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Comparing parasitological vs serological determination of Schistosoma haematobium infection prevalence in preschool and primary school-aged children: implications for control programmes

  • WELCOME M. WAMI (a1) (a2), NORMAN NAUSCH (a1) (a2), KATHARINA BAUER (a1) (a2), NICHOLAS MIDZI (a3), REGGIS GWISAI (a4), PETER SIMMONDS (a2), TAKAFIRA MDULUZA (a5), MARK WOOLHOUSE (a2) and FRANCISCA MUTAPI (a1) (a2)...

Summary

To combat schistosomiasis, the World Health Organization (WHO) recommends that infection levels are determined prior to designing and implementing control programmes, as the treatment regimens depend on the population infection prevalence. However, the sensitivity of the parasitological infection diagnostic method is less reliable when infection levels are low. The aim of this study was to compare levels of Schistosoma haematobium infection obtained by the parasitological method vs serological technique. Infection levels in preschool and primary school-aged children and their implications for control programmes were also investigated. Infection prevalence based on serology was significantly higher compared with that based on parasitology for both age groups. The difference between infection levels obtained using the two methods increased with age. Consequentially, in line with the WHO guidelines, the serological method suggested a more frequent treatment regimen for this population compared with that implied by the parasitological method. These findings highlighted the presence of infection in children aged ⩽5 years, further reiterating the need for their inclusion in control programmes. Furthermore, this study demonstrated the importance of using sensitive diagnostic methods as this has implications on the required intervention controls for the population.

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The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution licence http://creativecommons.org/licenses/by/3.0/.

Corresponding author

*Corresponding author: Institute of Immunology & Infection Research, School of Biological Sciences, University of Edinburgh, Ashworth, Laboratories, King's Buildings, West Mains Rd, Edinburgh EH9 3JT, UK. E-mail: W.M.Wami@sms.ed.ac.uk

References

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Adesola, H., Uduak, N., Olajumoke, M., Roseangela, N., Chiaka, A., Sunday, A., Oyetunde, S., Ayodele, J. and Alex, O. (2012). Urine turbidity and microhaematuria as rapid assessment indicators for Schistosoma haematobium infection among school children in endemic areas. American Journal of Infectious Diseases 8, 6064.
Bergquist, R., Johansen, M. V. and Utzinger, J. (2009). Diagnostic dilemmas in helminthology: what tools to use and when? Trends in Parasitology 25, 151156.
Clements, A. C. a., Barnett, A. G., Nyandindi, U., Lwambo, N. J. S., Kihamia, C. M. and Blair, L. (2008). Age and gender effects in self-reported urinary schistosomiasis in Tanzania. Tropical Medicine and International Health 13, 713721.
Coulibaly, J. T., N'Gbesso, Y. K., Knopp, S., Keiser, J., N'Goran, E. K. and Utzinger, J. (2012). Efficacy and safety of praziquantel in preschool-aged children in an area co-endemic for Schistosoma mansoni and S. haematobium. PLoS Neglected Tropical Diseases 6, e1917.
Coulibaly, J. T., N'Goran, E. K., Utzinger, J., Doenhoff, M. J. and Dawson, E. M. (2013). A new rapid diagnostic test for detection of anti-Schistosoma mansoni and anti-Schistosoma haematobium antibodies. Parasites and Vectors 6, 29.
Dawson, E. M., Sousa-Figueiredo, J. C., Kabatereine, N. B., Doenhoff, M. J. and Stothard, J. R. (2013). Intestinal schistosomiasis in preschool-aged children of Lake Albert, Uganda: diagnostic accuracy of a rapid test for detection of anti-schistosome antibodies. Transactions of the Royal Society of Tropical Medicine and Hygiene 107, 639647.
de Noya, A. B., Ruiz, R., Losada, S., Colmenares, C., Contreras, R., Cesari, I. M. and Noya, O. (2007). Detection of schistosomiasis cases in low-transmission areas based on coprologic and serologic criteria: the Venezuelan experience. Acta Tropica 103, 4149.
Doenhoff, M. J., Chiodini, P. L. and Hamilton, J. V. (2004). Specific and sensitive diagnosis of schistosome infection: can it be done without antibodies? Trends in Parasitology 20, 14711492.
Doenhoff, M. J., Cioli, D. and Utzinger, J. (2008). Praziquantel: mechanisms of action, resistance and new derivatives for schistosomiasis. Current Opinion in Infectious Diseases 21, 659667.
Ekpo, U. F., Oluwole, A. S., Abe, E. M., Etta, H. E., Olamiju, F. and Mafiana, C. F. (2012). Schistosomiasis in infants and pre-school-aged children in sub-Saharan Africa: implication for control. Parasitology 10, 17.
Engels, D., Sinzinkayo, E., de Vlas, S. J. and Gryseels, B. (1997). Intraspecimen fecal egg count variation in Schistosoma mansoni infection. American Journal of Tropical Medicine and Hygiene 57, 571577.
French, M. D., Rollinson, D., Basanez, M. G. and Gryseels, B. (2007). School-based control of urinary schistosomiasis on Zanzibar, Tanzania: monitoring micro-haematuria with reagent strips as a rapid urological assessment. Journal of Pediatric Urology 3, 364368.
Garba, A., Barkiré, N., Djibo, A., Lamine, M. S., Sofo, B., Gouvras, A. N., Bosqué-Oliva, E., Webster, J. P., Stothard, J. R., Utzinger, J. and Fenwick, A. (2010). Schistosomiasis in infants and preschool-aged children: infection in a single Schistosoma haematobium and a mixed S. haematobiumS. mansoni foci of Niger. Acta Tropica 115, 212219.
Gryseels, B., Polman, K., Clerinx, J. and Kestens, L. (2006). Human schistosomiasis. Lancet 368, 11061118.
Hotez, P. J., Bundy, D. A. P., Beegle, K., Brooker, S., Drake, L., de Silva, N., Montresor, A., Engels, D., Jukes, M., Chitsulo, L., Chow, J., Laxminarayan, R., Michaud, C., Bethony, J., Correa-Oliveira, R., Shuhua, X., Fenwick, A. and Savioli, L. (2006). Infections: soil-transmitted helminth infections and schistosomiasis. In Disease Control Priorities in Developing Countries (ed. Jamison, D. T., Breman, J. G., Measham, A. R., Alleyne, G., Claeson, M., Evans, D. B., Jha, P., Mills, A. and Musgrove, P.), pp. 467482. Oxford University Press, New York, NY, USA.
Imai, N., Rujeni, N., Nausch, N., Bourke, C. D., Appleby, L. J., Cowan, G., Gwisai, R., Midzi, N., Cavanagh, D., Mduluza, T., Taylor, D. and Mutapi, F. (2011). Exposure, infection, systematic cytokine levels and antibody responses in young children concurrently exposed to schistosomiasis and malaria. Parasitology 138, 15191533.
Kabatereine, N. B., Brooker, S., Koukounari, A., Kazibwe, F., Tukahebwa, E. M., Fleming, F. M., Zhang, Y., Webster, J. P., Stothard, J. R. and Fenwick, A. (2007). Impact of a national helminth control programme on infection and morbidity in Ugandan schoolchildren. Bulletin of the World Health Organization 85, 9199.
