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Differences and similarities between Trichinella spiralis and T. pseudospiralis in morphology of stichocyte granules, peptide maps of excretory and secretory (E–S) products and messenger RNA of stichosomal glycoproteins

Published online by Cambridge University Press:  01 January 1998

Z. WU ,
I. NAGANO  and
Y. TAKAHASHI
Z. WU
Affiliation:
Department of Parasitology, Gifu University School of Medicine, Tsukasa 40 Gifu, 500 Japan
I. NAGANO
Affiliation:
Department of Parasitology, Gifu University School of Medicine, Tsukasa 40 Gifu, 500 Japan
Y. TAKAHASHI
Affiliation:
Department of Parasitology, Gifu University School of Medicine, Tsukasa 40 Gifu, 500 Japan
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Abstract

The ultrastructure of muscle larvae of Trichinella pseudospiralis was studied by electron microscopy. The overall structure of muscle larvae of T. pseudospiralis resembled that of T. spiralis except for the stichocyte granules. T. pseudospiralis had at least 3 kinds of stichocyte granules distinguishable from each other by their shape, size and inclusions. The granules had some resemblance to α granules or β granules of T. spiralis, but no resemblance to γ granules. In favour of these morphological differences and similarities among T. spiralis and T. pseudospiralis, excretory and secretory (E–S) products (originating from stichocyte granules) of the 2 species differed to some degree. In an analysis by 2-dimensional electrophoresis, some peptide spots migrating at 45 kDa were shared by the 2 species but the other spots were unique to each of the 2 species. Messenger RNA encoding the 43 kDa glycoprotein of stichocyte granules was detected in the muscle larvae of both species but mRNA encoding the 53 kDa glycoprotein was detected only in muscle larvae of T. spiralis.

Keywords

Trichinella spiralisTrichinella pseudospiralisstichocyte granulesE–S products
Type
Research Article
Information
Parasitology , Volume 116 , Issue 1 , January 1998 , pp. 61 - 66
Copyright
1998 Cambridge University Press

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