Skip to main content Accessibility help
×
Home

Presence of the Endangered Amur tiger Panthera tigris altaica in Jilin Province, China, detected using non-invasive genetic techniques

  • Anthony Caragiulo (a1), Yang Kang (a2), Salisa Rabinowitz (a1), Isabela Dias-Freedman (a3), Simone Loss (a1), Xu-Wei Zhou (a2), Wei-Dong Bao (a2) and George Amato (a1)...

Abstract

China is home to three subspecies of tiger Panthera tigris but there are no estimates of the size of any of the populations. We detected a population of the Endangered Amur tiger Panthera tigris altaica in Hunchun Nature Reserve in Jilin Province using both mitochondrial DNA and nuclear microsatellite loci. Four male and one female tigers were detected, indicating the potential for a small breeding group. However, genetic diversity was low overall, with six loci showing a heterozygote deficiency and a mean of 2.55 alleles per locus. This study is the first estimate of the wild Amur tiger population in China to use non-invasive techniques, and the presence of a female tiger indicates this is a potentially viable population. We provide baseline genetic diversity estimates to support monitoring of the population. The small number of tiger scats located indicates the importance of continuing the current conservation efforts for this tiger subspecies in Hunchun Nature Reserve. Such efforts include reducing poaching of tigers and their prey, and implementation of management plans to encourage the persistence and recovery of tigers in this area.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Presence of the Endangered Amur tiger Panthera tigris altaica in Jilin Province, China, detected using non-invasive genetic techniques
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Presence of the Endangered Amur tiger Panthera tigris altaica in Jilin Province, China, detected using non-invasive genetic techniques
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Presence of the Endangered Amur tiger Panthera tigris altaica in Jilin Province, China, detected using non-invasive genetic techniques
      Available formats
      ×

Copyright

Corresponding author

(Corresponding author) E-mail acaragiulo@amnh.org
(Corresponding author) E-mail wdbao@bjfu.edu.cn

