We report on the first population found in Tanzania of the Vulnerable African golden cat Caracal aurata, extending its documented range c. 200 km to the south and south-east. This is one of the least-known and truly forest-dependent felines in Africa, ranging across the Guinea–Congolian forest block. We recorded the new population in Minziro Nature Forest Reserve, north-west Tanzania, during a 3-month survey in 2018. We deployed 70 camera traps on a regular grid and obtained 33 detection events of the golden cat at 26% of sites, with a minimum of 10 individuals across 257 km2. We estimated occupancy and detection probability and modelled these in relation to the distance of sampling sites to the forest edge, which coincides with both the Reserve boundary and proximity to human settlements surrounding the Reserve. Mean estimated occupancy was 0.41 ± SE 0.12 (mean detectability = 0.13 ± SE 0.05), with occupancy increasing significantly with distance from the forest edge. Detectability did not vary significantly with distance from the forest edge, but was higher for camera models that had a shorter trigger time. Our findings add to the scant data available for this species. It appears threatened by human activity, which we recorded both outside and within the Reserve, and the presence of the species indicates Minziro Forest is an important site for its conservation.

Freshwater habitats in China are potentially suitable for invasive alien turtle species and, consequently, raising turtles in aquaculture facilities and the trade in turtles this supplies pose risks to habitats and native wetland communities when exotic turtles escape or are released deliberately. Online trade (e-commerce) is making an increasing contribution to turtle sales in China, seemingly driving demand and thus potentially exacerbating the risk of release. We document the scale and spatial pattern of online sales of non-native turtles over 90 days on China's Taobao.com e-commerce site. The majority of sales were in the ecologically sensitive middle and lower Yangtze river basin (82.35% of > 840,000 slider turtles Trachemys scripta elegans, and 68.26% of > 100,000 snapping turtles, Chelydridae spp.). These species are native to the Americas. Concurrently, over 2008–2018, we found 104 mentions of feral turtle issues listed on Baidu News where, among the 53 prefectures mentioned, issues with invasive turtle populations also focused predominantly in the middle and lower Yangtze river basin. Although circumstantial, this association suggests that the substantial online sale of alien turtles could be having detrimental effects in China's Yangtze river basin. It is important to safeguard these wetland habitats, which are of global importance, by improving policies for detecting and regulating invasive alien turtle issues and by warning consumers about the ecological hazard of their purchases.

The Nilgiri tahr Nilgiritragus hylocrius is an Endangered species of mountain ungulate endemic to the Western Ghats of India, a biodiversity hotspot. Habitat fragmentation, hunting and a restricted range are the major threats to this species. Although several surveys have assessed the species’ status, a population estimate based on a scientifically robust method is needed. We used the double-observer method to estimate the population of the Nilgiri tahr in the Anamalai Tiger Reserve, a protected area in the Western Ghats. We walked 257 km of transects across the Reserve, covering 36 grassland blocks (i.e. clusters of montane grasslands that were relatively separate from each other). We counted a minimum of 422 individuals in 28 groups, and estimated the tahr population in the study area to be 510 individuals (95% CI 300–858) in 35 groups. The male:female ratio was 0.71 and the young:female ratio was 0.56. Comparing our estimate with previous surveys suggests that the Nilgiri tahr population in Anamalai Tiger Reserve is stable. We found the double-observer survey method to be appropriate for population estimation and long-term monitoring of this species, and make recommendations for improved field protocols to facilitate the implementation of the method in the tropical mountains of the Western Ghats. Our findings suggest that the Reserve harbours 20–25% of the global population of the Nilgiri tahr, highlighting the area's importance for the conservation of this species.

There is a long history of exploitation of the South American river turtle Podocnemis expansa. Conservation efforts for this species started in the 1960s but best practices were not established, and population trends and the number of nesting females protected remained unknown. In 2014 we formed a working group to discuss conservation strategies and to compile population data across the species’ range. We analysed the spatial pattern of its abundance in relation to human and natural factors using multiple regression analyses. We found that > 85 conservation programmes are protecting 147,000 nesting females, primarily in Brazil. The top six sites harbour > 100,000 females and should be prioritized for conservation action. Abundance declines with latitude and we found no evidence of human pressure on current turtle abundance patterns. It is presently not possible to estimate the global population trend because the species is not monitored continuously across the Amazon basin. The number of females is increasing at some localities and decreasing at others. However, the current size of the protected population is well below the historical population size estimated from past levels of human consumption, which demonstrates the need for concerted global conservation action. The data and management recommendations compiled here provide the basis for a regional monitoring programme among South American countries.

