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Anticoccidial activity of herbal complex in broiler chickens challenged with Eimeria tenella

  • MUHAMMAD A. ZAMAN (a1), ZAFAR IQBAL (a1), RAO Z. ABBAS (a1) (a2) and MUHAMMAD N. KHAN (a1)

Summary

The objective of the present study was to evaluate the anticoccidial effect of different concentrations of the herbal complex of 4 plants (leaves of Azadirachta indica and Nicotiana tabacum, flowers of Calotropis procera and seeds of Trachyspermum ammi) in broiler chickens in comparison with amprolium anticoccidial. Three concentrations (2 g, 4 g and 6 g) of herbal complex were given to the experimental groups once a day and amprolium (at the dose rate of 125 ppm) was given orally in drinking water from the 14th to the 21st days of age. One group was kept as infected, non-medicated control and one as non-infected, non-medicated control. All groups were inoculated orally with 75 000 sporulated oocysts on the 14th day of age except the non-infected, non-medicated control. Among herbal complex medicated groups, the maximum anticoccidial effect was seen in the group medicated with 6 g herbal complex followed by 4 g and 2 g herbal complex medicated groups. Treatment with 6 g of the herbal complex significantly reduced the negative performance and pathogenic effects associated with Eimeria tenella challenge at a level that was comparable with amprolium when using a largely susceptible recent field isolate. In summary, concentration-dependent anticoccidial activity of the studied herbal complex suggests its use as an alternative anticoccidial agent to chemotherapeutic drugs for Eimeria tenella control.

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Corresponding author

*Corresponding author: Department of Parasitology, University of Agriculture, Faisalabad-38040-Pakistan. Tel: +92 41 9201106. E-mail: raouaf@hotmail.com