References

Hide All
Benson, D.A., Karsch-Mizrachi, I., Lipman, D.J., Ostell, J. & Wheeler, D.L. (2005) GenBank. Nucleic Acids Research, 33, D34D38.
Bhagavatula, J. & Singh, L. (2006) Genotyping faecal samples of Bengal tiger Panthera tigris tigris for population estimation: a pilot study. BMC Genetics, 7, 112.
Caragiulo, A., Dias-Freedman, I., Clark, J.A., Rabinowitz, S. & Amato, G. (2014) Mitochondrial DNA sequence variation and phylogeography of Neotropic pumas (Puma concolor). Mitochondrial DNA, 25, 304312.
Chen, J., Na, S., Shun, Q., Zhang, L., Tang, J., Lang, J. et al. (2011) Amur tiger and prey in Jilin Hunchun National Nature Reserve, China. Chinese Journal of Zoology, 46, 4652.
Dinerstein, E., Loucks, C., Wikramanayake, E., Ginsberg, J., Sanderson, E., Seidensticker, J. et al. (2007) The fate of wild tigers. BioScience, 57, 508514.
Frankham, R., Ballou, J.D. & Briscoe, D. (2010) Introduction to Conservation Genetics. Cambridge University Press, Cambridge, UK.
Henry, P., Miquelle, D., Sugimoto, T., McCullough, D.R., Caccone, A. & Russello, M.A. (2009) In situ population structure and ex situ representation of the endangered Amur tiger. Molecular Ecology, 18, 31733184.
Heptner, V. & Sludskii, A. (1972) Mammals of the Soviet Union, Volume II, Part 2 Carnivora (Hyaenas and Cats), pp. 83159. Smithsonian Institution Libraries and National Science Foundation, Washington, DC, USA.
Höglund, J., Johansson, T., Beintema, A. & Schekkerman, H. (2009) Phylogeography of the black-tailed godwit Limosa limosa: substructuring revealed by mtDNA control region sequences. Journal of Ornithology, 150, 4553.
Kalinowski, S., Taper, M. & Marshall, T. (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Molecular Ecology, 16, 10991106.
Luo, S.J., Johnson, W.E., Martenson, J., Antunes, A., Martelli, P., Uphyrkina, O. et al. (2008) Subspecies genetic assignments of worldwide captive tigers increase conservation value of captive populations. Current Biology, 18, 592596.
Luo, S.J., Kim, J.H., Johnson, W.E., Walt, J., Martenson, J. & Karanth, U.K. (2004) Phylogeography and genetic ancestry of tigers (Panthera tigris). PLoS Biology, 2, 22752293.
Ma, Y., Zhang, E., Miquelle, D., Wang, T. & Kang, A. (2005) Changes in Amur tiger distribution in northeast China in the past 100 years. In Recovery of the Wild Amur Tiger Population in China: Progress and Prospect (eds Zhang, E., Miquelle, D., Wang, T. & Kang, A.), pp. 164170. China Forestry Publishing House, Harbin, China.
Menotti-Raymond, M., David, V., Lyons, L., Schäffer, A., Tomlin, J., Hutton, M. & O'Brien, S. (1999) A genetic linkage map of microsatellites in the domestic cat (Felis catus). Genomics, 57, 923.
Miquelle, D., Darman, Y. & Seryodkin, I. (2011) Panthera tigris altaica. In The IUCN Red List of Threatened Species. v. 2014.2. Http://www.iucnredlist.org [accessed 18 September 2014].
Miquelle, D., Goodrich, J., Kerley, L., Pikunov, D., Dunishenko, Y.M., Aramiliev, V. et al. (2010) Science-based conservation of Amur tigers in the Russian Far East and north-east China. In Tigers of the World: The Science, Politics, and Conservation of Panthera tigris (eds Tilson, R. & Nyhus, P.), pp. 403423. Elsevier/Academic Press, Oxford, UK.
Miquelle, D., Pikunov, D., Dunishenko, Y., Aramilev, V., Nikolaev, I., Abramov, V. et al. (2005) Amur tiger census. CAT News, 46, 1114.
Mondol, S., Karanth, K.U., Kumar, N.S., Gopalaswamy, A.M., Andheria, A. & Ramakrishnan, U. (2009) Evaluation of non-invasive genetic sampling methods for estimating tiger population size. Biological Conservation, 142, 23502360.
Morell, V. (2007) Can the wild tiger survive? Science, 317, 13121314.
Pikunov, D.G., Miquelle, D.G., Abramov, V.K., Nikolaev, I.G., Seryodkin, I.V., Murzin, A.A. & Korkiskho, V.G. (2003) Results of population surveys of leopards (Panthera pardus orientalis) and tigers (Panthera tigris altaica) in south-west Primorskii Krai, Russian Far East. Final report to The Wildlife Conservation Society and The World Wildlife Fund.
Ratnasingham, S. & Hebert, P.D.N. (2007) Bold: the barcode of life data system (http://www.barcodinglife.org). Molecular Ecology Notes, 7, 355364.
Rousset, F. (2008) GENEPOP'007: a complete re-implementation of the GENEPOP software for Windows and Linux. Molecular Ecology Resources, 8, 103106.
Russello, M.A., Gladyshev, E., Miquelle, D. & Caccone, A. (2004) Potential genetic consequences of a recent bottleneck in the Amur tiger of the Russian Far East. Conservation Genetics, 5, 707713.
STATE FORESTRY ADMINISTRATION (2009) Resources Survey on National Key Terrestrial Wild Animals, pp. 265271. China Forestry Publishing House, Beijing, China. [In Chinese]
Sugimoto, T., Nagata, J., Aramilev, V.V. & McCullough, D.R. (2012) Population size estimation of Amur tigers in Russian Far East using noninvasive genetic samples. Journal of Mammalogy, 93, 93101.
Sun, H. & Dong, H. (2005) The wild population monitor of Amur tiger in Heilongjiang Province. Forestry Science and Technology, 6, 3335.
Sun, B., Miquelle, D., Xiaochen, Y., Zhang, E., Hiyai, S., Goshen, G. et al. (1999) Survey of Amur Tigers and Far Eastern Leopards in Eastern Heilongjiang Province, China, and Recommendations for their Conservation. A final report to The Wildlife Conservation Society, Bronx, New York, USA.
Taberlet, P., Griffin, S., Goossens, B., Questiau, S., Manceau, V., Escaravage, N. et al. (1996) Reliable genotyping of samples with very low DNA quantities using PCR. Nucleic Acids Research, 24, 31893194.
Valière, N. (2002) GIMLET: a computer program for analysing genetic individual identification data. Molecular Ecology Notes, 2, 377379.
Wei, K., Zhang, Z., Zhang, W., Xu, X., Liang, X., He, G. et al. (2008) PCR-CTPP: a rapid and reliable genotyping technique based on ZFX/ZFY alleles for sex identification of tiger (Panthera tigris) and four other endangered felids. Conservation Genetics, 9, 225228.
Yang, S., Jiang, J., Wu, Z., Li, T., Yang, X., Han, X. et al. (1998) A Survey of Tigers and Leopards in Eastern Jilin Province, China, Winter 1998. A final report to UNDP and The Wildlife Conservation Society, Vladivostok, Russia.

Keywords

Type Description Title
PDF
Supplementary materials

Caragiulo supplementary material
Caragiulo supplementary material

 PDF (118 KB)
118 KB

Presence of the Endangered Amur tiger Panthera tigris altaica in Jilin Province, China, detected using non-invasive genetic techniques

  • Anthony Caragiulo (a1), Yang Kang (a2), Salisa Rabinowitz (a1), Isabela Dias-Freedman (a3), Simone Loss (a1), Xu-Wei Zhou (a2), Wei-Dong Bao (a2) and George Amato (a1)...

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed