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Evolution of Aspergillus niger and A. nidulans in glucose-limited chemostat cultures, as indicated by oscillations in the frequency of cycloheximide resistant and morphological mutants

Published online by Cambridge University Press:  01 March 2000

Richard J. SWIFT ,
Sally H. CRAIG ,
Marilyn G. Wiebe ,
Geoffrey D. ROBSON  and
Anthony P. J. TRINCI
Richard J. SWIFT
Affiliation:
School of Biological Sciences, 1.800 Stopford Building, University of Manchester, Manchester M13 9PT, UK
Sally H. CRAIG
Affiliation:
School of Biological Sciences, 1.800 Stopford Building, University of Manchester, Manchester M13 9PT, UK
Marilyn G. Wiebe
Affiliation:
School of Biological Sciences, 1.800 Stopford Building, University of Manchester, Manchester M13 9PT, UK
Geoffrey D. ROBSON
Affiliation:
School of Biological Sciences, 1.800 Stopford Building, University of Manchester, Manchester M13 9PT, UK
Anthony P. J. TRINCI
Affiliation:
School of Biological Sciences, 1.800 Stopford Building, University of Manchester, Manchester M13 9PT, UK
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Abstract

Aspergillus niger and A. nidulans were grown separately in glucose-limited chemostat cultures on modified Vogel's medium. Periodic selection (the appearance of new mutant populations) in A. niger was determined by monitoring oscillations in the concentration of cycloheximide-resistant mycelial fragments in samples from the fermenter vessel. Using these data, the interval between the periodic selection of each new mutant population was 32±6 generations (mean±S.E.M.). Periodic selection in cycloheximide resistance in three morphologically distinct sub-populations of the A. niger culture averaged 36±2, 31±11 and 28±5 generations and these values were not significantly different from that of the whole population (32±6 generations). Also, it was possible to estimate the rate of evolution taking place in these cultures by monitoring oscillations in the frequency of morphological mutants. The intervals between the periodic selection of morphological mutant populations in A. niger and A. nidulans were 28±3 and 22±2 generations respectively.

Type
Research Article
Information
Mycological Research , Volume 104 , Issue 3 , March 2000 , pp. 333 - 337
Copyright
© The British Mycological Society 2000

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