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Molecular identification of white morphotype strains of endophytic fungi from Pinus tabulaeformis

Published online by Cambridge University Press:  01 August 2003

Liang Dong GUO ,
Guo R. HUANG ,
Yu WANG ,
Wen H. HE ,
Wei H. ZHENG  and
Kevin D. HYDE
Liang Dong GUO
Affiliation:
Systematic Mycology & Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China. E-mail: guold@sun.im.ac.cn
Guo R. HUANG
Affiliation:
Systematic Mycology & Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China. E-mail: guold@sun.im.ac.cn
Yu WANG
Affiliation:
Systematic Mycology & Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China. E-mail: guold@sun.im.ac.cn
Wen H. HE
Affiliation:
Systematic Mycology & Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China. E-mail: guold@sun.im.ac.cn
Wei H. ZHENG
Affiliation:
Systematic Mycology & Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China. E-mail: guold@sun.im.ac.cn
Kevin D. HYDE
Affiliation:
Department of Ecology & Biodiversity, Centre for Research in Fungal Diversity, The University of Hong Kong, Pokfulam Road, Hong Kong, S.A.R., People's Republic of China.
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Abstract

Sterile mycelia isolated from Pinus tabulaeformis were grouped into white morphotype strains based on cultural characteristics. Eighteen of the isolates were randomly selected and identified to various taxonomic levels based on nuclear ribosomal DNA (nrDNA) sequence analysis. The 5.8S gene and flanking internal transcribed spacer (ITS1 and ITS2) regions of nrDNA were amplified and sequenced. Phylogenetic analysis of the 5.8S gene sequences indicated that the white morphotype strains were Ascomycota. Further identification was achieved by means of sequence similarity comparison and phylogenetic analysis of the ITS regions. Results showed that strains WMS9 and WMS10 were Lophodermium species (Rhytismataceae), while strains WMS11, WMS13 and WMS18 were species of Rhytismataceae. Strains WMS2, WMS3, WMS4, WMS5 and WMS6 were identified to Rosellinia, strain WMS1 to Entoleuca, and strain WMS14 to Nemania (Xylariaceae). Strains WMS7, WMS8, WMS12, WMS15, WMS16 and WMS17 were xylariaceous species. The potential of using DNA sequence analysis in the identification of endophytic fungi is discussed.

Type
Research Article
Information
Mycological Research , Volume 107 , Issue 6 , June 2003 , pp. 680 - 688
Copyright
© The British Mycological Society 2003

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