Skip to main content Accessibility help
×
Home

ATP diphosphohydrolase from Schistosoma mansoni egg: characterization and immunocytochemical localization of a new antigen

  • P. FARIA-PINTO (a1) (a2), M. N. L. MEIRELLES (a2), H. L. LENZI (a3), E. M. MOTA (a3), M. L. O. PENIDO (a1), P. M. Z. COELHO (a4) and E. G. VASCONCELOS (a1)...

Abstract

The fact that the Schistosoma mansoni egg has two ATP diphosphohydrolase (EC 3.6.1.5) isoforms with different net charges and an identical molecular weight of 63000, identified by non-denaturing polyacrylamide gel electrophoresis and immunological cross-reactivity with potato apyrase antibodies, is shown. In soluble egg antigen (SEA), only the isoform with the lower net negative charge was detected and seemed to be the predominant species in this preparation. By confocal fluorescence microscopy, using anti-potato apyrase antibodies, the S. mansoni egg ATP diphosphohydrolase was detected on the external surface of miracidium and in von Lichtenberg's envelope. Intense fluorescence was also seen in the outer side of the egg-shell, entrapped by the surface microspines, suggesting that a soluble isoform is secreted. ATP diphosphohydrolase antigenicity was tested using the vegetable protein as antigen. The purified potato apyrase was recognized in Western blots by antibodies present in sera from experimentally S. mansoni-infected mice. In addition, high levels of IgG anti-ATP diphosphohydrolase antibodies were detected by ELISA in the same sera. This work represents the first demonstration of antigenic properties of S. mansoni ATP diphosphohydrolase and immunological cross-reactivity between potato apyrase and sera from infected individuals.

Copyright

Corresponding author

Rua Barão de Cataguases 438/403, Sta Helena, Juiz de Fora, MG, 36015-370, Brasil. Tel: +55 32 3229 3217. Fax: +55 32 3229 3216. E-mail: eveline@icb.ufjf.br or evascon@powerline.com.br

References

Hide All

REFERENCES

ASHTON, P. D., HARROP, R., SHAH, B. & WILSON, R. A. ( 2001). The schistosome egg: development and secretions. Parasitology 122, 329338.
BOROS, D. L. & WARREN, K. S. ( 1970). Delayed hypersensitivity-type granuloma formation and dermal reaction induced and elicited by a soluble factor isolated from Schistosoma mansoni eggs. Journal of Experimental Medicine 132, 488507.
CESARI, I. M., BALLEN, D. E., PERRONE, T., ORIOL, O., HOEBEKE, J. & BOUT, D. ( 2000). Enzyme activities in Schistosoma mansoni soluble egg antigen. Journal of Parasitology 86, 11371140.
COIMBRA, E. S., GONÇALVES DA COSTA, S. C., CORTE-REAL, S., FREITAS, F. G. R., DURÃO, A., SOUZA, C. S. F., SILVA SANTOS, M. I. & VASCONCELOS, E. G. ( 2002). Characterization and cytochemical localization of an ATP diphosphohydrolase from Leishmania amazonensis promastigotes. Parasitology 124, 137143.
DeMARCO, R., KOWALTOWSKI, A. T., MORTARA, R. A. & VERJOVSKI-ALMEIDA, S. ( 2003). Molecular and characterization of Schistosoma mansoni ATP diphosphohydrolase. Biochemical and Biophysical Research Communications 307, 831838.
FILIPPINI, A., TAFFS, R. E., AGUI, T. & SITKOVSKY, M. V. ( 1990). Ecto-ATPase activity in cytolytic T-lymphocytes. Journal of Biological Chemistry 265, 334340.
GENDRON, F. P., BENREZZAK, O., KRUGH, B. W., KONG, Q., WEISMAN, G. A. & BEAUDOIN, A. R. ( 2002). Purine signaling and potential new therapeutic approach: possible outcomes of NTPDase inhibition. Current Drug Targets 3, 229245.
HANDA, M. & GUIDOTTI, G. ( 1996). Purification and cloning of a soluble ATP diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum). Biochemical and Biophysical Research Communications 218, 916923.
HARLOW, E. & LANE, D. ( 1988). Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
KETLLUN, A. M., URRA, R., LEYTON, M., VALENZUELA, M. A., MANCILLA, M. & TRAVERSO-CORI, A. ( 1992). Purification and characterization of two isoapyrases from Solanum tuberosum var. Ultimus. Phytochemistry 31, 36913696.
LAEMMLI, U. K. ( 1970). Cleavage of structural proteins during the assembly of the head of bacteriophages T4. Nature, London 227, 680685.
LOWRY, O. H., ROSEBROUGH, N. J., FARR, A. L. & RANDALL, R. J. ( 1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265275.
SMITHERS, S. R. & TERRY, R. J. ( 1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695700.
STADECKER, M. J., HERNANDEZ, H. J. & ASAHI, H. ( 2001). The identification and characterization of new immunogenic egg components: implications for evaluation and control of the immunopathogenic T cell response in schistosomiasis. Memórias do Instituto Oswaldo Cruz 96, 2933.
TAUSSKY, H. M. & SHORR, E. ( 1953). A microcolorimetric method for the determination of inorganic phosphorus. Journal of Biological Chemistry 202, 675685.
VARGAFTIG, B. B., CHIGNARD, M. & BENEVISTE, J. ( 1981). Present concepts on the mechanism of platelet aggregation. Biochemical Pharmacology 30, 263271.
VASCONCELOS, E. G., FERREIRA, S. T., CARVALHO, T. M. U., SOUZA, W., KETTLUN, A. M., MANCILLA, M., VALENZUELA, M. A. & VERJOVSKI-ALMEIDA, S. ( 1996). Partial purification and immunohistochemical localization of ATP diphosphohydrolase from Schistosoma mansoni. Journal of Biological Chemistry 271, 2213922145.

Keywords

ATP diphosphohydrolase from Schistosoma mansoni egg: characterization and immunocytochemical localization of a new antigen

  • P. FARIA-PINTO (a1) (a2), M. N. L. MEIRELLES (a2), H. L. LENZI (a3), E. M. MOTA (a3), M. L. O. PENIDO (a1), P. M. Z. COELHO (a4) and E. G. VASCONCELOS (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed