Skip to main content Accessibility help
×
Home

Attempts to induce protective immunity in hamsters against infection by a liver fluke of man (Opisthorchis viverrini)

  • S. Sirisinha (a1), S. Tuti (a1), A. Tawatsin (a1), S. Vichasri (a2), E. S. Upatham (a2) and D. Bunnag (a3)...

Summary

The development of acquired resistance in opisthorchiasis was studied in hamsters experimentally infected with Opisthorchis viverrini. The induction of protective immunity was attempted by first exposing adult female golden Syrian hamsters to 1,2 or 3 doses of infective metacercariae obtained from naturally infected cyprinoid fishes and then reinfecting them with 80 metacercariae. In other experiments, animals that were infected with 50 metacercariae were treated with praziquantel prior to being rechallenged in order to eliminate the flukes that had developed from the first infection. The effect of long-term chronic infections was also studied. Faecal egg counts were determined at weekly intervals from 4–5 weeks onwards. The animals were killed 2–3 months after the last infection for worm recovery, and terminal faecal egg output/g faeces/worm was calculated. The data showed that prior infection of animals with O. viverrini did not induce significant protective immunity against reinfection by the same parasite. Lack of protection was also noted in animals reinfected several times with small doses of metacercariae. However, under certain circumstances, prior infection could result in a significant reduction in the faecal egg output due to subsequent infection.

Copyright

References

Hide All
Bhamarapravati, N., Thammavit, W. & Vajrasthira, S. (1978). Liver changes in hamsters infected with a liver fluke of man, Opisthorchis viverrini. American Journal of Tropical Medicine and Hygiene 27, 787–94.
Bunnag, D. & Harinasuta, T. (1981). Studies on the chemotherapy of human opisthorchiasis II. Minimum effective dose of praziquantel. Southeast Asian Journal of Tropical Medicine and Public Health 12, 413–17.
Flavell, D. J., Pattanapanyasat, K. & Flavell, S. U. (1980). Opisthorchis viverrini: partial success in adoptively transferring immunity with spleen cells and serum in the hamster. Journal of Helminthology 54, 191–7.
Flavell, D. J., Pattanapanyasat, K., Lucas, S. B. & Vongsangnak, V. (1980). Opisthorchis viverrini: liver changes in golden hamsters maintained on high and low protein diets. Acta tropica 37, 337–50.
Flavell, D. J. (1981 a). Liver-fluke infection as an aetiological factor in bile-duct carcinoma of man. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 814–23.
Flavell, D. J. (1981 b). The in vitro effects of serum on the adults, metacercariae, and eggs of Opisthorchis viverrini. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 825–31.
Harinasuta, C. (1969). Opisthorchiasis in Thailand: a review. In Proceedings of the 4th Southeast Asian Seminar on Parasitology and Tropical Medicine, Schistosomiasis and Other Snail-Transmitted Helminthiasis (ed. Harinasuta, C.), pp. 253–64. Bangkok, Thailand.
Janechaiwat, J., Tharavanij, S. & Vajrasthira, S. (1980). The immunological diagnosis of human opisthorchiasis and humoral immune response to opisthorchis infection in the hamster. Journal of Medical Association of Thailand 63, 439–47.
Katz, N., Chaves, A. & Pellegrino, J. (1972). A simple device for quantitative stool thick-smear technique in schistosomiasis. Revista do Instituto de Medicina Tropical de Sao Paulo 14, 397400.
Sokal, R. R. & Rohlf, F. J. (1969). Biometry: The Principles and Practice of Statistics in Biological Research, pp. 330–2. California: W. H. Freeman.
Sonakul, D., Koompirochana, C., Chinda, K. & Stitnimankarn, T. (1978). Hepatic carcinoma with opisthorchiasis. Southeast Asian Journal of Tropical Medicine and Public Health 9, 215–19.
Sun, T. (1969). The in vitro action of antisera on the adults of Clonorchis sinensis. Transactions of the Royal Society of Tropical Medicine and Hygiene 63, 582–90.
Sun, T. & Gibson, J. B. (1969). Antigens of Clonorchis sinensis in experimental and human infections: an analysis of gel-diffusion technique. American Journal of Tropical Medicine and Hygiene 18, 241–52.
Tuti, S., Vichasri, S. & Sirisinha, S. (1982). In vitro studies on Opisthorchis viverrini: Maintenance of adult flukes in culture medium. Journal of Parasitology (in the Press).
Vichasri, S. (1981). Some biological studies between the liver fluke Opisthorchis viverrini and its host. M.Sc. thesis, Mahidol University, Bangkok, Thailand.
Viranuvatti, V. & Stitnimankarn, T. (1972). Liver fluke infection and infestation in Southeast Asia. Progress in Liver Diseases 4, 537–47.
Wykoff, D. E., Harinasuta, C., Juttijudata, P. & Winn, M. M. (1965). Opisthorchis viverrini in Thailand: The life cycle and comparison with O.felineus. Journal ofParasitology 51, 507–14.
Wykoff, D. E. & Ariyaprakai, K. (1966). Opisthorchis viverrini in Thailand: Egg production in man and laboratory animals. Journal of Parasitology 52, 631.

Attempts to induce protective immunity in hamsters against infection by a liver fluke of man (Opisthorchis viverrini)

  • S. Sirisinha (a1), S. Tuti (a1), A. Tawatsin (a1), S. Vichasri (a2), E. S. Upatham (a2) and D. Bunnag (a3)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed