Skip to main content Accessibility help
×
Home

Characterization of two cDNAs encoding cysteine proteinases from the soybean cyst nematode Heterodera glycines

  • P. E. URWIN (a1), C. J. LILLEY (a1), M. J. McPHERSON (a1) and H. J. ATKINSON (a1)

Abstract

Two cDNAs encoding cysteine proteinases were isolated from a cDNA library constructed from feeding females of Heterodera glycines. The library was screened with a cysteine proteinase gene fragment originally amplified from cDNA of H. glycines. Database searches predict that 1 cDNA (hgcp-I) encodes a cathepsin L-like proteinase, while the second (hgcp-II) encodes a cathepsin S-like enzyme. Both predicted proteins contain a short secretion signal sequence, a long pro-peptide and a mature protein of 219 amino acids. Southern blot analysis suggests that the cathepsin S-like enzyme, HGCP-II, is encoded by a single-copy gene in contrast to the cathepsin L-like proteinase, HGCP-I which may have 2 homologues. The regions encoding the mature proteinases were cloned into an expression vector and recombinant protein produced in E. coli. HGCP-I was shown, after refolding, to cleave the synthetic peptide Z-Phe-Arg-AMC, and this activity could be inhibited by the engineered rice cystatin Oc-IΔD86. HGCP-II showed no activity against the synthetic substrates tested. The knowledge gained from these studies will improve our understanding of plant nematode proteinases and aid the development of a rational proteinase inhibitor-based approach to plant nematode resistance.

Copyright

Corresponding author

Centre for Plant Biochemistry and Biotechnology, The University of Leeds, Leeds LS2 9JT, UK. Tel: +0113 233 2863. Fax: +0113 233 3144. E-mail: pabhja@leeds.ac.uk

Keywords

Characterization of two cDNAs encoding cysteine proteinases from the soybean cyst nematode Heterodera glycines

  • P. E. URWIN (a1), C. J. LILLEY (a1), M. J. McPHERSON (a1) and H. J. ATKINSON (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed