Hostname: page-component-76fb5796d-qxdb6 Total loading time: 0 Render date: 2024-04-25T10:22:11.263Z Has data issue: false hasContentIssue false
  • English
  • Français

Antigenic analysis of a variant-specific component of Trypanosoma brucei brucei: localization on the surface coat with labelled specific antibodies

Published online by Cambridge University Press:  06 April 2009

J. Fruit ,
D. Afchain ,
A. Petitprez ,
N. Van Meirvenne ,
D. Le Ray ,
D. Bout  and
A. Capron
J. Fruit
Affiliation:
Services d'immunologie et de Biologie Parasitaire (U.E.R. de Médecine) et de Parasitologie (U.E.R. de Pharmacie), 59000 Lille, France
D. Afchain
Affiliation:
Services d'immunologie et de Biologie Parasitaire (U.E.R. de Médecine) et de Parasitologie (U.E.R. de Pharmacie), 59000 Lille, France
A. Petitprez
Affiliation:
Laboratoire de Microscopie Electronique, Institut Pasteur, 59000 Lille
N. Van Meirvenne
Affiliation:
Laboratoires de Protozoologie et de Sérologie, Institut de Médecine Tropicale, 2000 Antwerpen, Belgium
D. Le Ray
Affiliation:
Laboratoires de Protozoologie et de Sérologie, Institut de Médecine Tropicale, 2000 Antwerpen, Belgium
D. Bout
Affiliation:
Services d'immunologie et de Biologie Parasitaire (U.E.R. de Médecine) et de Parasitologie (U.E.R. de Pharmacie), 59000 Lille, France
A. Capron
Affiliation:
Services d'immunologie et de Biologie Parasitaire (U.E.R. de Médecine) et de Parasitologie (U.E.R. de Pharmacie), 59000 Lille, France
Get access Rights & Permissions [Opens in a new window]

Extract

The nature of antigens present on the surface coat of Trypanosoma brucei brucei (serotype AnTat–1) trypanosomes has been investigated by light and electron microscopy using indirect immunofluorescence and immunoenzymatic labelling techniques. The use of a monospecific anticomponent V serum has shown that the variant-specific antigen, component V, is the major constituent of the surface coat. Antigens of heterologous serotype, of culture form trypanosomes, of host serum proteins or host red blood cells were not demonstrated on the surface coat.

Type
Research Article
Information
Parasitology , Volume 74 , Issue 2 , April 1977 , pp. 185 - 190
Copyright
Copyright © Cambridge University Press 1977

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Afchain, D., Fruit, J., Petitprez, A., Van MeirvenneN., Le N., Le, Ray, D., Bout, D. & Capron, A. (1976). Antigenic analysis of a variant specific component of Trypanosoma brucei brucei. I-Immunochemical purification by affinity chromatography. Pathologie Biologie 24, 615–17.Google ScholarPubMed
Avrameas, S. (1969). Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry 6, 43–52.CrossRefGoogle ScholarPubMed
Cross, G. A. M. (1975). Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei. Parasitology 71, 393–417.CrossRefGoogle ScholarPubMed
Cunningham, M. P. & Vickerman, K. (1962). Antigenic analysis in the Trypanosoma brucei group, using the agglutination reaction. Transactions of the Royal Society of Tropical Medicine and Hygiene 56, 48–59.CrossRefGoogle ScholarPubMed
Gray, A. R. (1962). The influence of antibody on serological variation in Trypanosoma brucei. Annals of Tropical Medicine and Parasitology 56, 4–13.CrossRefGoogle ScholarPubMed
Jadin, J. & Le, Ray D. (1969). Acquisitions récentes dans les techniques de culture des Trypanomoses africains. Annales de la Société belge de Médecine tropicale 49, 331–40.Google Scholar
Ketteridge, D. (1972). Trypanosoma vivax: surface interrelationships between host and parasite. Transactions of the Royal Society of Tropical Medicine and Hygiene 66, 324.CrossRefGoogle ScholarPubMed
Lanham, S. M. (1968). Separation of trypanosomes from the blood of infected rats and mice by anion-exchangers. Nature, London 218, 1273–4.CrossRefGoogle ScholarPubMed
Le Ray, D. (1975). Structures antigéniques de Trypanosoma brucei (Protozoa, Kinetoplastida). Analyse immunoélectrophorétique et étude comparative. Annals de la Société belge de Médecine tropicale 55, 129–311.Google ScholarPubMed
Levaditi, D. & Mutermilch, S. (1909). Le mécanisme de la création des variétés de trypanosomes résistant aux anticorps. Comptes Rendus des séances de la Société de Biologie 67, 49–51.Google Scholar
Lourie, E. M. & O'Connor, R. J. (1937). A study of Trypanosoma rhodesiense relapse strains in vitro. Annals of Tropical Medicine and Parasitology 31, 319–40.CrossRefGoogle Scholar
Takayanagi, T., Enriquez, G. L., Kambara, H. & Ohno, Y. (1973). An immuno-electron microscopy of the soluble antigen of Trypanosoma gambiense. Southeast Asian Journal of Tropical Medicine and Public Health 4, 165–7.Google ScholarPubMed
Vaitukaitis, J., Robbins, J. B., Nieschlag, E. & Ross, T. G. (1971). A method for producing specific antisera with small doses of immunogen. Journal of Clinical Endocrinology and Metabolism 33, 988–91.CrossRefGoogle ScholarPubMed
Van Meirvenne, N., Janssens, P. G. & Magnus, E. (1975). Antigenic variation in syringepassaged populations of Trypanosoma (Trypanozoon) brucei. I- Rationalization of the experimental approach. Annales de la Société belge de Médecine tropicale 55, 1–23.Google Scholar
Vickerman, K. & Luckins, A. G. (1969). Localization of variable antigens in the surface coat of Trypanosoma brucei using ferritin conjugated antibody. Nature, London 224, 1125–6.CrossRefGoogle ScholarPubMed