References

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Abbas, R. Z., Iqbal, Z., Akhtar, M. S., Khan, M. N., Jabbar, A. and Sandhu, Z. D. (2006). Anticoccidial Screening of Azadirachta indica (Neem) in broilers. Pharmacologyonline 3, 365371.
Abbas, R. Z., Iqbal, Z., Blake, D., Khan, M. N. and Saleemi, M. K. (2011 a). Anticoccidial drug resistance in fowl coccidia: the state of play revisited. World’s Poultry Science Journal 67, 337350.
Abbas, R. Z., Iqbal, Z., Khan, M. N., Zafar, M. A. and Zia, M. A. (2010). Anticoccidial activity of Curcuma longa L. in Broiler Chickens. Brazilian Archives of Biology and Technology 53, 6367.
Abbas, R. Z., Iqbal, Z., Sindhu, Z. D., Khan, M. N. and Arshad, M. (2008). Identification of cross resistance and multiple resistance in Eimeria tenella field isolates to commonly used anticoccidials in Pakistan. Journal of Applied Poultry Research 17, 361368.
Abbas, R. Z., Manzoor, Z., Munawar, S. H., Iqbal, Z., Khan, M. N., Saleemi, M. K., Zia, M. A. and Yousaf, A. (2011 c). Anticoccidial activity of hydrochloric acid (HCl) against Eimeria tenella in broiler chickens. Pesquisa Veterinária Brasileira 31, 425429.
Abbas, R. Z., Munawar, S. H., Manzoor, Z., Iqbal, Z., Khan, M. N., Saleemi, M. K., Zia, M. A. and Yousaf, A. (2011 b). Anticoccidial effects of acetic acid on performance and pathogenic parameters in broiler chickens challenged with Eimeria tenella. Pesquisa Veterinária Brasileira 31, 99103.
Adjanohoun, E. J., Cusset, G., Waechter, P., Le, I., Keita, A., Le, B. M. and Lejoly, J. (1989). Banque de données de médecine traditionnelle et pharmacopée (PHARMEL). ACCT (Agence de Coopération Culturelle et technique). Paris, France.
Aeschbach, R., Loliger, J., Scott, B. C., Murica, A., Butler, J., Halliwell, B. and Aruoma, O. I. (1994). Antioxidant actions of thymol, carvacol, 6-gingerol, zingerone and hudroxytyroslo. Food Chemistry and Toxicology 32, 3136.
Ak´e Assi, Y. A. (1992). Contribution au recensement des esp`eces v´eg´etales utilis´ees traditionnellement sur le plan zootechnique et v´et´erinaire en Afrique de l'Ouest. Ph.D. Thesis, Universit´e Claude-Bernard Lyon I, France.
Al-Rajhy, D. H., Alahmed, A. M., Hussein, H. I. and Kheir, S. M. (2003). Acaricidal effects of cardiac glycosides, azadirachtin and neem oil against the camel tick, Hyalomma dromedarii (Acari: Ixodidae). Pest Managment Science 59, 12501254.
Amaral, J. A., Ekins, A., Richards, S. R. and Knowles, R. (1998). Effect of selected monoterpenes on methane oxidation, denitrification, and aerobic metabolism by bacteria in pure culture. Applied Environmental Microbiology 64, 520525.
Awais, M. M., Akhtar, M., Muhammad, F., Haq, A. U. and Anwar, M. I. (2011). Immunotherapeutic effects of some sugar cane (Saccharum officinarum L.) extracts against coccidiosis in industrial broiler chickens. Experimental Parasitology 128, 104110.
Ayaz, M., Akhtar, M., Hayat, C. S., Hafeez, M. A. and Haq, A. (2003). Prevalence of coccidiosis in broiler chickens in Faisalabad, Pakistan. Pakistan Veterinary Journal 23, 5152.
Barre, J. T., Bowden, B. F., Coll, J. C., Jesus, J., Fuente, V. E., Janairo, G. C. and Ragasa, C. Y. (1997). A bioactive triterpene from Lantana camara. Phytochemistry 45, 321324.
Biu, A. A., Yusuf, S. D. and Rabo, J. S. (2006). Use of neem (Azadirachta indica) aqueous extract as a treatment for poultry coccidiosis in Borno State, Nigeria. African Scientist 7, 147153.
Bowman, W. C. and Rand, M. J. (1980). Textbook of Pharmacology. Blackwell Scientific Publications, Oxford, UK.
Christaki, E., Florou-Paneri, P., Giannenea, I., Papzahariadou, M., Botsoglou, N. A. and Spais, A. S. B. (2004). Effect of a mixture of herbal extracts on broiler chickens infected with Eimeria tenella. Animal Research 53, 137144.
Cook, G. C. (1988). Small intestinal coccidiosis: An emergent clinical problem. Journal of Infection 16, 213219.
Costa, C. T. C., Bevilaqua, C. M. L., Camurça-Vasconcelos, A. L. F., Maciel, M. V., Morais, S. M., Castro, C. M. S., Braga, R. R. and Oliveira, L. M. B. (2008). In vitro ovicidal and larvicidal activity of Azadirachta indica extracts on Haemonchus contortus. Small Ruminant Research 74, 284287.
Crespy, V. and Williamson, G. (2004). A review of the health effects of green tea catechins in in vivo animal models. Journal of Nutrition 134, 3431S3440S.
Dalloul, R. A. and Lillehoj, H. S. (2006). Poultry coccidiosis: Recent advancements in control measures and vaccine development. Expert Review of Vaccines 5, 143163.
Dhar, D. N. and Singh, R. K. (1973). The chemistry of Calotropis procera. The Eastern Pharmacist 176, 99101.
Duffy, C. F., Mathis, G. F. and Power, R. F. (2005). Effects of Natustat™ supplementation on performance, feed efficiency and intestinal lesion scores in broiler chickens challenged with Eimeria acervulina, Eimeria maxima and Eimeria tenella. Veterinary Parasitology 130, 185190.
