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The use of fatty acid signatures to study mycelial interactions between the arbuscular mycorrhizal fungus Glomus intraradices and the saprotrophic fungus Fusarium culmorum in root-free soil

Published online by Cambridge University Press:  01 December 1998

JOHN LARSEN ,
PÅL AXEL OLSSON  and
IVER JAKOBSEN
JOHN LARSEN
Affiliation:
Plant Biology and Biogeochemistry Department, Risø National Laboratory, DK-4000 Roskilde, Denmark Present address: Department of Plant Pathology and Pest Management, Danish Institute of Agricultural Sciences, Ministry of Food, Agriculture and Fisheries, Flakkebjerg, DK-4200 Slagelse, Denmark.
PÅL AXEL OLSSON
Affiliation:
Department of Microbial Ecology, Lund University, Ecology Building, S-223 62 Lund, Sweden
IVER JAKOBSEN
Affiliation:
Plant Biology and Biogeochemistry Department, Risø National Laboratory, DK-4000 Roskilde, Denmark
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Abstract

The saprotrophic fungus Fusarium culmorum, Penicillium hordei, Rhizoctonia solani and Trichoderma harzianum and the arbuscular mycorrhizal fungus Glomus intraradices were examined for content of phospholipid fatty acids (PLFA) and neutral lipid fatty acids (NLFA). The AM fungus differed from the saprotrophic fungi especially by its content of the fatty acid 16[ratio ]1ω5 which was absent in the saprotrophs. The fatty acid 18[ratio ]2ω6,9 was the dominant fatty acid of the saprotrophic fungi while it was negligible in mycelium of G. intraradices. Specificity in content of fatty acids made it possible to quantify G. intraradices and F. culmorum simultaneously in soil. Furthermore, a compartmented growth system made it possible to study mycelial interactions in the absence of roots. We measured hyphal spread of both fungi, hyphal 33P transport of G. intraradices and sporulation of F. culmorum. The two fungi did not interact according to the parameters used in this study. We conclude that fatty acid signatures may be a valuable tool when studying interactions between AM fungi and other fungi in root-free soil.

Type
Research Article
Information
Mycological Research , Volume 102 , Issue 12 , December 1998 , pp. 1491 - 1496
Copyright
© The British Mycological Society 1998

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