Kahama, I., Nibbeling, H., van Zeyl, R. J., Vennervald, B. J., Ouma, J. H. and Deelder, M. (1998). Detection and quantification of soluble egg antigen in urine of Schistosoma haematobium-infected children from Kenya. American Journal of Tropical Medicine and Hygiene 59, 769774.
Kanamura, H. Y., Silva, R. M., Chiodelli, S. G., Glasser, C. M. and Dias, L. C. (2002). IgM-immunofluorescence test as a diagnostic tool for epidemiologic studies of schistosomiasis in low endemic areas. Memórias do Instituto Oswaldo Cruz 97, 485489.
Katz, N., Chaves, A. and Pellegrino, J. (1972). A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni. Revista do Instituto de Medicina Tropical de São Paulo 14, 397400.
King, C. H. and Bertsch, D. (2013). Meta-analysis of urine heme dipstick diagnosis of Schistosoma haematobium infection, including low-prevalence and previously treated populations. PLoS Neglected Tropical Diseases 7, e2431.
Kinkel, H. F., Dittrich, S., Baumer, B. and Weitzel, T. (2012). Evaluation of eight serological tests for diagnosis of imported schistosomiasis. Clinical Vaccine Immunology 19, 948953.
Lengeler, C., Utzinger, J. and Tanner, M. (2002). Questionnaires for rapid screening of schistosomiasis in sub-Saharan Africa. Bulletin of the World Health Organization 80, 235242.
Mathei, C., Shkedy, Z., Denis, B., Kabali, C., Aerts, M., Molenberghs, G., Van Damme, P. and Buntinx, F. (2006). Evidence for a substantial role of sharing of injecting paraphernalia other than syringes/needles to the spread of hepatitis C among injecting drug users. Journal of Viral Hepatitis 13, 560570.
Meyers, K. E. (2004). Evaluation of hematuria in children. Urologic Clinics of North America 31, 559573, x.
Mott, K., Baltes, R., Bambagha, J. and Baldassini, B. (1982). Field studies of a reusable polyamide filter for detection of Schistosoma haematobium eggs by urine filtration. Tropenmedizin und Parasitologie 33, 227228.
Muller, I., Coulibaly, J. T., Furst, T., Knopp, S., Hattendorf, J., Krauth, S. J., Stete, K., Righetti, A. A., Glinz, D., Yao, A. K., Puhse, U., N'Goran, E. K. and Utzinger, J. (2011). Effect of schistosomiasis and soil-transmitted helminth infections on physical fitness of school children in Cote d'Ivoire. PLoS Neglected Tropical Diseases 5, e1239.
Mutapi, F. (2011). Improving diagnosis of urogenital schistosome infection. Expert Review of Anti-infective Therapy 9, 863865.
Mutapi, F., Ndhlovu, P. D., Hagan, P. and Woolhouse, M. E. (1997). A comparison of humoral responses to Schistosoma haematobium in areas with low and high levels of infection. Parasite Immunology 19, 255263.
Mutapi, F., Hagan, P., Woolhouse, M. E. J., Mduluza, T. and Ndhlovu, P. D. (2003). Chemotherapy-induced, age-related changes in antischistosome antibody responses. Parasite Immunology 25, 8797.
Mutapi, F., Rujeni, N., Bourke, C., Mitchell, K., Appleby, L., Nausch, N., Midzi, N. and Mduluza, T. (2011). Schistosoma haematobium treatment in 1–5 year old children: safety and efficacy of the antihelminthic drug praziquantel. PLoS Neglected Tropical Diseases 5, e1143.
Nausch, N., Bourke, C. D., Appleby, L. J., Rujeni, N., Lantz, O., Trottein, F., Midzi, N., Mduluza, T. and Mutapi, F. (2012). Proportions of CD4+ memory T cells are altered in individuals chronically infected with Schistosoma haematobium. Scientific Reports 2, 472.
Ndhlovu, P., Cadman, H., Vennervald, B. J., Christensen, N. O., Chidimu, M. and Chandiwana, S. K. (1996). Age-related antibody profiles in Schistosoma haematobium infections in a rural community in Zimbabwe. Parasite Immunology 18, 181191.
Pasvol, G. and Hoffman, S. L. (2001). Schistosomiasis. Imperial College Press, London, UK.
Sady, H., Al-Mekhlafi, H. M., Mahdy, M. A., Lim, Y. A., Mahmud, R. and Surin, J. (2013). Prevalence and associated factors of schistosomiasis among children in Yemen: implications for an effective control programme. PLoS Neglected Tropical Diseases 7, e2377.
Smith, H., Doenhoff, M., Aitken, C., Bailey, W., Ji, M., Dawson, E., Gilis, H., Spence, G., Alexander, C. and van Gool, T. (2012). Comparison of Schistosoma mansoni soluble cercarial antigens and soluble egg antigens for serodiagnosing schistosome infections. PLoS Neglected Tropical Diseases 6, e1815.
Sorgho, H., Bahgat, M., Poda, J. N., Song, W., Kirsten, C., Doenhoff, M. J., Zongo, I., Ouedraogo, J. B. and Ruppel, A. (2005). Serodiagnosis of Schistosoma mansoni infections in an endemic area of Burkina Faso: performance of several immunological tests with different parasite antigens. Acta Tropica 93, 169180.