Throughout the Himalaya, mountain ungulates are threatened by hunting for meat and body parts, habitat loss, and competition with livestock. Accurate population estimates are important for conservation management but most of the available methods to estimate ungulate densities are difficult to implement in mountainous terrain. Here, we tested the efficacy of the recent extension of the point transect method, using camera traps for estimating density of two mountain ungulates: the group-living Himalayan blue sheep or bharal Pseudois nayaur and the solitary Himalayan musk deer Moschus leucogaster. We deployed camera traps in 2017–2018 for the bharal (summer: 21 locations; winter: 25) in the trans-Himalayan region (3,000–5,000 m) and in 2018–2019 for the musk deer (summer: 30 locations; winter: 28) in subalpine habitats (2,500–3,500 m) in the Upper Bhagirathi basin, Uttarakhand, India. Using distance sampling with camera traps, we estimated the bharal population to be 0.51 ± SE 0.1 individuals/km2 (CV = 0.31) in summer and 0.64 ± SE 0.2 individuals/km2 (CV = 0.37) in winter. For musk deer, the estimated density was 0.4 ± SE 0.1 individuals/km2 (CV = 0.34) in summer and 0.1 ± SE 0.05 individuals/km2 (CV = 0.48) in winter. The high variability in these estimates is probably a result of the topography of the landscape and the biology of the species. We discuss the potential application of distance sampling with camera traps to estimate the density of mountain ungulates in remote and rugged terrain, and the limitations of this method.

Darwin's frogs Rhinoderma darwinii and Rhinoderma rufum are the only known species of amphibians in which males brood their offspring in their vocal sacs. We propose these frogs as flagship species for the conservation of the Austral temperate forests of Chile and Argentina. This recommendation forms part of the vision of the Binational Conservation Strategy for Darwin's Frogs, which was launched in 2018. The strategy is a conservation initiative led by the IUCN SSC Amphibian Specialist Group, which in 2017 convened 30 governmental, non-profit and private organizations from Chile, Argentina and elsewhere. Darwin's frogs are iconic examples of the global amphibian conservation crisis: R. rufum is categorized as Critically Endangered (Possibly Extinct) on the IUCN Red List, and R. darwinii as Endangered. Here we articulate the conservation planning process that led to the development of the conservation strategy for these species and present its main findings and recommendations. Using an evidence-based approach, the Binational Conservation Strategy for Darwin's Frogs contains a comprehensive status review of Rhinoderma spp., including critical threat analyses, and proposes 39 prioritized conservation actions. Its goal is that by 2028, key information gaps on Rhinoderma spp. will be filled, the main threats to these species will be reduced, and financial, legal and societal support will have been achieved. The strategy is a multi-disciplinary, transnational endeavour aimed at ensuring the long-term viability of these unique frogs and their particular habitat.

With human influences driving populations of apex predators into decline, more information is required on how factors affect species at national and global scales. However, camera-trap studies are seldom executed at a broad spatial scale. We demonstrate how uniting fine-scale studies and utilizing camera-trap data of non-target species is an effective approach for broadscale assessments through a case study of the brown hyaena Parahyaena brunnea. We collated camera-trap data from 25 protected and unprotected sites across South Africa into the largest detection/non-detection dataset collected on the brown hyaena, and investigated the influence of biological and anthropogenic factors on brown hyaena occupancy. Spatial autocorrelation had a significant effect on the data, and was corrected using a Bayesian Gibbs sampler. We show that brown hyaena occupancy is driven by specific co-occurring apex predator species and human disturbance. The relative abundance of spotted hyaenas Crocuta crocuta and people on foot had a negative effect on brown hyaena occupancy, whereas the relative abundance of leopards Panthera pardus and vehicles had a positive influence. We estimated that brown hyaenas occur across 66% of the surveyed camera-trap station sites. Occupancy varied geographically, with lower estimates in eastern and southern South Africa. Our findings suggest that brown hyaena conservation is dependent upon a multi-species approach focussed on implementing conservation policies that better facilitate coexistence between people and hyaenas. We also validate the conservation value of pooling fine-scale datasets and utilizing bycatch data to examine species trends at broad spatial scales.

