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The effect of the sulphoxide metabolite of triclabendazole (‘Fasinex’) on the tegument of mature and immature stages of the liver fluke, Fasciola hepatica

Published online by Cambridge University Press:  06 April 2009

A. W. Stitt  and
I. Fairweather
A. W. Stitt
Affiliation:
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN
I. Fairweather
Affiliation:
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN
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Summary

The effects of the novel benzimidazole, triclabendazole (TCBZ) (‘Fasinex’, Ciba-Geigy), in its active sulphoxide metabolite form (TCBZ-SX), on the tegumental ultrastructure of Fasciola hepatica were determined in vitro by transmission electron microscopy (TEM), using both intact flukes and tissue-slice material. At a concentration of 15 µ/ml, the tegument of the whole adult fluke showed ultrastructural changes only after prolonged time-periods, with vacuolation at the base of the syncytium and accumulation of T2 secretory bodies in the tegumental cells. At a concentration of 50 µ/ml, with both whole flukes and tissue-slices, the tegument appeared extremely abnormal with accumulation of secretory bodies towards the base of the syncytium. With longer incubation times, the tegument was completely sloughed away and the tegumental cells became synthetically inactive. The tegument of the 3-week-old juvenile became progressively convoluted at the apex, while in the basal regions there was severe vacuolation. In the tegumental cells, there were accumulations of T1 secretory bodies. These results confirm TCBZ as a potent fasciolicide, being very effective in disrupting the fluke tegument. They may go some way to explain the mode of action of this important fasciolicide.

Keywords

Fasciola hepaticaliver fluketegumental ultrastructuresulphoxide metabolite of triclabendazole (TCBZSX)transmission electron microscopyin vitro culture
Type
Research Article
Information
Parasitology , Volume 108 , Issue 5 , June 1994 , pp. 555 - 567
Copyright
Copyright © Cambridge University Press 1994

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