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Different mechanisms of transmission of the microsporidium Octosporea bayeri: a cocktail of solutions for the problem of parasite permanence

  • D. B. VIZOSO (a1) (a2), S. LASS (a1) and D. EBERT (a1)


Periods of low host density impose a constraint on parasites with direct transmission, challenging their permanence in the system. The microsporidium Octosporea bayeri faces such constraint in a metapopulation of its host, the cladoceran Daphnia magna, where ponds frequently lose their host population due to ponds drying out in summer and freezing in winter. We conducted experiments aimed to investigate the mechanisms of transmission of O. bayeri, and discuss how these mechanisms could contribute to the parasite's permanence in the system. Spores accumulate in the fat cells and the ovaries of the host, and vary in morphology, possibly corresponding to 3 different spore types. Horizontal transmission occurred through the release of spores from dead hosts, with the proportion of infected hosts depending on the spore dose. Further, spores are able to persist outside the host both in dry and wet conditions. Vertical transmission occurred to both parthenogenetic and sexual offspring. The former were invariably infected, while the sexually produced resting eggs (=ephippia) had a less efficient transmission. The parasite may be carried by the ephippia, and thus disperse to new ponds together with the host. Together, these mechanisms may allow the parasite to endure periods of harsh environmental conditions both outside and inside the host.


