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The effect of proteases and iodination on the adherent behaviour of Plasmodium falciparum-infected erythrocytes

Published online by Cambridge University Press:  06 April 2009

I. Crandall ,
D. Demers  and
I. W. Sherman
I. Crandall
Affiliation:
Department of Biology, University of California, Riverside, CA 92521, USA
D. Demers
Affiliation:
Department of Biology, University of California, Riverside, CA 92521, USA
I. W. Sherman*
Affiliation:
Department of Biology, University of California, Riverside, CA 92521, USA
*
†Corresponding author. Tel: 909 787 5905. Fax: 909 787 4286. E-mail: Sherman@mail.ucr.edu.
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Summary

Plasmodium falciparum-infected erythrocytes were treated with proteases (trypsin, chymotrypsin, pronase, or V8 protease) or iodinated and the effect of these treatments on the cytoadherent behaviour of the cells was determined. As previously observed, protease treatment reduced cytoadherence. However, it was also found that the P. falciparum-induced adhesin, pfalhesin, was not removed by protease treatment. Gelatin flotation experiments and scanning electron microscopical examination of the treated cells indicated that protease exposure resulted in changes in the knob structures on the cells, which are known to affect the adherent behaviour of the cells. Iodination was found to be an effective method of inactivating pfalhesin.

Keywords

Plasmodium falciparumcytoadherenceprotease treatmentmalariaknobs.
Type
Research Article
Information
Parasitology , Volume 113 , Issue 4 , October 1996 , pp. 317 - 322
Copyright
Copyright © Cambridge University Press 1996

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