With the advent of automated recording units, bioacoustic monitoring has become a popular tool for the collection of long-term data across extensive landscapes. Such methods involve two main components: hardware for audio data acquisition and software for analysis. In the acoustic monitoring of threatened species, a species-specific framework is often essential. Jerdon's courser Rhinoptilus bitorquatus is a Critically Endangered nocturnal bird endemic to a small region of the Eastern Ghats of India, last reported in 2008. Here we describe a reproducible and scalable acoustic detection framework for the species, comparing several commonly available hardware and detection methods and using existing software. We tested this protocol by collecting 24,349 h of data during 5 months. We analysed the data with two commercially available sound analysis programmes, following an analysis pipeline created for this species. Although we did not detect vocalizations of Jerdon's courser, this study provides a framework using a combination of hardware and software for future research that other conservation practitioners can implement. Vocal mimicry can aid or confound in detection and we highlight the potential role of mimicry in the detection of such threatened species. This species-specific acoustic detection framework can be scaled and tailored to monitor other species.

We analysed 23 years of data on strandings of the Antillean manatee Trichechus manatus manatus in Belize, documented by the Belize Marine Mammal Stranding Network, to examine the threats to this population. A total of 451 stranding incidents were reported, of which 376 (83.4%) cases were verified. A total of 286 (63.4%) of the incidents occurred within Belize District, where the number of strandings has almost tripled since 2009. Watercraft collisions accounted for the highest number of strandings, with 131 confirmed cases, and is the leading cause of anthropogenic mortality for this population. Collision with watercraft is an emerging and major threat to manatees in Belize, and is correlated with increases in human activity, in particular associated with tourism. This finding of high levels of manatee deaths in Belize is consistent with trends previously reported for manatees in Florida and Puerto Rico. This work can provide guidance to detect and address similar patterns of mortality in other Antillean manatee populations across the species' range. There is a need for greater awareness of the threats facing the species and its habitat, for stakeholder partnerships to address these threats, implementation of legislation for the protection of manatees, and consistent enforcement of regulations to protect this population. Boating regulations, such as no-wake zones within areas of high manatee presence, as well as regulation of tourism boating activities, need to be implemented to reduce the threats to the species.

The Vulnerable marsh deer Blastocerus dichotomus, the largest native cervid in South America, is declining throughout its range as a result of the conversion of wetlands and overhunting. Estimated densities in open wetlands of several types are 0.1–6.8 individuals per km2. We undertook the first unmanned aerial vehicle (UAV) survey of the marsh deer to estimate the density of this species in a 113.6 km2 area under forestry management in the lower delta of the Paraná River, Argentina. During 6–8 August 2019, at a time of year when canopy cover is minimal, we surveyed marsh deer using Phantom 4 Pro UAVs along 94 transects totalling 127.8 km and 8.6 km2 (8.1% of the study area). The 5,506 photographs obtained were manually checked by us and by a group of 39 trained volunteers, following a standardized protocol. We detected a total of 58 marsh deer, giving an estimated density of 6.90 individuals per km2 (95% CI 5.26–8.54), which extrapolates to 559–908 individuals in our 113.6 km2 study area. As it has generally been assumed that marsh deer prefer open habitats, this relatively high estimate of density within a forestry plantation matrix is unexpected. We discuss the advantages of using UAVs to survey marsh deer and other related ungulates.

The IUCN now recognizes the savannah Loxodonta africana and forest Loxodonta cyclotis elephants to be separate species. Despite ecological, behavioural and morphological differences, and different habitat ranges, genetic studies confirm that the two species and hybrids coexist in forest–savannah ecotones. However, the hybrid phenotypes have not yet been described. In this survey we examined whether the phenotypes of the two species and of hybrids can be distinguished. In the first step, we used a machine learning algorithm (K-nearest neighbours) to compare 296 reference images of African elephants from five forest areas and six savannah areas where hybrids have not been recorded, confirming that six morphological criteria can be used to distinguish the species with more than 90% confidence. In the second step, we analysed 1,408 videos of elephants from 14 camera traps in Sebitoli, in Kibale National Park, Uganda, part of the main hybridization area. We used a multiple correspondence analysis and a species assignment key, highlighting the presence of three categories of phenotypes. Compared to the savannah and forest phenotypes (36.8 and 12.1%, respectively), the intermediate phenotypes, which could include hybrids, were more frequent (51.1%). Further studies combining morphology and genetics of the same individuals will be necessary to refine this species assignment key to characterize phenotypes confidently. This non-invasive, fast and inexpensive phenotypical-based method could be a valuable tool for conservation programmes.