Pangolins are the most trafficked animals globally. Although the Indian pangolin Manis crassicaudata occurs across the Indian subcontinent (excluding parts of the Himalayas and the north-east, where the Chinese pangolin Manis pentadactyla is present), it is categorized as Endangered on the IUCN Red List as a result of heavy poaching for its meat and scales. Several areas have not yet been surveyed for the occurrence of the Indian pangolin, one of which is the 16,948 km2 tropical deciduous forests of the northern Eastern Ghats landscape in Andhra Pradesh. During December 2017–April 2018, we surveyed 750 km2 of this landscape for the presence of the Indian pangolin, using camera traps. As an alternative method to document pangolin presence, and to identify factors driving hunting of the species, we also conducted, during the same period, a total of 60 semi-structured interviews with people in 30 villages. Interviewees reported the presence of pangolins in a majority of the grid cells that we surveyed with camera traps, particularly in moist deciduous forests distant from villages. However, the camera traps did not detect pangolins in 840 trap-days. Hunting of pangolins for their meat, which is consumed locally, and for their scales, which are traded, is most likely the reason for the rarity of the species in this landscape. A better understanding of the proximate and ultimate drivers of pangolin hunting is required, to inform proactive conservation management for this increasingly threatened species.

Frogs have been harvested from the wild for the last 40 years in Turkey. We analysed the population dynamics of Anatolian water frogs (Pelophylax spp.) in the Seyhan and Ceyhan Deltas during 2013–2015. We marked a total of 13,811 individuals during 3 years, estimated population sizes, simulated the dynamics of a harvested population over 50 years, and collated frog harvest and export statistics from the region and for Turkey as a whole. Our capture estimates indicated a population reduction of c. 20% per year, and our population modelling showed that, if overharvesting continues at current rates, the harvested populations will decline rapidly. Simulations with a model of harvested population dynamics resulted in a risk of extinction of > 90% within 50 years, with extinction likely in c. 2032. Our interviews with harvesters revealed their economic dependence on the frog harvest. However, our results also showed that reducing harvest rates would not only ensure the viability of these frog populations but would also provide a source of income that is sustainable in the long term. Our study provides insights into the position of Turkey in the ‘extinction domino’ line, in which harvest pressure shifts among countries as frog populations are depleted and harvest bans are effected. We recommend that harvesting of wild frogs should be banned during the mating season, hunting and exporting of frogs < 30 g should be banned, and harvesters should be trained on species knowledge and awareness of regulations.

Around the world, Indigenous peoples have stories about wildlife that reflect knowledge and feelings about animals and their relationship to humans. Different people's experiences speak to the variety of interactions people have with animals in the spaces where humans and non-human animals live and interact. These stories are often told by women, reflecting the ways in which gender mediates human–environment relations. Yet gendered differences in knowledge and experience are rarely addressed in wildlife conservation research and action. Even community-based conservation efforts often ignore or marginalize the knowledge and experiences of women. We present women's stories and experiences of wildlife from Maasai communities in Tanzania and Soliga communities in India. We show that women have the desire and knowledge to participate in conservation decision-making but are currently marginalized from community conservation practice. We argue that including women in research and action is key for successful community-based wildlife conservation.

Carnivores play an important role in ecosystem functioning as apex predators. However, most carnivore species are threatened or have been extirpated in human-dominated landscapes. The Mediterranean region of central Chile is a biodiversity hotspot, but expansion of agricultural areas such as vineyards is degrading wildlife habitat. We estimated the species richness and composition of carnivore communities in remnant fragments of sclerophyllous forest-shrubland in the vineyard landscapes of central Chile to evaluate the effects of human disturbance at different spatial scales. We tested two hypotheses: (1) vineyard landscapes with higher levels of human disturbance support a lower diversity of native carnivores in fragments of remnant native vegetation compared to landscapes with larger areas of natural habitat, and (2) habitat specialists and generalists respond differentially to human influence at the habitat vs landscape spatial scale. We used camera traps at 12 sites across the study area and evaluated the impact of human disturbance indicators on the richness and detection frequency of carnivore species. We found that human population density negatively affected carnivore richness and was associated with a lower detection frequency of the Vulnerable guiña Leopardus guigna. The presence of domestic dogs also had a negative effect on the detection frequency of the guiña and the two native species of foxes, the culpeo Lycalopex culpaeus and South American grey fox Lycalopex griseus. We conclude that protecting remnants of native forest in vineyard landscapes is crucial for carnivore conservation in central Chile.

Reliably estimating population parameters for highly secretive or rare animals is challenging. We report on the status of the two largest remaining populations of the Critically Endangered Bermuda skink Plestiodon longirostris, using a robust design capture–mark–recapture analysis. Skinks were tagged with passive integrated transponders on two islands and captured on 15 sampling occasions per year over 3 years. The models provided precise estimates of abundance, capture and survival probabilities and temporary emigration. We estimated skink abundance to be 547 ± SE 63.5 on Southampton Island and 277 ± SE 28.4 on Castle Island. The populations do not appear to be stable and fluctuated at both sites over the 3-year period. Although the populations on these two islands appear viable, the Bermuda skink faces population fluctuations and remains threatened by increasing anthropogenic activities, invasive species and habitat loss. We recommend these two populations for continued monitoring and conservation efforts.

