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Antiparasitic efficacy of Gracillin and Zingibernsis newsaponin from Costus speciosus (Koen ex. Retz) Sm. against Ichthyophthirius multifiliis

  • WEI ZHENG (a1), CHUN-MEI YAN (a1), YA-BIN ZHANG (a1), ZE-HONG LI (a2), ZHONGQIANG LI (a1), XIU-YING LI (a1), ZHANG-WEI WANG (a1), XIULAN WANG (a1), WEI-QIANG CHEN (a1) and XIN-HAI YU (a2)...

Summary

The present study aims to evaluate the antiparasitic activity of active components from Costus speciosus against Ichthyophthirius multifiliis. Bioassay-guided fractionation was employed to identify active compounds from C. speciosus yielding 2 bioactive compounds: Gracillin and Zingibernsis newsaponin. In-vitro assays revealed that Gracillin and Zingibernsis newsaponin could be 100% effective against I. multifiliis at concentrations of 0·8 and 4·5 mg L−1, with median effective concentration (EC50) values of 0·53 and 3·2 mg L−1, respectively. All protomonts and encysted tomonts were killed when the concentrations of Gracillin and Zingibernsis newsaponin were 1·0 and 5·0 mg L−1. In-vivo experiments demonstrated that fish treated with Gracillin and Zingibernsis newsaponin at concentrations of 1·0 and 5·0 mg L−1 carried significantly fewer parasites than the control (P<0·05). Mortality of fish did not occur in the treatment group (Zingibernsis newsaponin at 5·0 mg L−1) during the trial, although 100% of untreated fish died. Acute toxicities (LD50) of Gracillin and Zingibernsis newsaponin for grass carp were 1·64 and 20·7 mg L−1, respectively. These results provided evidence that the 2 compounds can be selected as lead compounds for the development of new drugs against I. multifiliis.

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Corresponding author

* Corresponding author: Jilin Province Fisheries Research Institute, 1195 Kunshan Road, Changchun, Jilin, China. E-mail: yaojiamm131@163.com