The recovery of species’ populations is one of the most challenging tasks in conservation, which is why species recovery is most likely to be successful if it involves as many stakeholders as possible. Podocarpus angustifolius, a tree endemic to Cuba, is potentially threatened by overexploitation, deforestation and expansion of exotic species. In December of 2017, in two protected areas containing the only known subpopulations of this species, we recorded the geographical position, height, and diameter at breast height of each individual, and calculated the extent of occurrence (EOO) and area of occupancy (AOO). We recorded a total of 575 individuals, 174 of which were adult (defined as ≥ 6 m height on the basis of the occurrence of reproductive structures), and most of which occurred in Lomas de Banao Ecological Reserve. The EOO and AOO were 125.8 km2 and 6.0 km2, respectively. All P. angustifolius that remain in the wild are within two protected areas, either in rainforest relicts or in coffee plantations. The size structure indicates a growing population. Based on our findings we recommend that P. angustifolius should be recategorized from Vulnerable (on the IUCN Red List) and Critically Endangered (on the national Red List) to Endangered, based on criterion D (small population size). We recommend the continued reinforcement of the population at Topes de Collantes Natural Protected Landscape and the continued engagement of local stakeholders for the long-term conservation of this species.

Large carnivores are frequently released for conservation purposes, but early efforts struggled with inadequate monitoring and reporting, resulting in poor understanding of success. Although managers have improved release practice and monitoring, the use of orphaned, captive-raised large carnivores for release remains controversial because of the potential influence of captivity and the possible lack of natural behaviours in such individuals. Yet, rehabilitating orphaned individuals for release could help mitigate pressures on vulnerable wild populations. We present a case study on the rearing, rehabilitation and release of wild-born cheetahs Acinonyx jubatus that were rescued as orphans in Namibia. Our aim was to develop a methodological framework for cheetah release planning and post-release management based on the outcome of release trials conducted during 2004–2018. Between 2001 and 2012, we rescued 86 orphaned cheetahs from the wild. Of these, 36 (42%) were selected as release candidates. We found high success rates (75–96%) of selected individuals in achieving independence post-release. Additionally, annual survival estimates for rehabilitated individuals that reached independence were comparable to those of wild counterparts described by other studies, and some rehabilitated individuals reproduced with wild conspecifics. Our findings demonstrate the ability of wild-born, captive-raised cheetahs to transition back into the wild with strategic pre- and post-release management directed towards optimizing survival. This includes selecting appropriate release candidates, forming artificial coalitions, balancing habituation levels during captivity, choosing appropriate release sites, and providing strategic support during post-release monitoring. We encourage scientists and managers to implement and refine our protocol for rehabilitation throughout the cheetah's current and historic range.

Conservation action plans need to be devised and implemented if we are to reduce the extinction risk faced by globally threatened plants. However, most plant species categorized as threatened globally on the IUCN Red List lack conservation action plans. In West Africa, Guinea is one of the most diverse countries in terms of botanical species. In total, 273 plant species in Guinea have been assessed as being threatened globally, reflecting increasing pressure from the extractive industry and a growing population requiring food and fuel. In parallel with the implementation of an Important Plant Area programme in Guinea, we developed conservation action plans for 20 threatened plant species through a pilot study. We outline the methods we used and demonstrate the importance of adopting a collaborative approach and having up-to-date field information. The need for such plans is urgent, with recent estimates suggesting that one-third of African plants are threatened with extinction. Based on our experience with the first 20 conservation action plans for Guinea species, we suggest that the preparation of multi-species conservation action plans would be an efficient use of the limited resources available for species conservation.

Worldwide, conservation initiatives have attempted to curb illegal harvest, use and trade of marine turtles at least since the 1950s. Despite some declines in local trade and consumption, these illegal activities are still often reported as a key threat to marine turtle populations. Reassessing and refining global conservation priorities for marine turtles allows us to formulate evidence-based strategies and effective interventions to address this threat. We surveyed a total of 103 marine turtle conservation researchers and practitioners globally to understand how conservation efforts can be better allocated to curb illegal harvest, use and trade. We explored the characteristics of these illegal activities, conservation priorities, challenges and lessons learnt. According to participants’ perceptions, progress has been achieved, but illegal harvest, use and trade remain pressing threats globally. Current challenges to addressing illegal activities relate to fisheries management, enforcement and legislation. Recommended priority actions include law and penalty enforcement, enhancing environmental literacy, awareness and stakeholder participation, and improving local conservation leadership and onshore/maritime management based on research. Based on participants' perceptions, we identify priorities for marine turtle conservation interventions that aim to curb illegal harvest, use and trade. Given the challenges of obtaining reliable information on sensitive topics such as illegal harvest, use and trade, further work should seek to validate our findings through empirical research. Further work could also seek to comprehend better how expert elicitation in conservation is influenced by individual experience, perspectives and goals.