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AGNEW, P., BECNEL, J. J., EBERT, D. & MICHALAKIS, Y. ( 2002). Symbiosis of microsporidia and insects. In Insect Symbiosis (ed. Bourtzis, K.), pp. 145163. CRC Press LLC, Florida.
ANDERSON, R. M. & MAY, R. M. ( 1978). Regulation and stability of host-parasite population interactions.1. Regulatory processes. Journal of Animal Ecology 47, 219247.
ANDERSON, R. M. & MAY, R. M. ( 1981). The population dynamics of micro-parasites and their invertebrate hosts. Philosophical Transactions of the Royal Society of London, Series B 291, 451524.
ANDERSON, R. M. & MAY, R. M. ( 1986). The invasion, persistence and spread of infectious-diseases within animal and plant-communities. Philosophical Transactions of the Royal Society of London, Series B 314, 533570.
BECNEL, J. J. & ANDREADIS, T. G. ( 1999). Microsporidia in insects. In The Microsporidia and Microsporidiosis (ed. Wittner, M. & Weiss, L. M.), pp. 447501. American Society for Microbiology, Washington, D.C.
BIGLIARDI, E. & SACCHI, L. ( 2001). Cell biology and invasion of the Microsporidia. Microbes and Infection 3, 373379.
BONHOEFFER, S., LENSKI, R. E. & EBERT, D. ( 1996). The curse of the pharaoh: The evolution of virulence in pathogens with long living propagules. Proceedings of the Royal Society of London, Series B 263, 715721.
BROOKS, W. M. ( 1988). Entomogenous protozoa. In CRC Handbook of Natural Pesticides. Microbial Insecticides, Part A: Entomogenous Protozoa and Fungi (ed. Ignoffo, C. M.), pp. 1150. CRC Press, Boca Raton, Florida.
BULL, J. J., MOLINEUX, I. J. & RICE, W. R. ( 1991). Selection of benevolence in a host-parasite system. Evolution 45, 875882.
CANNING, E. ( 1982). An evaluation of protozoal characteristics in relation to biological control of pests. Parasitology 84, 119149.
CANNING, E. U., REFARDT, D., VOSSBRINCK, C. R., OKAMURA, B. & CURRY, A. ( 2002). New diplokaryotic microsporidia (Phylum Microsporidia) from freshwater bryozoans (Bryozoa, Phylactolaemata). European Journal of Protistology 38, 247265.
DUNN, A. M. & SMITH, J. E. ( 2001). Microsporidian life cycles and diversity: the relationship between virulence and transmission. Microbes and Infection 3, 381388.
EBERT, D. ( 1995). The ecological interactions between a microsporidian parasite and its host Daphnia magna. Journal of Animal Ecology 64, 361369.
EBERT, D., HOTTINGER, J. W. & PAJUNEN, V. I. ( 2001). Temporal and spatial dynamics of parasite richness in a Daphnia metapopulation. Ecology 82, 34173434.
EBERT, D., ZSCHOKKE-ROHRINGER, C. D. & CARIUS, H. J. ( 1998). Within- and between-population variation for resistance of Daphnia magna to the bacterial endoparasite Pasteuria ramosa. Proceedings of the Royal Society of London, Series B 265, 21272134.
FRANK, S. A. ( 1991). Ecological and genetic models of host-pathogen coevolution. Heredity 67, 7383.
FRANK, S. A. ( 1996). Models of parasite virulence. Quarterly Review of Biology 71, 3778.
GATEHOUSE, H. S. & MALONE, L. A. ( 1998). The ribosomal RNA gene region of Nosema apis (Microspora): DNA sequence for small and large subunit rRNA genes and evidence of a large tandem repeat unit size. Journal of Invertebrate Pathology 71, 97105.
GREEN, J. ( 1957). Parasites and epibionts of Cladocera in rock pools of Tvärminne archipelago. Archivum Societatis Zoologicae Botanicae Fennicae “Vanamo” 12, 512.
GREEN, J. ( 1974). Parasites and epibionts of Cladocera. Transactions of the Zoological Society of London 32, 417515.
HAAG, C. R., HOTTINGER, J. W., RIEK, M. & EBERT, D. ( 2002). Strong inbreeding depression in a Daphnia metapopulation. Evolution 56, 518526.
JÍROVEC, O. ( 1936). Über einige in Daphnia magna parasitierende Mikrosporidien. Zoologischer Anzeiger 116, 136142.
KLEINBAUM, D. G., KUPPER, L. L., MULLER, K. E. & NIZAM, A. ( 1998). Applied regression Analysis and Other Multivariate Methods. Duxbury Press, Pacific Grove, CA.
KLÜTTGEN, B., DÜLMER, U., ENGELS, M. & RATTE, H. T. ( 1994). ADaM, an artificial freshwater for the culture of zooplankton. Water Research 28, 743746.
LUCAROTTI, C. J. & ANDREADIS, T. G. ( 1995). Reproductive strategies and adaptations for survival among obligatory microsporidian and fungal parasites of mosquitoes: a comparative analysis of Amblyospora and Coelomyces. Journal of the American Mosquito Control Association 11, 111121.
MADDOX, J. V. ( 2002). Environmental persistence of Microsporidia. In Factors Affecting the Survival of Entomopathogens (ed. Baur, M. E. & Fuxa, J. R.). Southern Cooperative Series Bulletin 400. Available at:
ONSTAD, D. W., MADDOX, J. V., COX, D. J. & KORNKVEN, E. A. ( 1990). Spatial and temporal dynamics of animals and the host-density threshold in epizootiology. Journal of Invertebrate Pathology 55, 7684.
PAJUNEN, V. I. ( 1986). Distributional dynamics of Daphnia species in a rock-pool environment. Annales Zoologici Fennici 23, 131140.
RANTA, E. ( 1979). Niche of Daphnia species in rock pools. Archiv für Hydrobiologie 87, 205223.
REGOES, R. R., HOTTINGER, J. W., SYGNARSKI, L. & EBERT, D. ( 2003). The infection rate of Daphnia magna by Pasteuria ramosa conforms with the mass-action principle. Epidemiology and Infection 131, 957966.
SAUVAGE, F., LANGLAIS, M., YOCCOZ, N. G. & PONTIER, D. ( 2003). Modelling hantavirus in fluctuating populations of bank voles: the role of indirect transmission on virus persistence. Journal of Animal Ecology 72, 113.
SMITH, J. E. & DUNN, A. M. ( 1991). Transovarial transmission. Parasitology Today 7, 146148.
VAN BAALEN, M. ( 2000). Parent-to-offspring infection and the struggle for transmission. In Evolutionary Biology of Host–Parasite Relationships: Theory Meets Reality (ed. Poulin, R., Moran, S. & Skorping, A.), pp. 97116. Elsevier, Amsterdam.
VIZOSO, D. B. & EBERT, D. ( 2004). Within-host dynamics of a microsporidium with horizontal and vertical transmission: Octosporea bayeri in Daphnia magna. Parasitology 128, 3138.
ZHU, X., WITTNER, M., TANOWITZ, H. B., CALI, A. & WEISS, L. M. ( 1993). Nucleotide sequence of the small ribosomal RNA of Encephalitozoon cuniculi. Nucleic Acids Research 21, 1315.


Different mechanisms of transmission of the microsporidium Octosporea bayeri: a cocktail of solutions for the problem of parasite permanence

  • D. B. VIZOSO (a1) (a2), S. LASS (a1) and D. EBERT (a1)


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