012). Artemisinin and artemisinin plus curcumin liposomal formulations: enhanced antimalarial efficacy against Plasmodium berghei-infected mice. European Journal of Pharmaceutics and Biopharmaceutics 80, 528534.
Johnson, J. and Reid, W. M. (1970). Anticoccidial drugs lesion scoring techniques in battery and floor-pen experiments with chickens. Experimental Parasitology 28, 3036.
Jung, M., Lee, K., Kim, H. and Park, M. (2004). Recent advances in artemisinin and its derivatives as antimalarial and antitumor agents. Current Medical Chemistry 11, 12651284.
Lillehoj, H. S. and Trout, J. M. (1993). Coccidia: a review of recent advances on immunity and vaccine development. Avian Pathology 22, 331.
Martin, A., Danforth, H., Barta, J. and Fernando, M. (1997). Analysis of immunological cross-protection and sensitivities to anticoccidial drugs among five geographical and temporal strains of Eimeria maxima . International Journal for Parasitology 27, 527533.
Mathis, G. F. (2005). Reasons for field problems with E. maxima: E. acervulina vs E. maxima. In Proceeding of the IXth International Coccidiosis Conference, Foz do Iguacú, Brazil. 19–23 Sept 2005.
McDougald, L. R., Fuller, L. and Mattiello, R. (1997). A survey of coccidian on 43 poultry farms in Argentina. Avian Diseases 41, 923929.
Naidoo, V., McGaw, L. J., Bisschop, S. P. R., Duncan, N. and Eloff, J. N. (2008). The value of plant extracts with antioxidant activity in attenuating coccidiosis in broiler chickens. Veterinary Parasitology 153, 214219.
Nandakumar, D. N., Nagaraj, V. A., Vathsala, P. G., Rangarajan, P. and Padmanaban, G. (2006). Curcumin-artemisinin combination therapy for malaria. Antimicrobial Agents and Chemotherapy 50, 18591860.
Orengo, J., Buendía, A. J., Ruiz-Ibáñez, M. R., Madrid, J., Del Río, L., Catalá-Gregori, P., García, V. and Hernández, F. (2012). Evaluating the efficacy of cinnamaldehyde and Echinacea purpurea plant extract in broilers against Eimeria acervulina . Veterinary Parasitology 185, 158163.
Oviedo-Rondón, E. O., Clemente-Hernández, S., Salvador, F., Williams, P., Losa, R. and Stephen, F. (2006). Essential oils on mixed coccidia vaccination and infection in broilers. International Journal of Poultry Science 5, 723730.
Padmanaban, G., Nagaraj, V. A. and Rangarajan, P. N. (2012). Artemisinin-based combination with curcumin adds a new dimension to malaria therapy. Current Science 102, 704711.
Pinard-van der Laan, M.-H., Monvoisin, J.-L., Pery, P., Hamet, N. and Thomas, M. (1998). Comparison of outbred lines of chickens for resistance to experimental infection with coccidiosis (Eimeria tenella). Poultry Science 77, 185191.
Quinn, G. P. and Keough, M. J. (2002). Experimental Design and Data Analysis for Biologists. 6th printing, 2007. Cambridge University Press, Cambridge, UK.
Rao, C. V., Rivenson, A., Simi, B. and Reddy, B. S. (1995). Chemoprevention of colon carcinogenesis by dietary curcumin, a naturally occurring plant phenolic compound. Cancer Research 55, 259266.
Reddy, R. C., Vathsala, P. G., Keshamouni, V. G., Padmanaban, G. and Rangarajan, P. N. (2005). Curcumin for malaria therapy. Biochemical and Biophysical Research Communications 326, 472474.
Rosen, G. D. (1995). Antibacterials in poultry and pig nutrition. In Biotechnology in Animal Feeds and Animal Feeding (ed. Wallace, R. J. and Chesson, A.), pp. 143172. VCH Verlagsgesellschaft GmbH, Weinheim, Germany.
SAS Institute (2000). SAS Online Doc®, Version 9.2. SAS Institute, Cary, NC.
Shahiduzzaman, M., Dyachenko, V., Khalafalla, R. E., Desouky, A. Y. and Daugschies, A. (2009). Effects of curcumin on Cryptosporidium parvum in vitro . Parasitology Research 105, 11551161.
Shirley, M. W., Smith, A. L. and Tomley, F. M. (2005). The biology of avian Eimeria with an emphasis on their control by vaccination. Advanced Parasitology 60, 285330.
Souza, C. R. A. and Glória, M. B. A. (1998). Chemical analysis of turmeric from Minas Gerais, Brazil and comparison of methods for flavour free oleoresin. Brazilian Archives of Biology and Technology 41, 218224.
Subramanian, M., Sreejayan Rao, M. N., Devasagayam, T. P. and Singh, B. B. (1994). Diminution of singlet oxygen-induced DNA damage by curcumin and related antioxidants. Mutation Research 311, 249255.
Velkers, F. C., Swinkels, W. J. C., Rebel, J. M. J., Bouma, A., Daemen, A. J. J. M., Klinkenberg, D., Boersma, W. J. A., Stegeman, J. A., de Jong, M. C. M. and Heesterbeek, J. A. P. (2010). Effect of Eimeria acervulina infection history on the immune response and transmission in broilers. Veterinary Parasitology 173, 184192.
Waldenstedt, L. (2003). Effect of vaccination against coccidiosis in combination with an antibacterial oregano (Origanum vulgare) compound in organic broiler production. Acta Agriculturae Scandinavica 53, 101109.
Williams, R. B. (1999). A compartmentalized model for the estimation of the cost of coccidiosis to the world's chicken production industry. International Journal for Parasitology 8, 12091229.
Williams, R. B. (2002). Fifty years of anticoccidial vaccines for poultry, 1952–2002. Avian Disease 46, 755802.


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The effects of combining Artemisia annua and Curcuma longa ethanolic extracts in broilers challenged with infective oocysts of Eimeria acervulina and E. maxima



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