Pallas's fish eagle Haliaeetus leucoryphus was recategorized from Vulnerable to Endangered on the IUCN Red List in 2017 because of evidence that there is only a single population, which is declining as a result of continuous, widespread loss and degradation of freshwater wetlands. To determine the species’ status in Bangladesh, we conducted a large-scale community-based interview survey in north-east Bangladesh in 2017–2020. We also examined nest site habitat characteristics through field surveys and remotely sensed data. We conducted a total of 955 interviews in an area of 4,150 km2, through which we were able to determine the presence of 53 breeding pairs at a mean density of 1.2 nests per 100 km2. There was a higher nest density (3.7–4.8 nests per 100 km2) in some locations, which we identify as priority conservation areas. The majority of nests (62.2%) were close together and on tall trees with an open canopy structure. Nests were located within or close to (< 100 m) human settlements, and within 500 m of wetlands and rivers. Felling of nest trees, removal of nests by local people and loss of permanent wetlands (14.6% during 2010–2020) appeared to be the main threats. High nesting density in our study area suggests that the freshwater wetlands in north-east Bangladesh possibly hold the largest population of Pallas's fish eagle globally.

The northern bald ibis Geronticus eremita disappeared from Europe in the Middle Ages. Since 2003 a migratory population has been reintroduced in Central Europe. We conducted demographic analyses of the survival and reproduction of 384 northern bald ibises over a period of 12 years (2008–2019). These data also formed the basis for a population viability analysis simulating the possible future development of the northern bald ibis population under different scenarios. We analysed life stage-specific survival rates, rearing protocols and colonies, and the influence of stochastic catastrophic events and reinforcement translocations on population growth. Life stage-specific survival probabilities were 0.64–0.78. Forty-five per cent of the mature females reproduced, with a mean fecundity of 2.15 fledglings per nest. The complementary population viability analysis indicated that the Waldrappteam population is close to self-sustainability, with an estimated population growth rate of 0.95 and a 24% extinction probability within 50 years. Of the 326 future scenarios tested, 94% reached the criteria of extinction probabilities < 5% and population growth rates > 1. Stochastic catastrophic events had only a limited effect. Despite comparatively high survival and fecundity rates the population viability analysis indicated that to achieve self-sustainability the Waldrappteam population needs further translocations to support population growth and the implementation of effective measures against major mortality threats: illegal hunting in Italy and electrocution on unsecured power poles. The findings of this study are to be implemented as part of a second European LIFE project.

The Endangered giant pangolin Smutsia gigantea is rare and elusive across its Central African range. Because of its solitary and nocturnal nature, the species is difficult to study and so its ecology is little known. Pangolins are considered the most trafficked mammals in the world. Therefore, confirming presence accurately and monitoring trends in distribution and abundance are essential to inform and prioritize conservation efforts. Camera traps are popular tools for surveying rare and cryptic species. However, non-targeted camera-trap surveys yield low camera-trapping rates for pangolins. Here we use camera-trap data from surveys conducted within three protected areas in Uganda to test whether targeted placement of cameras improves giant pangolin detection probability in occupancy models. The results indicate that giant pangolin detection probability is highest when camera traps are targeted on burrows. The median number of days from camera deployment to first giant pangolin detection event was 12, with the majority of events captured within 32 days from deployment. The median interval between giant pangolin events at a camera-trap site was 33 days. We demonstrate that camera-trap surveys can be designed to improve the detection of giant pangolins and we outline a set of recommendations to maximize the effectiveness of efforts to survey and monitor the species.

