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An ultrastructural study of developing stages of exo-erythrocytic Plasmodium berghei in rat hepatocytes

Published online by Cambridge University Press:  06 April 2009

J. F. G. M. Meis ,
J. P. Verhave ,
P. H. K. Jap ,
F. Hess  and
J. H. E. T. Meuwissen
J. F. G. M. Meis
Affiliation:
Departments of Medical Parasitology, University of Nijmegen, Geert Grooteplein Zuid 24/Noord 21, Nijmegen, The Netherlands
J. P. Verhave
Affiliation:
Departments of Medical Parasitology, University of Nijmegen, Geert Grooteplein Zuid 24/Noord 21, Nijmegen, The Netherlands
P. H. K. Jap
Affiliation:
Departments of Cytology and Histology, University of Nijmegen, Geert Grooteplein Zuid 24/Noord 21, Nijmegen, The Netherlands
F. Hess
Affiliation:
Departments of Cytology and Histology, University of Nijmegen, Geert Grooteplein Zuid 24/Noord 21, Nijmegen, The Netherlands
J. H. E. T. Meuwissen
Affiliation:
Departments of Medical Parasitology, University of Nijmegen, Geert Grooteplein Zuid 24/Noord 21, Nijmegen, The Netherlands
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Summary

The ultrastructure of immature exo-erythrocytic forms of Plasmodium berghei in rat hepatocytes was studied at stages between 25 and 51 h of development. A new method was successfully applied to localize the parasites in a small portion of the liver by temporary ligature of blood vessels to the majority of the liver, and from the spleen and the pancreas. Nuclear profiles appeared to be part of a highly lobed nuclear reticulum. Peripheral vesiculation and vacuolization of the cytoplasm was increasingly prominent and ushered in the formation of pseudocytomeres. Spacing between host- and parasite-derived membranes could first be observed after 43 h of development. In general, organellar development was found to follow closely that described for plasmodial oocysts.

Type
Research Article
Information
Parasitology , Volume 82 , Issue 2 , April 1981 , pp. 195 - 204
Copyright
Copyright © Cambridge University Press 1981

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References

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