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Genetic comparison of the aggressive weed mould strains of Trichoderma harzianum from mushroom compost in North America and the British Isles

Published online by Cambridge University Press:  01 April 1998

S. MUTHUMEENAKSHI ,
A. E. BROWN  and
P. R. MILLS
S. MUTHUMEENAKSHI
Affiliation:
Department of Applied Plant Science, The Queen's University of Belfast Present address: Department of Plant Pathology and Microbiology, HRI, Wellesbourne, Warwick, CV35 9EF.
A. E. BROWN
Affiliation:
Department of Applied Plant Science, The Queen's University of Belfast Applied Plant Science Division, Department of Agriculture for Northern Ireland, Newforge Lane, Belfast BT9 5PX, U.K.
P. R. MILLS
Affiliation:
Department of Applied Plant Science, The Queen's University of Belfast Applied Plant Science Division, Department of Agriculture for Northern Ireland, Newforge Lane, Belfast BT9 5PX, U.K. Present address: Department of Plant Pathology and Microbiology, HRI, Wellesbourne, Warwick, CV35 9EF.
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Abstract

Genetic characterization of 15 aggressive weed mould strains that were identified morphologically as Trichoderma harzianum (sensu Rifai) from mushroom compost in commercial units in North America (designated T. harzianum group 4) was undertaken using various molecular techniques. RFLP analysis of rDNA revealed homogeneity among these strains; a low level of polymorphism was detected in mitochondrial DNA. RAPD analysis also indicated a very high degree of homogeneity among the T. harzianum group 4 strains. Nucleotide sequence determination of the rDNA internal transcribed spacer (ITS) 1 revealed five base pair differences between T. harzianum groups 4 and 2. Comparison of molecular data on T. harzianum group 4 with that of T. harzianum group 2, which caused green mould epidemics in the mushroom industries of the British Isles, indicated that T. harzianum group 4 is not the same as group 2.

Type
Research Article
Information
Mycological Research , Volume 102 , Issue 4 , April 1998 , pp. 385 - 390
Copyright
The British Mycological Society 1998

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