As threats facing wildlife and protected areas across Africa increase, demand for innovative and transformational leadership to tackle the challenges remains high. Traditional academic training programmes are playing a critical role in meeting capacity development needs, yet opportunities for strengthening leadership capabilities are limited. This was the rationale behind Mentoring for ENvironmental Training in Outreach and Resource conservation (MENTOR), initiated in 2007 by the U.S. Fish & Wildlife Service through a collaborative effort with various partners to support conservation leadership and capacity development across sub-Saharan Africa. Five independent programmes were implemented over a decade, each designed to combine rigorous academic and field-based training with mentoring and experiential learning for teams of 8–9 fellows selected through a competitive process. It was envisioned that this approach to leadership and capacity development would strengthen the resolve, capabilities and competences of the fellows and position them as conservation leaders. Using data from interviews and online surveys, we assessed three key aspects of the programmes: strategic relevance and design; progress, effectiveness and impact; and sustainability. Overall, we found that all five programmes successfully delivered the objective of strengthening leadership for conservation in Africa, with the cadre of professionals acquiring new skills and expertise to advance their careers, and developing life-long relationships and networks. We discuss the potential of this approach for developing African conservation leaders.

We used citizen science and inexpensive methodology to assess the distribution of the jungle cat Felis chaus, a relatively common species in Sri Lanka but the least studied of the four wild cat species occurring in the country. We obtained three types of records of the jungle cat: geo-referenced photographs of the species from the public; sightings obtained from print and social media, and provided via an online sighting form; and sightings by field biologists. We combined the 112 unique records obtained in this way with the 21 records from the 2012 National Red List distribution map of the species, and used MaxEnt to predict habitat suitability for the species. The new sightings were primarily in drier regions, expanding the known extent of occurrence for this species in Sri Lanka. Of the new sightings, 7.1% were road kills. Distance to nearest riverine forest, annual precipitation and distance to the nearest reservoir were the most important variables explaining habitat suitability. These findings validate our hypotheses that the species is more widespread than demonstrated previously and also ranges in human-dominated landscapes outside protected areas. Our study provides a model for how ecological and behavioural information for common species can be obtained inexpensively and incorporated into species distribution models. Studies of species such as the jungle cat, which are neither threatened nor charismatic, will help ensure that we keep common species common.

The Atlantic Forest of South America supports a rich terrestrial biodiversity but has been reduced to only a small extent of its original forest cover. It hosts a large number of endemic mammalian species but our knowledge of arboreal mammal ecology and conservation has been limited because of the challenges of observing arboreal species from ground level. Camera trapping has proven to be an effective tool in terrestrial mammal monitoring but the technique has rarely been used for arboreal species. For the first time in the Atlantic Forest, we obtained data on the arboreal mammal community using arboreal camera trapping, focusing on Caparaó National Park, Brazil. We placed 24 infrared camera traps in the forest canopy in seven areas within the Park, operating them continuously during January 2017–June 2019. During this period the camera traps accumulated 4,736 camera-days of footage and generated a total of 2,256 photographs and 30-s videos of vertebrates. The arboreal camera traps were able to detect arboreal mammals of a range of body sizes. The mammal assemblage comprised 15 identifiable species, including the Critically Endangered northern muriqui Brachyteles hypoxanthus and buffy-headed marmoset Callithrix flaviceps as well as other rare, nocturnal and inconspicuous species. We confirmed for the first time the occurrence of the thin-spined porcupine Chaetomys subspinosus in the Park. Species richness varied across survey areas and forest types. Our findings demonstrate the potential of arboreal camera trapping to inform conservation strategies.

Worldwide, nature-based tourism is becoming more popular and important economically. However, there is still debate regarding its impact on wildlife in protected areas. We conducted a quasi-experimental study to investigate the effects of tourism on the mammal community of Cavernas do Peruaçu National Park, a priority area for conservation in Brazil. We used camera traps to survey tourist and non-tourist trails during 2011–2017, encompassing periods before and after tourism started in the Park. We used four metrics for assessment: species richness, probability of using trails, activity levels and daily activity patterns. After tourism began in the Park there was no significant change in species richness and the probability of using tourist trails either increased or remained stable for five of the six species assessed. The rock cavy Kerodon rupestris was the only species to be displaced from tourist areas and to show reduced overall activity on tourist trails after tourism began. The ocelot Leopardus pardalis showed reduced diurnal activity on tourist trails, an indication of temporal adjustment. Overall, our results show that the initial years of visitation at the Park had limited negative impacts on the target mammal species, supporting the possibility of accommodating tourism activity and effective conservation of wildlife in the region. However, it is essential to continue monitoring in the Park because of the expected growth in tourism and potential time lags in responses of species.