The effectiveness of many widely used conservation interventions is poorly understood because of a lack of high-quality impact evaluations. Randomized control trials (RCTs), in which experimental units are randomly allocated to treatment or control groups, offer an intuitive way to calculate the impact of an intervention by establishing a reliable counterfactual scenario. As many conservation interventions depend on changing people's behaviour, conservation impact evaluation can learn a great deal from RCTs in fields such as development economics, where RCTs have become widely used but are controversial. We build on relevant literature from other fields to discuss how RCTs, despite their potential, are just one of a number of ways to evaluate impact, are not feasible in all circumstances, and how factors such as spillover between units and behavioural effects must be considered in their design. We offer guidance and a set of criteria for deciding when RCTs may be an appropriate approach for evaluating conservation interventions, and factors to consider to ensure an RCT is of high quality. We illustrate this with examples from one of the few concluded RCTs of a large-scale conservation intervention: an incentive-based conservation programme in the Bolivian Andes. We argue that conservation should aim to avoid a rerun of the polarized debate surrounding the use of RCTs in other fields. Randomized control trials will not be feasible or appropriate in many circumstances, but if used carefully they can be useful and could become a more widely used tool for the evaluation of conservation impact.

Sacred forests or groves are patches of forest vegetation that are traditionally protected by local communities because of their religious or cultural significance. The ecological aspects of sacred forests have been the focus of most of the scholarly discourse; little scholarship has examined how local people perceive their sacred areas. This scholarly lacuna is especially pronounced with respect to women, as the majority of sacred forests have traditionally been the domain of the men. Until recently, the sacred forests tradition in most regions endured with minimal participation of women, but with changing socio-economic and cultural conditions, sacred forests are declining. By examining women's perspectives regarding their relationship with their sacred forests, this research informs the scholarship on gender and sacred forests, and explores the role women can play in forest conservation. In 2015–2017, we conducted village meetings and in-depth interviews in four villages located in and around the Bhimashankar Wildlife Sanctuary in the Western Ghats region of Maharashtra state, India. We found that apart from rules and taboos governing the protection of these sacred forests, taboos also revolve around the access and interaction of women with the sacred forests, with women having less control and decision-making power than men. Nevertheless, women expressed interest in continuation of the tradition of sacred forests, and the younger generation wants some of the gendered rules to change. We recommend including women in management and decision-making processes to strengthen the institution of sacred forests.

Caves and other subterranean habitats are crucial for the survival of many bat species, but often deteriorate as a result of visits by tourists. The aim of the study was to understand the conservation challenges associated with the cave dwelling bats at Gupteswar cave tourism and pilgrimage site in eastern India and to develop conservation recommendations. I counted bat populations and monitored tourist visits once per month for 12 months during September 2016–August 2017. Roosting and breeding activities of eight species of bats, including two nationally threatened species, were recorded from five caves. The number of bats counted during the 12 survey days was 785–940 individuals. Tourism activity occurred throughout the year but was higher during local festive seasons; the maximum number of tourist entries recorded in a single day was 2,769. Installation of gated entrances, scheduling of visits to control overcrowding, restriction of access to caves with maternity colonies during breeding seasons, and minimal use of electric bulbs for illumination would minimize disturbance to the bats. Installation of educational display boards would help to create awareness of the conservation importance of bats amongst the cave visitors.

Over recent decades, Chinese giant salamanders Andrias spp. have declined dramatically across much of their range. Overexploitation and habitat degradation have been widely cited as the cause of these declines. To investigate the relative contribution of each of these factors in driving the declines, we carried out standardized ecological and questionnaire surveys at 98 sites across the range of giant salamanders in China. We did not find any statistically significant differences between water parameters (temperature, dissolved oxygen, ammonia, nitrite, nitrate, salinity, alkalinity, hardness and flow rate) recorded at sites where giant salamanders were detected by survey teams and/or had been recently seen by local respondents, and sites where they were not detected and/or from which they had recently been extirpated. Additionally, we found direct and indirect evidence that the extraction of giant salamanders from the wild is ongoing, including within protected areas. Our results support the hypothesis that the decline of giant salamanders across China has been primarily driven by overexploitation. Data on water parameters may be informative for the establishment of conservation breeding programmes, an initiative recommended for the conservation of these species.