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A kinetoplast DNA probe diagnostic for Leishmania infantum

Published online by Cambridge University Press:  06 April 2009

M. Gramiccia
Affiliation:
Laboratorio di Parassitologia, Istituto Superiore di Sanità, vials Regina Elena 299, 00161 Rome, Italy
D. F. Smith
Affiliation:
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AY, U.K.
M. C. Angelici
Affiliation:
Laboratorio di Parassitologia, Istituto Superiore di Sanità, vials Regina Elena 299, 00161 Rome, Italy
P. D. Ready
Affiliation:
Department of Entomology, The Natural History Museum, London SW7 5BD, U.K.
L. Gradoni
Affiliation:
Laboratorio di Parassitologia, Istituto Superiore di Sanità, vials Regina Elena 299, 00161 Rome, Italy

Extract

A 196 bp fragment of cloned kinetoplast (k) DNA of Leishmania infantum has been sequenced and shown to be diagnostic for this species. The DNA from 104–105 promastigotes was routinely and specifically detected in crude dot blots of whole cells lysed in situ, and there was no cross-hybridization with 106 cells of L. major, L. tropica, L. killicki, L. aethiopica, L. braziliensis and L. mexicana. A low degree of hybridization was found with L. donovani and L. chagasi. This probe will be of particular use in Mediterranean countries, such as Italy, in which dermotropic zymodemes of L. infantum can be difficult to isolate and grow in numbers sufficient for isoenzyme typing.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1992

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References

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