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Variation in rDNA ITS sequences in Glomus mosseae and Gigaspora margarita spores from a permanent pasture

Published online by Cambridge University Press:  01 June 2000

Z. I. ANTONIOLLI ,
D. P. SCHACHTMAN ,
K. OPHEL-KELLER  and
S. E. SMITH
Z. I. ANTONIOLLI
Affiliation:
Department of Soil and Water, The University of Adelaide, PMB 1, Glen Osmond, South Australia 5064, Australia
D. P. SCHACHTMAN
Affiliation:
CSIRO Plant Industry, GPO Box 350, Glen Osmond, South Australia 5064, Australia
K. OPHEL-KELLER
Affiliation:
Primary Industries and Resources South Australia, PMB 1, Glen Osmond, South Australia 5064, Australia. E-mail: Sally.Smith@adelaide.edu.au
S. E. SMITH
Affiliation:
Department of Soil and Water, The University of Adelaide, PMB 1, Glen Osmond, South Australia 5064, Australia
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Abstract

To study the genetic variability in Glomus mosseae and Gigaspora margarita sequence similarities of ITS regions of rDNA were analysed from spores collected from a permanent pasture and from pot cultures. PCR amplification with the primers ITS1 and ITS4 was performed and products were cloned and sequenced. The sequences from single spores of G. mosseae and Gi. margarita confirmed that there is variation in the ITS region in a single spore. Phenetic analysis of sequences from both species supported the morphological identification which placed the species into two separate groups. Through the analysis of these sequences an estimate of genetic diversity was derived which clearly showed that the three field spores of G. mosseae were at least 2–5 times more genetically diverse than one single spore (field) and a pool of spores (pot culture) of Gigaspora margarita. This demonstrates that a high degree of variation exists in this natural population of G. mosseae.

Type
Research Article
Information
Mycological Research , Volume 104 , Issue 6 , June 2000 , pp. 708 - 715
Copyright
© The British Mycological Society 2000

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