Finlay, R. C., Roberts, S. J. and Hayday, A. C. (1993). Dynamic response of murine gut intraepithelial T cells after infection by the coccidian parasite Eimeria. European Journal of Immunology 23, 25572564.
Fujiki, H. (2005). Green tea: health benefits as cancer preventive for humans. The Chemical Record 5, 119132.
Haslam, E. (1996). Natural polyphenols (vegetable tannins) as drugs: possible modes of action. Journal of Natural Products 59, 205215.
Hilbrich, P. (1978). Krankheiten des Geflugels unter besondered Berucksichigigung der Haltung und Futterung. Hermann Kuhn KG, Schwenningen am Neckar, Germany.
Hopp, K. H., Cunningham, L. V., Bromel, M. C., Schermeister, L. J. and Wahba Khalil, S. K. (1976). In vitro antitrypanosomal activity of certain alkaloids against Trypanosoma lewisi. Lloydia 39, 375377.
Iqbal, Z., Jabbar, A., Akhtar, M. S., Muhammad, G. and Lateef, M. (2005). Possible role of ethno- veterinary medicine in poverty reduction in Pakistan: Use of botanical anthelmintics as an example. Journal of Agriculture and Social Sciences 1, 187195.
Iqbal, Z., Lateef, M., Jabbar, A., Ghayur, M. N. and Gilani, A. H. (2006). In vitro and In vivo anthelmintic activity of Nicotiana tabacum L. Leaves against gastrointestinal nematodes of sheep. Phytotherapy Research 20, 4648.
Iqbal, Z., Lateef, M., Jabbar, A. and Gilani, A. H. (2010). In vivo anthelmintic activity of Azadirachta indica A. Juss seeds against gastrointestinal nematodes of sheep. Veterinary Parasitology 168, 342345.
Isogai, E., Isogai, H., Hirose, K., Hayashi, S. and Oguma, K. (2001). In vivo synergy between green tea extract and levofloxacin against enterohemorrhagic Escherichia coli O157 infection. Current Microbiology 42, 248251.
Johnson, J. and Reid, W. M. (1970). Anticoccidial drugs: lesion scoring techniques in battery and floor pen experiments with chickens. Experimental Parasitology 28, 3036.
Jones, G. A., McAllister, T. A., Muir, A. D. and Cheng, K. J. (1994). Effects of sainfoin (Onobrychis viciifolia scop.) condensed tannins on growth and proteolysis by four strains of ruminal bacteria. Applied Environmental Microbiology 60, 13741378.
Khajeh, M., Yamini, Y., Fatemeh, S. and Bahramifar, N. (2004). Comparison of essential oil composition of Carum copticum obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Food Chemistry 86, 587591.
Kilonzo, B. S., Ngomuo, A. J., Sabuni, C. A. and Mgode, G. F. (2001). Effects of Azadirachta indica (Neem) extract on livestock fleas in Morogoro District, Tanzania. Insect Science and its Application 21, 8992.
Lateef, M., Iqbal, Z., Akhtar, M. S., Jabbar, A., Khan, M. N. and Gilani, A. H. (2006). Preliminary screening of Trachyspermum ammi (L.) seed for anthelmintic activity in sheep. Tropical Animal Health and Production 38, 491496.
Laurent, F., Mancassola, R., Lacroix, S., Menezes, R. and Naciri, M. (2001). Analaysis of chicken mucosal immune response to Eimeria tenella and Eimeria maxima infection by quantitative reverse transcription-PCR. Infection and Immunity 69, 25272534.
MAFF. (1986). Parasitological Laboratory Techniques. Technical Bulletin no.18, Manual of Veterinary, Her Majestey's Stationary Office, Ministry of Agriculture, Fisheries and Food, London, UK.
Mason, T. L. and Wasserman, B. P. (1987). Inactivation of red beet betaglucan synthase by native and oxidized phenolic compounds. Phytochemistry 26, 21972202.
McAllister, T. A., Wang, Y., Hristov, A. N., Olson, M. E. and Cheeke, P. R. (1998). Applications of Yucca schidigera in livestock production. Proceedings of the 33rd Pacific Northwest Animal Nutrition Conference, Canada, pp. 109119.
Molan, A. L., Meagher, L. P., Spencer, P. A. and Sivakumaran, S. (2003). Effect of flavan-3-ols on in vitro egg hatching, larval development and viability of infective larvae of Trichostrongylus colubriformis. International Journal for Parasitology 33, 16911698.
Molan, A. L., Sivakumaran, S., Spencer, P. A. and Meagher, L. P. (2004). Green tea flavan-3-ols and oligomeric proanthocyanidins inhibit the motility of infective larvae of Teladorsagia circumcincta and Trichostrongylus colubriformis in vitro. Research in Veterinary Science 77, 239243.
Montes-Belmont, R. and Carvajal, M. (1998). Control of Aspergillus flavus in maize with plant essential oils and their components. Journal of Food Protection 61, 616619.
Mossa, J. S., Tariq, M., Mohsin, A., Ageel, A. M., Al-Yahya, M. A., Al-Said, M. S. and Rafatullah, S. (1991). Pharmacological studies on aerial parts of Calotropis procera. American Journal of Chinese Medicine 19, 223231.
