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Complementation cloning of salt tolerance determinants from the marine hyphomycete Dendryphiella salina in Aspergillus nidulans

Published online by Cambridge University Press:  01 October 1999

D. J. CLEMENT ,
M. S. STANLEY ,
J. O'NEIL ,
N. A. WOODCOCK ,
D. A. FINCHAM ,
N. J. W. CLIPSON  and
P. HOOLEY
D. J. CLEMENT
Affiliation:
School of Applied Sciences, University of Wolverhampton, Wulfruna Street, Wolverhampton, West Midlands, WV1 1LY, U.K. Current Address: ARC Epidemiology Research Unit, University of Manchester, Manchester, M13 9PG, UK.
M. S. STANLEY
Affiliation:
School of Applied Sciences, University of Wolverhampton, Wulfruna Street, Wolverhampton, West Midlands, WV1 1LY, U.K.
J. O'NEIL
Affiliation:
School of Applied Sciences, University of Wolverhampton, Wulfruna Street, Wolverhampton, West Midlands, WV1 1LY, U.K.
N. A. WOODCOCK
Affiliation:
School of Applied Sciences, University of Wolverhampton, Wulfruna Street, Wolverhampton, West Midlands, WV1 1LY, U.K.
D. A. FINCHAM
Affiliation:
School of Applied Sciences, University of Wolverhampton, Wulfruna Street, Wolverhampton, West Midlands, WV1 1LY, U.K.
N. J. W. CLIPSON
Affiliation:
School of Applied Sciences, University of Wolverhampton, Wulfruna Street, Wolverhampton, West Midlands, WV1 1LY, U.K. Current Address: Department of Industrial Microbiology, University College, Dublin, Belfield, Dublin 4, Eire.
P. HOOLEY
Affiliation:
School of Applied Sciences, University of Wolverhampton, Wulfruna Street, Wolverhampton, West Midlands, WV1 1LY, U.K.
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Abstract

A preliminary physiological characterization of osmoregulation in an Aspergillus nidulans salt sensitive mutant carrying cotransformed DNA sequences from the marine hyphomycete Dendryphiella salina is described. A genomic DNA library made in lambda EMBL3 was cotransformed with the plasmid pDJB3 into an A. nidulans host carrying a selectable marker for transformation (pyrG89) and a salt sensitivity mutation (sltA1). In some cases D. salina clones appeared to directly complement the salt sensitivity mutation by restoring salt and arginine insensitivity. Other clones gave marked increases in salt tolerance and yet the hosts remained arginine sensitive. Improvements in salt tolerance correlated with an enhanced ability to induce polyol biosynthesis during salt challenge.

Type
Research Article
Information
Mycological Research , Volume 103 , Issue 10 , October 1999 , pp. 1252 - 1258
Copyright
The British Mycological Society 1999

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