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Identification of Blastocystis subtypes in clinical stool samples from Sao Paulo City, Brazil

  • GESSICA B. MELO (a1) (a2), FABIANA M. PAULA (a1) (a2), FERNANDA M. MALTA (a2) (a3), CELINA W. MARUTA (a4), PAULO R. CRIADO (a4), VERA L. P. CASTILHO (a5), ELENICE M. N. GONÇALVES (a5), MARIA C. ESPIRITO SANTO (a1) and RONALDO C. B. GRYSCHEK (a1) (a2)...

Summary

Blastocystis sp. is a protozoan commonly found in human and animal stool samples. Several pathogenic and zoonotic aspects of this organism are still unknown. The aim of the present study was to investigate Blastocystis subtypes (STs) in samples from patients of the Hospital das Clínicas of the Faculdade de Medicina at the Universidade de São Paulo (HC-FMUSP), Brazil. Blastocystis sp.-positive stool samples diagnosed at the Section of Parasitology of the Central Laboratory (HC-FMUSP) were used for DNA isolation. Polymerase chain reaction (PCR) was performed using specific primers targeting the small-subunit rRNA gene. Direct DNA sequencing of the PCR products was performed and the DNA sequences were then aligned and compared with other sequences obtained from the GenBank database. Phylogenetic analysis was used to identify STs and determine the phylogenetic relationships between the sequences. Four STs were identified: ST1 (22·5%), ST2 (12·5%), ST3 (60%) and ST6 (5%). In conclusion, ST3 was the most prevalent ST among the human isolates followed by ST1. The present study is one of the few providing STs data from the human population in South America. Determining ST prevalence in human samples may contribute to the monitoring of Blastocystis sp. infection transmission in endemic regions.

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This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is unaltered and is properly cited. The written permission of Cambridge University Press must be obtained for commercial re-use or in order to create a derivative work.

Corresponding author

*Corresponding author: Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, USP, São Paulo, Brazil and Instituto de Medicina Tropical, prédio II, 2° andar, Av. Dr Enéas de Carvalho Aguiar, 470, Cerqueira Cesar, 05403-000, São Paulo, SP, Brazil. E-mail: rcbgry@usp.br

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Keywords

Identification of Blastocystis subtypes in clinical stool samples from Sao Paulo City, Brazil

  • GESSICA B. MELO (a1) (a2), FABIANA M. PAULA (a1) (a2), FERNANDA M. MALTA (a2) (a3), CELINA W. MARUTA (a4), PAULO R. CRIADO (a4), VERA L. P. CASTILHO (a5), ELENICE M. N. GONÇALVES (a5), MARIA C. ESPIRITO SANTO (a1) and RONALDO C. B. GRYSCHEK (a1) (a2)...

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