Nagalakshmi, S., Shankaracharya, N. B., Naik, J. P. and Rao, L. J. M. (2000). Studies on chemical and technological aspects of ajowan (Trachyspermum ammi (L.) Syn. Carum copticum Hiern) seeds. Journal of Food Science and Technology 37, 277281.
National Research Council (1992) Neem: a Tree for Solving Global Problems. National Academy Press, Washington D.C.
Nogueira, V. A., Franca, T. N. and Peixoto, P. V. (2009). Ionophore poisoning in animals. Pesquisa Veterinária Brasileira 29, 191197.
Ogaard, B., Larsson, E., Glans, R., Henriksson, T. and Birkhed, D. (1997). Antimicrobial effect of a chlorhexidine-thymol varnish (cervitec) in orthodontic patients. A prospective, randomized clinical trial. Journal of Orofacial Orthopedics 58, 206213.
Omulokoli, E., Khan, B. and Chhabra, S. C. (1997). Antiplasmodial activity of four Kenyan medicinal plants. Journal of Ethnopharmacology 56, 133137.
Pandey, N., Chandrakar, A. K., Garg, M. L. and Patel, S. S. (2009). Calotropris procera-induced keratitis. Indian Journal of Ophthalmology 57, 5860.
Phillipson, J. D. and O'Neill, M. J. (1987). New leads to the treatment of protozoal infections based on natural product molecules. Acta Pharmaceutica Nordica 1, 131144.
Rosell, G., Quero, C., Coll, J. and Guerrero, A. (2008). Biorational insecticides in pest management. Journal of Pest Sciences 33, 103121.
Saima, M. Z. U., Jabbar, M. A., Mehmud, A., Abbas, M. M. and Mahmood, A. (2010). Effect of lysine supplementation in low protein diets on the performance of growing broilers. Pakistan Veterinary Journal 30, 1720.
Seiber, J. N., Nelson, C. J. and Lee, S. M. (1982). Cardenolides in the latex and leaves of seven Asclepias species and Calotropis procera. Phytochemistry 21, 23432348.
Sen, S., Sahu, N. P. and Mahato, S. B. (1992). Flavonol glycosides from Calotropis gigantea. Phytochemistry 31, 29192921.
Sharma, P. and Sharma, J. D. (1999). Evaluation of in vitro schizontocidal activity of plant parts of Calotropis procera – an ethnobotanical approach. Journal of Ethnopharmacology 68, 8395.
Shirley, M. W. (1986). New methods for the identification of species and strains of Eimeria. In Research in Avian Coccidiosis. (ed. McDougald, L. R., Long, P. L. and Joyner, L. P.), pp. 1335. University of Georgia, Athens, GA, USA.
Singh, B. and Rastogi, R. P. (1972). Structure of asclepin and some observations on the NMR spectra of Calotropis glycosides. Phytochemistry 11, 757762.
Stern, J. L., Hagerman, A. E., Steinberg, P. D. and Mason, P. K. (1996). Phlorotannin-protein interactions. Journal of Chemical Ecology 22, 18871899.
Sundaram, K. M. S. and Sloane, L. (1995). Effects of pure and formulated azadirachtin, a neem-based biopesticide, on the phytophagous spider mite, Tetranychus urticae Koch. Journal of Environmental Science and Health 30, 801814.
Tipu, M. A., Pasha, T. N. and Ali, Z. (2002). Comparative efficacy of salinomycin sodium and neem fruit (Azadirachta indica) as feed additive anticoccidials in broilers. International Journal of Poultry Sciences 1, 9193.
Toulah, F. H., Ismeel, H. A. and Khan, S. (2010). Effect of treatment with Neem (Azadirachta indica) compared with Baycox drug on the caecum of chicken experimentally infected with Eimeria tenella. Journal of the Egyptian Society of Parasitology 40, 93106.
Tsuchiya, H., Sato, M., Miyazaki, T., Fujiwara, S., Tanigaki, S., Ohyama, M., Tanaka, T. and Iinuma, M. (1996). Comparative study on the antibacterial activity of phytochemical flavanones against methicillin-resistant Staphylococcus aureus. Journal of Ethnopharmacology 50, 2734.
Wang, Y., McAllister, T. A., Newbold, C. J., Rode, L. M., Cheeke, P. R. and Cheng, K. J. (1998). Effects of Yucca schidigera extract on fermentation and degradation of steroidal saponins in the rumen simulation technique (RUSITEC). Animal Feed Science and Technolnology 74, 143153.
Weber, J. M., Ruzindana-Umunyana, A., Imbeault, L. and Sircar, S. (2003). Inhibition of adenovirus infection and adenain by green tea catechins. Antiviral Research 58, 167173.
Worku, M., Franco, R. and Miller, J. H. (2009). Evaluation of the Activity of Plant Extracts in Boer Goats. American Journal of Animal and Veterinary Sciences 4, 7279.
Ya, C., Gaffney, S. H., Lilley, T. H. and Haslam, E. (1988). Carbohydratepolyphenol complexation. In Chemistry and Significance of Condensed Tannins (ed. Hemingway, S. R. W. and Karchesy, J. J.), pp. 553. Plenum Press, New York, USA.
Youn, H. J., Kang, Y. B. and Jang, D. H. (1993). Effects of g-irradiation from cobalt-60 on pathogenicity of Eimeria tenella. Korean Journal of Veterinary Research 33, 649655.

Keywords

Anticoccidial activity of herbal complex in broiler chickens challenged with Eimeria tenella

  • MUHAMMAD A. ZAMAN (a1), ZAFAR IQBAL (a1), RAO Z. ABBAS (a1) (a2) and MUHAMMAD N. KHAN (a1)

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