Sousa-Figueiredo, J. C., Basanez, M. G., Mgeni, A. F., Khamis, I. S., Rollinson, D. and Stothard, J. R. (2008). A parasitological survey, in rural Zanzibar, of pre-school children and their mothers for urinary schistosomiasis, soil-transmitted helminthiases and malaria, with observations on the prevalence of anaemia. Annals of Tropical Medicine and Parasitology 102, 679692.
Sousa-Figueiredo, J. C., Pleasant, J., Day, M., Betson, M., Rollinson, D., Montresor, A., Kazibwe, F., Kabatereine, N. B. and Stothard, J. R. (2010). Treatment of intestinal schistosomiasis in Ugandan preschool children: best diagnosis, treatment efficacy and side-effects, and an extended praziquantel dosing pole. International Health 2, 103113.
Stothard, J. R. and Gabrielli, A.-F. (2007). Schistosomiasis in African infants and preschool children: to treat or not to treat? Trends in Parasitology, 23, 8386.
Stothard, J. R., Sousa-Figueiredo, J. C., Betson, M., Adriko, M., Arinaitwe, M., Rowell, C., Besiyge, F. and Kabatereine, N. B. (2011 a). Schistosoma mansoni infections in young children: when are schistosome antigens in urine, eggs in stool and antibodies to eggs first detectable? PLoS Neglected Tropical Diseases 5, e938.
Stothard, J. R., Sousa-Figueiredo, J. C., Betson, M., Green, H. K., Seto, E. Y. W., Garba, A., Sacko, M., Mutapi, F., Vaz Nery, S., Amin, M. A., Mutumba-Nakalembe, M., Navaratnam, A., Fenwick, A., Kabatereine, N. B., Gabrielli, A. F. and Montresor, A. (2011 b). Closing the praziquantel treatment gap: new steps in epidemiological monitoring and control of schistosomiasis in African infants and preschool-aged children. Parasitology 138, 15931606.
Stothard, J. R., Sousa-Figueiredo, J. C., Betson, M., Bustinduy, A. and Reinhard-Rupp, J. (2013). Schistosomiasis in African infants and preschool children: let them now be treated! Trends in Parasitology 29, 197205.
van Dam, G. J., Wichers, J. H., Ferreira, T. M., Ghati, D., van Amerongen, A. and Deelder, A. M. (2004). Diagnosis of schistosomiasis by reagent strip test for detection of circulating cathodic antigen. Journal of Clinical Microbiology 42, 54585461.
van der Werf, M. J., de Vlas, S. J., Brooker, S., Looman, C. W., Nagelkerke, N. J., Habbema, J. D. and Engels, D. (2003). Quantification of clinical morbidity associated with schistosome infection in sub-Saharan Africa. Acta Tropica 86, 125139.
Vennervald, B. J., Kahama, I. and Reimert, C. M. (2000). Assessment of morbidity in Schistosoma haematobium infection: current methods and future tools. Acta Tropica 77, 8189.
Warrington, R., Watson, W., Kim, H. L. and Antonetti, F. R. (2011). An introduction to immunology and immunopathology. Allergy, Asthma and Clinical Immunology 7 (Suppl. 1), S1.
World Health Assembly (2001). Schistosomiasis and Soil-Transmitted Helminth Infections. WHA A54/VR/9. World Health Organization, Geneva, Switzerland.
World Health Organization (1998). Report of the WHO Informal Consultation on Schistosomiasis Control. WHO/CDS/CPC/SIP/99.2. World Health Organization, Geneva, Switzerland.
World Health Organization (2002). Prevention and Control of Schistosomiasis and Soil-Transmitted Helminthiasis. WHO Technical Report Series No. 912. World Health Organization, Geneva, Switzerland.
World Health Organization (2010). Working to Overcome the Global Impact of Neglected Tropical Diseases. WHO/HTM/NTD/2010.1. World Health Organization, Geneva, Switzerland.
World Health Organization (2011). Report of a Meeting to Review the Results of Studies on the Treatment of Schistosomiasis in Preschool-age Children. WHO/HTM/NTD/PCT/2011.7. World Health Organization, Geneva, Switzerland.
World Health Organization (2012). Research Priorities for Helminth Infections. WHO Technical Series No. 972. World Health Organization, Geneva, Switzerland.
World Health Organization (2013). Schistosomiasis: Progress Report 2001–2011 and Strategic Plan 2012–2020. WHO/HTM/NTD/PCT/2013.2. World Health Organization, Geneva, Switzerland.

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Comparing parasitological vs serological determination of Schistosoma haematobium infection prevalence in preschool and primary school-aged children: implications for control programmes

  • WELCOME M. WAMI (a1) (a2), NORMAN NAUSCH (a1) (a2), KATHARINA BAUER (a1) (a2), NICHOLAS MIDZI (a3), REGGIS GWISAI (a4), PETER SIMMONDS (a2), TAKAFIRA MDULUZA (a5), MARK WOOLHOUSE (a2) and FRANCISCA MUTAPI (a1) (a2)...

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