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The role of glial cells in influencing neurite extension by dorsal root ganglion cells

Published online by Cambridge University Press:  22 December 2009

Kai-Yu Ng ,
Yung H. Wong  and
Helen Wise
Kai-Yu Ng
Affiliation:
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
Yung H. Wong
Affiliation:
Department of Biochemistry, Hong Kong University of Science and Technology, Hong Kong SAR, China
Helen Wise*
Affiliation:
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
*
Correspondence should be addressed to: Helen Wise, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China phone: +852 2603 6849 fax: +852 2603 5139 email: helenwise@cuhk.edu.hk
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Abstract

When pretreated with pertussis toxin (PTX), the neurites of adult rat dorsal root ganglion (DRG) cells in mixed cell cultures retract over a period of 2 h following the initial stimulus of removal from the cell culture incubator for brief periods of observation. The purpose of this investigation was to determine whether this PTX-dependent response was specific to any one of the three subpopulations of DRG neurons. However, no neurite retraction response was observed in neuron-enriched populations of cells, or in cultures enriched in isolectin B4 (IB4)-positive neurons or in IB4-negative neurons. But, the addition of non-neuronal cells, and/or medium conditioned by non-neuronal cells, was sufficient to restore the PTX-dependent neurite retraction response, but only in large diameter IB4-negative neurons. In conclusion, we have identified a regulatory response, mediated by Gi/o-proteins, which prevents retraction of neurites in large diameter IB4-negative cells of adult rat DRG. The non-neuronal cells of adult rat DRG constitutively release factor/s that can stimulate neurite retraction of a subset of isolated DRG neurons, but this property of non-neuronal cells is only observed when the Gi/o-proteins of large diameter IB4-negative cells are inhibited.

Keywords

Neurite retractionGi/o proteins
Type
Research Article
Information
Neuron Glia Biology , Volume 6 , Special Issue 1: Satellite Glial Cells , February 2010 , pp. 19 - 29
Copyright
Copyright © Cambridge University Press 2009

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