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Crotoxin stimulates an M1 activation profile in murine macrophages during Leishmania amazonensis infection

Published online by Cambridge University Press:  23 June 2017

L. H. S. FARIAS
Affiliation:
Laboratory of Parasitology and Laboratory of Structural Biology, Federal University of Para, Institute of Biological Sciences, Belém, Pará, Brazil National Institute of Science and Technology in Structural Biology and Bioimaging, Rio de Janeiro, Rio de Janeiro, Brazil
A. P. D. RODRIGUES
Affiliation:
National Institute of Science and Technology in Structural Biology and Bioimaging, Rio de Janeiro, Rio de Janeiro, Brazil Laboratory of Electron Microscopy, Department of Health Surveillance, Ministry of Health, Evandro Chagas Institute, Belém, Pará, Brazil
E. C. COÊLHO
Affiliation:
Laboratory of Parasitology and Laboratory of Structural Biology, Federal University of Para, Institute of Biological Sciences, Belém, Pará, Brazil
M. F. SANTOS
Affiliation:
Cell and Developmental Biology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, São Paulo, Brazil
S. C. SAMPAIO
Affiliation:
Laboratory of Pathophysiology, Butantan Institute, São Paulo, Brazil Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
E. O. SILVA
Affiliation:
Laboratory of Parasitology and Laboratory of Structural Biology, Federal University of Para, Institute of Biological Sciences, Belém, Pará, Brazil National Institute of Science and Technology in Structural Biology and Bioimaging, Rio de Janeiro, Rio de Janeiro, Brazil
Corresponding

Summary

American tegumentary leishmaniasis is caused by different species of Leishmania. This protozoan employs several mechanisms to subvert the microbicidal activity of macrophages and, given the limited efficacy of current therapies, the development of alternative treatments is essential. Animal venoms are known to exhibit a variety of pharmacological activities, including antiparasitic effects. Crotoxin (CTX) is the main component of Crotalus durissus terrificus venom, and it has several biological effects. Nevertheless, there is no report of CTX activity during macrophage – Leishmania interactions. Thus, the main objective of this study was to evaluate whether CTX has a role in macrophage M1 polarization during Leishmania infection murine macrophages, Leishmania amazonensis promastigotes and L. amazonensis-infected macrophages were challenged with CTX. MTT [3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl tetrasodium bromide] toxicity assays were performed on murine macrophages, and no damage was observed in these cells. Promastigotes, however, were affected by treatment with CTX (IC50 = 22·86 µg mL−1) as were intracellular amastigotes. Macrophages treated with CTX also demonstrated increased reactive oxygen species production. After they were infected with Leishmania, macrophages exhibited an increase in nitric oxide production that converged into an M1 activation profile, as suggested by their elevated production of the cytokines interleukin-6 and tumour necrosis factor-α and changes in their morphology. CTX was able to reverse the L. amazonensis-mediated inhibition of macrophage immune responses and is capable of polarizing macrophages to the M1 profile, which is associated with a better prognosis for cutaneous leishmaniasis treatment.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2017 

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Crotoxin stimulates an M1 activation profile in murine macrophages during Leishmania amazonensis infection
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