References

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Battacharya, S. K., Parik, A. K., Debnath, P. K., Pandey, V. B. and Neogy, N. C. (1972). Anticholiesterase activity of Costus speciosus alkaloids Indian . Journal of Pharmacology 4, 178179.
Callinan, R. B. and Rowland, S. J. (1995). Diseases of silver perch. In Silver Perch Culture (ed. Rowland, S. J. and Bryant, C.), pp. 6775. Austasia Aquaculture, Sandy Bay, Australia.
Chen, C. X. and Yin, H. X. (1995). Steroidal saponins from Costus speciosus . Natural Product Research and Development 7, 1823.
Cheung, J. Y., Ong, R. C., Suen, Y. K., Ooi, V., Wong, H. N. and Mak, T. C. (2005). Polyphyllin D is a potent apoptosis inducer in drug-resistant HepG2 cells. Cancer Letter 217, 203211.
Chu, C., Zhang, Q. Z. and Luo, F. (2010). Effect of twenty Chinese herbal medicines on killing trophonts, cysts and theronts of Ichthyophthirius multifiliis in vitro. Freshwater Fishery 40, 5560 (Chinese with English abstract).
Clark, T. G. and Dickerson, H. W. (1997). Antibody-mediated effects on parasite behavior: evidence of a novel mechanism of immunity against a parasitic protist. Parasitology Today 13, 477480.
Clayton, G. M. and Price, D. J. (1988). Ichthyophthirius multifiliis: standardization of the infection-response model in Ameca splendens (Miller & Fitzsimons). Journal of Fish Diseases 11, 371377.
Eliza, J., Daisy, P., Ignacimuthu, S. and Duraipandiyan, V. (2009). Antidiabetic and antilipidemic effect of eremanthin from Costus speciosus (Koen.) Sm. in STZ-induced diabetic rats. Chemico-Biological Interactions 182, 6772.
Ghada, A. H., Lahnsteiner, F. and Mansour, N. (2014). Possibilities to control Ichthyophthirius multifiliis infestation with medicated feed in rainbow trout (Oncorhynchus mykiss) and chub (Leuciscus cephalus). Parasitology Research 113, 11191126.
Jain, D. C. (1987). Antifeedant active saponins from Balanites roxburghii stem bark. Phytochemistry 26, 22232225.
Kang, L. P., Ma, B. P. and Wang, Y. (2005). Study on separation and identification of steroidal saponins of Dioscorea nipponica Makinlo. Chinese Pharmaceutical Journal 40, 15391541.
Lahnsteiner, F. and Weismann, T. (2007). Treatment of ichthyophthiriasis in rainbow trout and common carp with common and alternative therapeutics. Journal of Aquatic Animal Health 19, 186194.
Lee, M. S., Yuet-Wa, J. C., Kong, S. K., Yu, B., Eng-Choon, V. O. and Nai-Ching, H. W. (2005). Effects of polyphyllin D, a steroidal saponin in Paris polyphylla, in growth inhibition of human breast cancer cells and in xenograft. Cancer Biology Therapy 4, 12481254.
Li, Z. H., Wan, J. Y., Wang, G. Q., Zhao, F. G., Wen, J. H. and Wen, J. H. (2013). Identification of compounds from Paris polyphylla (ChongLou) active against Dactylogyrus intermedius . Parasitology 140, 952954.
Ling, F., Wang, J. G., Liu, Q. F., Li, M., Ye, L. T. and Gong, X. L. (2010). Prevention of Ichthyophthirius multifiliis infestation in goldfish (Carassius auratus) by potassium ferrate (VI) treatment. Veterinary Parasitology 168, 212216.
Ling, F., Wang, J. G., Lu, C., Wang, G. X., Lui, Y. H. and Gong, X. N. (2012). Effects of aqueous extract of Capsicum frutescens (Solanaceae) against the fish ectoparasite Ichthyophthirius multifiliis . Parasitol Res 111, 841848.
Newman, D. J., Cragg, G. M. and Snader, K. M. (2003). Natural products as sources of new drugs over the period 1981–2002. Journal of Natural Products 66, 10221037.
Shan, X. F., Meng, Q. F., Kang, Y. H., Bian, Y., Gao, Y. H., Wang, W. L. and Qian, A. D. (2014). Isolation of active compounds from methanol extracts of Toddalia asiatica against Ichthyophthirius multifiliis in goldfish (Carassius auratus). Veterinary Parasitology 199, 34.
Shinn, A. P., Picón-Camacho, S. M., Bron, J. E., Conway, D., Yoon, G. H., Guo, F. C. and Taylor, N. G. H. (2012). The anti-protozoal activity of bronopol onthe key life-stages of Ichthyophthirius multifiliis Fouquet, 1876 (Cilio-phora). Veterinary Parasitology 186, 229236.
Sparg, S. G., Light, M. E. and Staden, J. V. (2004). Biological activities and distribution of plant saponins. Journal of Ethnopharmacology 94, 219243.
Traxler, G. S., Richard, J. and McDonald, T. E. (1998). Ichthyophthirius multifiliis (Ich) epizootics in spawning sockeye salmon in British Columbia, Canada. Journal of Aquatic Animal Health 10, 143151.
Wang, Y., Cheung, Y. H. and Yang, Z. (2006). Proteomic approach to study the cytotoxicity of dioscin (saponin). Proteomics 6, 24222432.
Wohllebe, S., Richter, P. and Häder, D. P. (2012). Chlorophyllin for the control of Ichthyophthirius multifiliis (Fouquet). Parasitology Research 111, 729733.
Yao, J. Y., Shen, J. Y., Li, X. L., Xu, Y., Hao, G. J., Pan, X. Y., Wang, G. X. and Yin, W. L. (2010). Effect of sanguinarine from the leaves of Macleaya cordata against Ichthyophthirius multifiliis in grass carp (Ctenopharyngodon idella). Parasitology Research 107, 10351042.
Yao, J. Y., Li, X. L., Shen, J. Y., Pan, X. Y., Hao, G. J., Xu, Y., Ying, W. L., Ru, H. S. and Liu, X. L. (2011). Isolation of bioactive components from Chelidonium majus L. with activity against Trichodina sp. Aquaculture 318, 235238.
Yi, Y. L., Lu, C., Hu, X. G., Ling, F. and Wang, G. X. (2012). Antiprotozoal activity of medicinal plants against Ichthyophthirius multifiliis in goldfish (Carassius auratus). Parasitology Research 111, 17711778.
Zhang, Q. Z., Xu, D. H. and Klesius, P. H. (2013). Evaluation of an antiparasitic compound extracted from Gallachinensis against fish parasite Ichthyophthirius multifiliis . Veterinary Parasitology 198, 4553.

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