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Echinococcus granulosus: in vitro effect of monensin on the tegument of the protoscolex

Published online by Cambridge University Press:  06 April 2009

M. T. Rogan  and
K. Sylvia Richards
M. T. Rogan
Affiliation:
Parasitology Research Laboratory, Department of Biological Sciences, University of Keele, Keele, Staffs. ST5 5BG
K. Sylvia Richards
Affiliation:
Parasitology Research Laboratory, Department of Biological Sciences, University of Keele, Keele, Staffs. ST5 5BG
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Summary

Protoscoleces of Echinococcus granulosus were incubated in a 10 μm solution of monensin and their viability and the ultrastructure of their tegument were monitored over a 36 h period. The earliest effects of the lonophore, apparent within 15 min, involved a degree of cisternal swelling of the tegumentary Golgi complexes. Swelling became more pronounced with time and cytons eventually contained numerous large electron-lucent vesicles. Residual bodies indicative of autophagy were observed in the cytons and distal cytoplasm from 1 h onwards. All movement of protoscoleces ceased after 1 h and the surface of the soma subsequently became folded, possibly suggesting spastic paralysis. Later, vacuoles and large cytoplasmic blebs were observed in the distal cytoplasm and viability dropped rapidly after 12 h. All protoscoleces were dead at 36 h. The potential of monensin as an in vivo protoscolicidal agent is discussed.

Type
Research Article
Information
Parasitology , Volume 93 , Issue 2 , October 1986 , pp. 347 - 355
Copyright
Copyright © Cambridge University Press 1986

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