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Shedding of Cryptosporidium in calves and dams: evidence of re-infection and shedding of different gp60 subtypes

Published online by Cambridge University Press:  22 July 2019

Sarah Thomson Open the ORCID record for Sarah Thomson [Opens in a new window] ,
Elisabeth A. Innes ,
Nicholas N. Jonsson  and
Frank Katzer
Sarah Thomson
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, EH26 0PZ, UK College of Medical, Veterinary and Life Sciences, University of Glasgow, 464 Bearsden Rd, Glasgow G61 1QH, UK
Elisabeth A. Innes
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, EH26 0PZ, UK
Nicholas N. Jonsson
Affiliation:
College of Medical, Veterinary and Life Sciences, University of Glasgow, 464 Bearsden Rd, Glasgow G61 1QH, UK
Frank Katzer*
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, EH26 0PZ, UK
*
Author for correspondence: Frank Katzer, E-mail: frank.katzer@moredun.ac.uk
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Abstract

One of the most common causes of calf diarrhoea is the parasite Cryptosporidium parvum. Two longitudinal studies were carried out on a dairy farm Scotland to determine the prevalence of Cryptosporidium species and subtypes in a group of calves and to determine whether dams were a possible source of calfhood infection. Fecal samples were collected from 25 calves from birth to 12 months in the first year. In the second year, fecal samples were collected from pregnant cows (n = 29) and their calves (n = 30) from birth to 6 months. The samples were tested for Cryptosporidium and speciated. Cryptosporidium parvum-positive samples were subtyped by GP60 fragment analysis. All calves in both studies shed Cryptosporidium during the study period. Cryptosporidium parvum was the predominant species detected in calves ⩽6 weeks of age and at 6 months of age, C. bovis and C. ryanae were detected in calves older than 4 weeks of age but ⩽6 months of age. The prevalence of Cryptosporidium was higher in younger animals than in older animals. GP60 subtyping revealed two subtypes in calves on this farm (IIaA15G2R1 and IIaA19G2R1) that differed in frequency by age. Adult cattle also shed C. parvum, of four gp60 genotypes.

Keywords

C. parvumdam-calf transmissionGP60 subtype
Type
Research Article
Information
Parasitology , Volume 146 , Issue 11 , September 2019 , pp. 1404 - 1413
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Copyright
Copyright © Cambridge University Press 2019 

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