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Anthelmintic activity of Stevia multiaristata extract against Echinococcus granulosus sensu stricto

Published online by Cambridge University Press:  13 December 2021

C. M. Albani Open the ORCID record for C. M. Albani [Opens in a new window] ,
J. Borgo ,
J. Fabbri ,
P. Pensel ,
L. Fasciani ,
O. Elso ,
D. Papademetrio ,
D. Grasso ,
A. Paladini  and
M. F. Beer
...Show all authors
C. M. Albani*
Affiliation:
Facultad de Ciencias Exactas y Naturales – UNMdP, Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM CONICET-UNMdP); Centro Científico Tecnológico Mar del Plata – CONICET; Centro de Asociación Simple CIC PBA, Mar del Plata, Argentina Laboratorio de Zoonosis Parasitarias, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad Nacional de Mar del Plata (UNMdP), Mar del Plata, Buenos Aires, Argentina
J. Borgo
Affiliation:
CONICET, Instituto de Química y Metabolismo del Fármaco (IQUIMEFA), Universidad de Buenos Aires, Buenos Aires, Argentina Facultad de Farmacia y Bioquímica, Departamento de Farmacología, Cátedra de Farmacognosia, Universidad de Buenos Aires, Buenos Aires, Argentina
J. Fabbri
Affiliation:
Facultad de Ciencias Exactas y Naturales – UNMdP, Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM CONICET-UNMdP); Centro Científico Tecnológico Mar del Plata – CONICET; Centro de Asociación Simple CIC PBA, Mar del Plata, Argentina Laboratorio de Zoonosis Parasitarias, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad Nacional de Mar del Plata (UNMdP), Mar del Plata, Buenos Aires, Argentina
P. Pensel
Affiliation:
Facultad de Ciencias Exactas y Naturales – UNMdP, Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM CONICET-UNMdP); Centro Científico Tecnológico Mar del Plata – CONICET; Centro de Asociación Simple CIC PBA, Mar del Plata, Argentina Laboratorio de Zoonosis Parasitarias, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad Nacional de Mar del Plata (UNMdP), Mar del Plata, Buenos Aires, Argentina
L. Fasciani
Affiliation:
Laboratorio de Zoonosis Parasitarias, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad Nacional de Mar del Plata (UNMdP), Mar del Plata, Buenos Aires, Argentina
O. Elso
Affiliation:
CONICET, Instituto de Química y Metabolismo del Fármaco (IQUIMEFA), Universidad de Buenos Aires, Buenos Aires, Argentina Facultad de Farmacia y Bioquímica, Departamento de Farmacología, Cátedra de Farmacognosia, Universidad de Buenos Aires, Buenos Aires, Argentina
D. Papademetrio
Affiliation:
CONICET, Instituto de Estudios de la Inmunidad Humoral (IDEHU), Universidad de Buenos Aires, Buenos Aires, Argentina Facultad de Farmacia y Bioquímica, Departamento de Microbiología, Inmunología, Biotecnología y Genética, Cátedra de Inmunología, Universidad de Buenos Aires, Buenos Aires, Argentina
D. Grasso
Affiliation:
CONICET, Instituto de Estudios de la Inmunidad Humoral (IDEHU), Universidad de Buenos Aires, Buenos Aires, Argentina Facultad de Farmacia y Bioquímica, Departamento de Ciencias Biológicas, Cátedra de Fisiopatología, Universidad de Buenos Aires, Buenos Aires, Argentina
A. Paladini
Affiliation:
Facultad de Ciencias Veterinarias (UNLP), Cátedra de Parasitología Comparada, Buenos Aires, Argentina
M. F. Beer
Affiliation:
CONICET, Instituto de Química y Metabolismo del Fármaco (IQUIMEFA), Universidad de Buenos Aires, Buenos Aires, Argentina Facultad de Farmacia y Bioquímica, Departamento de Farmacología, Cátedra de Farmacognosia, Universidad de Buenos Aires, Buenos Aires, Argentina
N. E. Farias
Affiliation:
Laboratorio de Invertebrados, Instituto de Investigaciones Marinas y Costeras (IIMYC) (UNMDP-CONICET), Mar del Plata, Argentina
N. Elissondo*
Affiliation:
Laboratorio de Análisis Clínicos, Santisteban, 7000 Tandil, Buenos Aires, Argentina
G. Gambino
Affiliation:
Laboratorio de Análisis Clínicos, Santisteban, 7000 Tandil, Buenos Aires, Argentina
J. Zoppi
Affiliation:
Hospital Privado de Comunidad, Mar del Plata, Buenos Aires, Argentina
V. Sülsen
Affiliation:
CONICET, Instituto de Química y Metabolismo del Fármaco (IQUIMEFA), Universidad de Buenos Aires, Buenos Aires, Argentina Facultad de Farmacia y Bioquímica, Departamento de Farmacología, Cátedra de Farmacognosia, Universidad de Buenos Aires, Buenos Aires, Argentina
M. C. Elissondo
Affiliation:
Facultad de Ciencias Exactas y Naturales – UNMdP, Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM CONICET-UNMdP); Centro Científico Tecnológico Mar del Plata – CONICET; Centro de Asociación Simple CIC PBA, Mar del Plata, Argentina Laboratorio de Zoonosis Parasitarias, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad Nacional de Mar del Plata (UNMdP), Mar del Plata, Buenos Aires, Argentina
*
Authors for correspondence: C. M. Albani, E-mail: albaniclara@gmail.com; M. C. Elissondo, E-mail: c.elissondo@gmail.com
Authors for correspondence: C. M. Albani, E-mail: albaniclara@gmail.com; M. C. Elissondo, E-mail: c.elissondo@gmail.com
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Abstract

Cystic echinococcosis is a zoonotic disease caused by the larval stage of the parasite Echinococcus granulosus sensu lato. The available anti-parasitic treatment is mostly limited to a continuous administration of albendazole. However, due to its numerous side-effects and efficacy of around 50%, there is a need to find new drugs to improve the treatment for this disease. In the current study, the in vitro and in vivo efficacy of a Stevia multiaristata extract against E. granulosus sensu stricto (s.s.) was demonstrated. Stevia multiaristata extract (100 and 50 μg mL−1) caused a quick viability decrease on protoscoleces which was consistent with the observed tegumental alterations. Loss of turgidity was detected in 95 ± 3.4% of cysts incubated with S. multiaristata extract during 2 days (100 μg mL−1) and the collapse of the germinal layer was observed in 60 ± 9.3% of cysts treated with 100 μg mL−1 of the S. multiaristata extract during 4 days. The half maximal effective concentration value was 69.6 μg mL−1 and the selectivity index for E. granulosus s.s. cysts was 1.9. In this clinical efficacy study, the treatment of infected mice with the S. multiaristata extract (50 mg kg−1) caused a significant decrease in the weight of the cysts compared with the control group. These results coincided with the tissue damage observed in the cysts at the ultrastructural level. In conclusion, we observed high protoscolicidal and cysticidal effects, and significant reduction in the weight of the cysts in experimentally infected mice following treatment with the S. multiaristata extract.

Keywords

Anti-parasitic treatmentcystic echinococcosisphytotherapyplant extract
Type
Research Article
Information
Parasitology , Volume 149 , Issue 4 , April 2022 , pp. 519 - 528
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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